Lipase/polyacrylamide aquagel microsphere catalytic material, preparation method therefor and application of lipase/polyacrylamide aquagel microsphere catalytic material

A technology of polyacrylamide and hydrogel microspheres, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problems of increasing the mass transfer resistance between enzymes and substrates, and achieve low cost, short time consumption, Does not reduce the effect of enzyme activity

Active Publication Date: 2021-06-18
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional hydrogel usually achieves the immobilization effect by forming an encapsulation of the enzyme, which greatly increases the mass transfer resistance between the enzyme and the substrate.

Method used

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  • Lipase/polyacrylamide aquagel microsphere catalytic material, preparation method therefor and application of lipase/polyacrylamide aquagel microsphere catalytic material
  • Lipase/polyacrylamide aquagel microsphere catalytic material, preparation method therefor and application of lipase/polyacrylamide aquagel microsphere catalytic material
  • Lipase/polyacrylamide aquagel microsphere catalytic material, preparation method therefor and application of lipase/polyacrylamide aquagel microsphere catalytic material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Add acrylamide (12g, 168.82mmol), N,N-methylenebisacrylamide (0.12g, 0.78mmol), 0.18g polyvinylpyrrolidone into 40mL of purified water, stir mechanically at 65°C until dissolved , as the aqueous phase;

[0051] (2) Add 8 g of compound emulsifier (Span 80: Tween 80 = 1: 0.3) into 120 mL of cyclohexane, stir mechanically at 65°C until fully mixed, and use it as the organic phase;

[0052] (3) The organic phase and the water phase are mixed, and the reactor is stirred at 500 rpm for 30 min at 65° C. and feeds nitrogen gas to remove oxygen in the solution to prepare a water-in-oil emulsion, add ammonium persulfate (0.12 g, 0.08%), and Inverse emulsion polymerization was carried out at 70°C for 2 hours, and the mechanical stirring rate was kept constant at 350rpm during the polymerization;

[0053] (4) After the reaction, thoroughly wash the prepared polyacrylamide hydrogel microspheres with ethanol and water successively to remove unreacted monomers;

[0054] (5) Weig...

Embodiment 2

[0056] (1) Add acrylamide (12g, 168.82mmol), N,N-methylenebisacrylamide (0.12g, 0.78mmol), 0.18g polyvinylpyrrolidone into 40mL of purified water, stir mechanically at 65°C until dissolved , as the aqueous phase;

[0057] (2) Add 9 g of compound emulsifier (Span 80: Tween 80 = 1: 0.3) into 120 mL of cyclohexane, stir mechanically at 65°C until fully mixed, and use it as the organic phase;

[0058] (3) The organic phase and the water phase are mixed, and the reactor is stirred at 500 rpm for 30 min at 65° C. and feeds nitrogen gas to remove oxygen in the solution to prepare a water-in-oil emulsion, add ammonium persulfate (0.12 g, 0.08%), and Inverse emulsion polymerization was carried out at 70°C for 2 hours, and the mechanical stirring rate was kept constant at 350rpm during the polymerization;

[0059] (4) After the reaction, thoroughly wash the prepared polyacrylamide hydrogel microspheres with ethanol and water successively to remove unreacted monomers;

[0060] (5) Weig...

Embodiment 3

[0062] (1) Add acrylamide (12g, 168.82mmol), N,N-methylenebisacrylamide (0.12g, 0.78mmol), 0.18g polyvinylpyrrolidone into 40mL of purified water, stir mechanically at 65°C until dissolved , as the aqueous phase;

[0063] (2) Add 10 g of compound emulsifier (Span 80: Tween 80 = 1: 0.3) into 120 mL of cyclohexane, mechanically stir at 65°C until fully mixed, and use it as the organic phase;

[0064] (3) The organic phase and the water phase are mixed, and the reactor is stirred at 500 rpm for 30 min at 65° C. and feeds nitrogen gas to remove oxygen in the solution to prepare a water-in-oil emulsion, add ammonium persulfate (0.12 g, 0.08%), and Inverse emulsion polymerization was carried out at 70°C for 2 hours, and the mechanical stirring rate was kept constant at 350rpm during the polymerization;

[0065] (4) After the reaction, thoroughly wash the prepared polyacrylamide hydrogel microspheres with ethanol and water successively to remove unreacted monomers;

[0066] (5) Wei...

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Abstract

The invention provides a lipase / polyacrylamide aquagel microsphere catalytic material, a preparation method therefor and application of the lipase / polyacrylamide aquagel microsphere catalytic material. The method comprises the steps: mixing an aqueous phase containing an acrylamide monomer and N,N-methylene-bisacrylamide with an organic phase containing a compounding emulsifier, and then, carrying out an out-phase emulsion polymerization reaction, so as to obtain polyacrylamide aquagel microspheres; and physically adsorbing lipase to the polyacrylamide aquagel microspheres, thereby obtaining the lipase / polyacrylamide aquagel microsphere catalytic material. The compounding emulsifier is prepared through mixing a Span emulsifier and a Tween emulsifier and has an HLB value of 3-7. The catalytic material prepared by the method is environmentally friendly and non-toxic, is easily separated from a reaction system, can be recycled and thus will have an extensive application value in various fields such as biocatalysis and food industry.

Description

technical field [0001] The invention relates to a lipase / polyacrylamide hydrogel microsphere catalytic material and its preparation method and application, belonging to the field of enzyme immobilization. Background technique [0002] Lipase is an enzyme that has a variety of catalytic capabilities and can catalyze some water-insoluble substrates, so it is widely used in many reactions, such as hydrolysis of ester bonds, synthesis, alcoholysis, ammonolysis, peroxidation, epoxy Because of the high efficiency, strong specificity and mild reaction conditions of the enzyme itself, it has broad application prospects in the fields of food, chemical medicine, and cosmetics production. However, the brittleness and rapid aging of enzymes in non-physiological environments limit their industrial applications, and the "lid" structure formed by the three amino acids serine, aspartic acid, and histidine in the lipase structure is of great importance to the lipase. The combination of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/087C12N9/20C12P7/64C08F220/56C08F222/38C08J3/075
CPCC12N11/087C12N9/20C12Y301/01003C08F220/56C08J3/075C12P7/6436C08F222/385
Inventor 田晶秦正强费旭李尧徐龙权王一
Owner DALIAN POLYTECHNIC UNIVERSITY
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