Methods and compositions for cellular and metabolic engineering
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
a metabolic engineering and cellular technology, applied in the field of methods and compositions for cellular and metabolic engineering, can solve the problems of unsuitable intracellular location of proteins, unsuitable folding, improper modification, etc., and achieve the effect of improving the ability to catalyz
Inactive Publication Date: 2006-11-16
MAXYGEN
View PDF45 Cites 17 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Benefits of technology
[0025] (4) screening at least one further recombinant gene from the further library of recombinant genes that confers enhanced ability to catalyze the second reaction of interest in the cell relative to a previous recombinant gene;
Problems solved by technology
Bailey (Science 252:1668-1674 (1991)) describes the application of metabolic engineering to the recruitment of heterologous genes for the improvement of a strain, with the caveat that such introduction can result in new compounds that may subsequently undergo further reactions, or that expression of a heterologous protein can result in proteolysis, improper folding, improper modification, or unsuitable intracellular location of the protein, or lack of access to required substrates.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
examples
I. Alteration of Enzyme Activity and Specificity.
[0208] In this example, recursive sequence recombination techniques of the instant invention were used to expand the range of substrates efficiently hydrolyzed by E. coli β-galactosidase. The goal was to evolve wild type E. coli β-galactosidase into a fucosidase. The enzyme showed very weak activity with both ρ-nitrophenyl-β-D-fucopyranoside and o-nitrophenyl-β-D-fucopyranoside (estimated respectively as 80- and 160-fold less efficient than for ρ-nitrophenyl-β-D-galactopyranoside).
[0209] To increase the activity of E. coli β-galactosidase against these fucopyranoside derivatives, a lacZ gene (a 3.8 kb Hind III-BamHI fragment from plasmid pCH110, Pharmacia) encoding E. coli β-galactosidase was subcloned into plasmid p18SFI-BLA-SFI (Stemmer, Nature, 370:389-391 (1994)). The resulting plasmid, p18-lacZ, was used for recursive sequence recombination and mutant screening.
[0210] Purified plasmid p18-lacZ (4-5 μg) was used directly for D...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
temperature
aaaaa
aaaaa
temperature
aaaaa
aaaaa
pH
aaaaa
aaaaa
Login to view more
Abstract
The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to “evolve” individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.
Description
[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 08 / 198,431, filed Feb. 17, 1994, Serial No. PCT / US95 / 02126, filed, Feb. 17, 1995, Ser. No. 08 / 537,874, filed Oct. 30, 1995, Ser. No. 08 / 621,859, filed Mar. 25, 1996, Ser. No. 08 / 621,430, filed Mar. 25, 1996, and Ser. No. 08 / 425,684, filed Apr. 18, 1995, the specifications of which are herein incorporated by reference in their entirety for all purposes.BACKGROUND OF THE INVENTION [0002] Metabolic engineering is the manipulation of intermediary metabolism through the use of both classical genetics and genetic engineering techniques. Cellular engineering is generally a more inclusive term referring to the modification of cellular properties. Cameron et al. (Applied Biochem. Biotech. 38:105-140 (1993)) provide a summary of equivalent terms to describe this type of engineering, including “metabolic engineering”, which is most often used in the context of industrial microbiology and bioprocess engineerin...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
Patent Type & Authority Applications(United States)
Login to view more