Application of HAP1 in screening neuroinflammation treatment drugs

A technology for neuroinflammation and drug treatment, applied in the field of biomedicine, can solve the problems of reducing the quality of life of patients, unclear mechanism of occurrence and development, and complex etiology of neuropathic pain.

Pending Publication Date: 2022-06-28
NANTONG UNIVERSITY
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Problems solved by technology

Because neuropathic pain is intense and long-lasting, it often resists all available analgesic measures. It is not only a physical devastation for the patient, but also a psychological torture, which greatly reduces the quality of life of the patient.
At the same time, the etiology of neuropathic pain is complex, and the specific mechanism of its occurrence and development is still not very clear.

Method used

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  • Application of HAP1 in screening neuroinflammation treatment drugs
  • Application of HAP1 in screening neuroinflammation treatment drugs
  • Application of HAP1 in screening neuroinflammation treatment drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Neuropathic pain can induce neuroinflammation

[0027] HAP1 wild-type mice were used to construct an acute pain model, and slices were obtained, followed by immunofluorescence staining.

[0028] GFAP and Iba-1 are specific markers for astrocytes and microglia, respectively. figure 1 The staining results showed that compared with the control side spinal cord, the surgical side spinal cord showed significant glial cell activation phenomenon. This result suggests that neuropathic pain can induce neuroinflammation.

[0029] 2. Low expression of HAP1 can inhibit the activation of glial cells

[0030] The acute pain model was constructed from HAP1 heterozygous mice, and the slices were taken and then subjected to immunofluorescence staining.

[0031] GFAP and Iba-1 are specific markers for astrocytes and microglia, respectively. figure 2The staining results showed that glial cell activation also occurred in the spinal cord of the operated side compared with the contro...

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Abstract

The invention discloses application of HAP1 in screening neuroinflammation treatment drugs, and belongs to the technical field of biological medicines. According to the invention, an HAP1 wild type mouse is selected as a control group, an HAP1 heterozygote mouse is selected as an experimental group, neuropathologic acute pain is constructed, and postoperative inflammatory responses of the two groups of mice are respectively detected. Results show that by reducing the expression of the HAP1, the inflammatory response can be obviously reduced, the activation level of glial cells is reduced, and the release of inflammatory factors is inhibited; and finally, the effect of controlling neuroinflammation is achieved. The discovery can provide a new molecular target for pain research.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to the application of HAP1 in screening neuroinflammation treatment drugs. Background technique [0002] Neuropathic pain is pain caused by damage or disease of the somatosensory nervous system. Neuropathic pain, caused by damage or dysfunction of the nervous system, is one of the most vexing human problems. Epidemiological statistics have found that about 6.9%-10% of chronic pain patients have neuropathic pain symptoms. Because neuropathic pain is intense and persistent, it is often resistant to all available analgesic measures, and is not only physical but psychological torture for patients, greatly reducing the quality of life of patients. At the same time, the etiology of neuropathic pain is complex, and the specific mechanism of its occurrence and development is not very clear. Therefore, in-depth study of the molecular mechanism of neuropathic pain is very im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00C12Q1/6883
CPCA61K49/0008C12Q1/6883C12Q2600/136C12Q2600/158
Inventor 潘静莹杨日云包璟崟吴泳江
Owner NANTONG UNIVERSITY
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