Isocoumarin compound and preparation method and application thereof

A technology of isocoumarin and compounds, applied in the field of extraction and separation of plant effective components

Inactive Publication Date: 2015-01-21
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present invention separates and obtains an isocoumarin compound with anti-tobac...

Method used

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  • Isocoumarin compound and preparation method and application thereof
  • Isocoumarin compound and preparation method and application thereof
  • Isocoumarin compound and preparation method and application thereof

Examples

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preparation example Construction

[0027] The preparation method of the isocoumarin compound of the present invention is to use the three tendons of dry Lauraceae plant hairy leaves ( Lindera caudata ) as a raw material obtained through extract extraction, organic solvent extraction, MCI decolorization, silica gel column chromatography, and high-pressure liquid chromatography separation steps, specifically:

[0028] A, extraction of extract: the three tendons of Lauraceae plant hairy leaves ( Lindera caudata ) was crushed to 20-40 mesh, ultrasonically extracted 2-5 times with an organic solvent, 30-60 minutes each time, the extracts were combined, filtered, concentrated extracts under reduced pressure, left to stand, filtered to remove sediment, concentrated into extract a;

[0029] B. Organic solvent extraction: Add 1 to 2 times the amount of water by weight to the extract a, extract 3 to 5 times with an organic solvent equal to the volume of water, combine the organic solvent extraction phase, and concentrat...

Embodiment 1

[0044] Take 1.5 kg of dry three-leaved lauraceae plant hairy leaves, coarsely crush to 40 mesh, ultrasonically extract 4 times with 70% acetone, 60 min each time, combine the extracts; filter the extracts, concentrate under reduced pressure to 1 / 2 of the volume 4; Stand still, filter out the precipitate, concentrate into 120g of extract a; add 240g of water to extract a, extract 5 times with chloroform equal to the volume of water, combine the extraction phases, concentrate under reduced pressure into 50g of extract b; extract b is packed with MCI, add 100 g of 80% methanol water to the extract b to dissolve, then put it on the column, elute with 1 to 4 liters of 90% methanol water, collect the eluate, and concentrate under reduced pressure to obtain 30g extract c; extract c is packed with 200 mesh silica gel 160 g column, add 60 g acetone to dissolve in extract c, then add 100 mesh silica gel 60 g to mix the sample, and put on the column after mixing the sample; 20:1, 9:1, ...

Embodiment 2

[0046] Dried 3 kg of lauraceae hairy leaves, coarsely crushed to 20 mesh, ultrasonically extracted 5 times with 100% ethanol for 30 minutes each time, and the extracts were combined; the extracts were filtered and concentrated under reduced pressure to 1 / 4 of the volume; Stand still, filter out the precipitate, concentrate into 240 g of extract a; add 360 g of water to extract a, extract 3 times with ethyl acetate equal to the volume of water, combine the extract phases, and concentrate under reduced pressure to obtain 100 g of extract a. Extract b; extract b is packed with MCI, add 150 g of 80% methanol water to the extract b to dissolve, then put it on the column, elute with 2 to 6 liters of 90% methanol water, collect the eluate, and concentrate under reduced pressure Obtain 60 g of extract c; use 320 g of 200-mesh silica gel to pack column for extract c, add 120 g of acetone to dissolve extract c, then add 120 g of 100-mesh silica gel to mix the sample, and put it on the ...

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Abstract

The invention discloses an isocoumarin compound and a preparation method and an application thereof. The isocoumarin compound is separated from canella tailed spicebush root and leaf (Lindera caudata) and is named caudacoumarin A; the English name is caudacoumarin A; the molecular formula of the caudacoumarin A is C16H18O5; and the caudacoumarin A has the following structure as shown in the specification. The preparation method of the isocoumarin compound comprises the following steps: with canella tailed spicebush root and leaf as a raw material, extractum extraction, organic solvent extraction, MCI decoloration, column chromatography on silica gel, and high-pressure liquid chromatography separation. The application is an application of the isocoumarin compound in preparation of an anti-mosaic virus medicine. Through a test by a half-leaf method, the caudacoumarin A displays good anti-tobacco mosaic virus activity. The isocoumarin compound disclosed by the invention is simple in structure and good in activity, can be used as a lead compound of resisting the tobacco mosaic virus, and has a relatively good application prospect.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of effective plant components, and in particular relates to an isocoumarin compound in the lauraceae plant Santiaojin and its preparation method and application. Background technique [0002] Dai medicine three tendons Undera Caudata , is a plant of the genus Hndera in the family Lauraceae (Aceae), also known as fragrant face leaf, Huangmai mountain pepper, Puxiang root, bud Sanying (Dai name), and is a herbal medicine commonly used by the Dai people in Xishuangbanna, Yunnan. It was born in It is rich in resources in hillsides, shrubs, and sparse forests at an altitude of 700-2300 m, and is recorded in the "Dictionary of Chinese Medicine". It has the effects of hemostasis and granulation, removing wind, dredging blood and relieving pain, connecting bones and tendons. Isocoumarin is the basic structure of some natural products, which are widely distributed in nature with various...

Claims

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Application Information

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IPC IPC(8): C07D311/76A01N43/16A01P1/00
CPCA01N43/16C07D311/76
Inventor 胡秋芬李银科周敏高雪梅杜刚叶艳清杨海英
Owner YUNNAN MINZU UNIV
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