Method for preparing coenzyme Q10 by microbial transformation under condition of supercritical CO2

A supercritical, coenzyme technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as reducing yield, and achieve the effects of promoting synthesis, changing oxygen supply methods, and reducing product inhibition.
CN101591685AInactive Publication Date: 2009-12-02CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2009-12-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing coenzyme Q10. The method of the invention comprises the following steps: switching-in schizosaccharomyces pombe to culture medium containing solanesol, carrying out fermentation in the supercritical CO2 environment with temperature ranging from 25 DEG C to 35 DEG C and pressure ranging from 5MPa to 20 MPa, thus obtaining the coenzyme Q10.The method of the invention makes full use of dissolution characteristics of the supercritical CO2 to increase solubility of the solanesol, thus effectively reducing product inhibiting effect in the process of conversion, providing a high anaerobic environment for yeast, changing respiratory chain oxygen supply mode of microorganism, and promoting synthesis of the coenzyme Q10; in addition, in the method of the invention, the solanesol is added as substrate of the coenzyme Q10, thus improving speed and output of the synthesis of the coenzyme Q10.Experiments indicate that the output of the coenzyme Q10 prepared by the method of the invention is increased by 60-162% compared with the output thereof prepared by ordinary fermentation methods.
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Description

technical field

[0001] The invention relates to a method for preparing coenzyme Q 10 method, especially involving a supercritical CO 2 Preparation of Coenzyme Q by Microbial Transformation under Conditional Conditions 10 Methods. Background technique

[0002] Supercritical CO 2 As a reaction and extraction medium, the conditions are mild, there is a large diffusion coefficient, the solubility and dielectric constant are sensitive to temperature and pressure, and it is non-toxic, non-flammable, economical, and does not produce solvent residues, so it can be used as an enzyme-catalyzed reaction. the reaction medium.

[0003] coenzyme Q 10 Due to its special physiological and biochemical functions, it is widely used in clinical and health care fields. In recent years, with the deepening of its research, its application fields and demand are expanding. Currently producing coenzyme Q 10 The methods mainly include tissue extraction, chemical synthesis and microbial fermenta...

Claims

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