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Method for preparing coenzyme Q10 by microbial transformation under condition of supercritical CO2

A supercritical, coenzyme technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as reducing yield, and achieve the effects of promoting synthesis, changing oxygen supply methods, and reducing product inhibition.

Inactive Publication Date: 2009-12-02
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first two have not been widely used due to the limitation of raw materials and cost conditions. The microbial conversion method has attracted much attention because of its mild reaction conditions, fast speed and non-toxic and harmless substrates. However, the disadvantage is that it is easily inhibited by the product and reduces the yield.

Method used

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  • Method for preparing coenzyme Q10 by microbial transformation under condition of supercritical CO2
  • Method for preparing coenzyme Q10 by microbial transformation under condition of supercritical CO2

Examples

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Effect test

Embodiment 1

[0020] Embodiment 1, supercritical CO 2 Preparation of Coenzyme Q by Microbial Transformation under Conditional Conditions 10

[0021] 1. Culture of bacteria

[0022] Inoculate Schizosaccharomyces pombe (S.promb, purchased from the General Microorganism Center of China Microbiological Culture Collection Management Committee, product catalog number 2.1794) on the slant activation medium, culture in a constant temperature incubator at 28°C for 24h, and then inoculate into In 50ml of liquid activation medium, culture on a shaker at 28°C for 18h, with a shaker speed of 200r / min, then inoculate 10% of the inoculum into five 250ml Erlenmeyer flasks containing 100ml of fermentation medium respectively, at 200r / min Cultivate in a shaker at 28°C for 18 hours at a constant speed until the end of the logarithmic growth phase.

[0023] Incline activation medium: 2% glucose, 2% peptone, 1% yeast extract, pH 6.0, 2% agar, and the rest is water; the stated percentages are mass percentages...

Embodiment 2

[0034] Embodiment 2, supercritical CO 2 Preparation of Coenzyme Q by Microbial Transformation under Conditional Conditions 10

[0035] 1. Culture of bacteria

[0036] Inoculate Schizosaccharomyces pombe (S.promb, purchased from the General Microorganism Center of China Microbiological Culture Collection Management Committee, product catalog number 2.1794) on the slant activated medium, culture it in a constant temperature incubator at 35°C for 20h, and then inoculate it into In 50ml of liquid activation medium, cultivate on a shaker at 35°C for 24 hours, the shaker speed is 250r / min, and then inoculate 5% of the inoculum into five 250ml Erlenmeyer flasks containing 100ml of fermentation medium respectively, at 250r / min Cultivate in a shaker at 35°C for 24 hours at a constant speed until the end of the logarithmic growth phase.

[0037] Incline activation medium: 2% glucose, 2% peptone, 1% yeast extract, pH 6.5, 2% agar, and the rest is water; the stated percentages are mass...

Embodiment 3

[0046] Embodiment 3, supercritical CO 2 Preparation of Coenzyme Q by Microbial Transformation under Conditional Conditions 10

[0047] 1. Culture of bacteria

[0048] The culture step of bacteria is the same as that described in Step 1 in Example 1.

[0049] Inoculate Schizosaccharomyces pombe (S.promb, purchased from the General Microorganism Center of China Microbiological Culture Collection Management Committee, product catalog number 2.1794) on the slant activation medium, culture in a constant temperature incubator at 30°C for 16h, and then inoculate into In 50ml of liquid activation medium, cultivate on a shaking table at 25°C for 16h, the rotating speed of the shaking table is 150r / min, and then inoculate 5 250ml Erlenmeyer flasks containing 100ml of fermentation medium with 1% inoculation amount, and inoculate at 150r / min Cultivate in a shaker at 25°C for 16 hours at 25 °C until the end of the logarithmic growth phase.

[0050] Incline activation medium: 1% glucose...

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Abstract

The invention discloses a method for preparing coenzyme Q10. The method of the invention comprises the following steps: switching-in schizosaccharomyces pombe to culture medium containing solanesol, carrying out fermentation in the supercritical CO2 environment with temperature ranging from 25 DEG C to 35 DEG C and pressure ranging from 5MPa to 20 MPa, thus obtaining the coenzyme Q10.The method of the invention makes full use of dissolution characteristics of the supercritical CO2 to increase solubility of the solanesol, thus effectively reducing product inhibiting effect in the process of conversion, providing a high anaerobic environment for yeast, changing respiratory chain oxygen supply mode of microorganism, and promoting synthesis of the coenzyme Q10; in addition, in the method of the invention, the solanesol is added as substrate of the coenzyme Q10, thus improving speed and output of the synthesis of the coenzyme Q10.Experiments indicate that the output of the coenzyme Q10 prepared by the method of the invention is increased by 60-162% compared with the output thereof prepared by ordinary fermentation methods.

Description

technical field [0001] The invention relates to a method for preparing coenzyme Q 10 method, especially involving a supercritical CO 2 Preparation of Coenzyme Q by Microbial Transformation under Conditional Conditions 10 Methods. Background technique [0002] Supercritical CO 2 As a reaction and extraction medium, the conditions are mild, there is a large diffusion coefficient, the solubility and dielectric constant are sensitive to temperature and pressure, and it is non-toxic, non-flammable, economical, and does not produce solvent residues, so it can be used as an enzyme-catalyzed reaction. the reaction medium. [0003] coenzyme Q 10 Due to its special physiological and biochemical functions, it is widely used in clinical and health care fields. In recent years, with the deepening of its research, its application fields and demand are expanding. Currently producing coenzyme Q 10 The methods mainly include tissue extraction, chemical synthesis and microbial fermenta...

Claims

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Application Information

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IPC IPC(8): C12P7/66C12N1/16C12R1/645
CPCY02P20/54
Inventor 刘萍孙君社肖霄
Owner CHINA AGRI UNIV
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