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Tricyclic alkaloid compound and preparation method and application thereof

A technology of alkaloids, compounds, applied in the field of phytochemistry

Active Publication Date: 2019-10-22
YUNNAN ACAD OF TOBACCO AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention has isolated a new tricyclic alkaloid compound from the horsetail lotus collected in Heqing, Yunnan Province. The compound has not been reported so far. It is worth mentioning that the compound has a significant anti-tobacco flower leaf disease virus activity

Method used

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  • Tricyclic alkaloid compound and preparation method and application thereof
  • Tricyclic alkaloid compound and preparation method and application thereof
  • Tricyclic alkaloid compound and preparation method and application thereof

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preparation example Construction

[0025] The preparation method of the tricyclic alkaloid compound of the present invention is based on the horsetail lotus as a raw material, and is subjected to extraction from extract, silica gel column chromatography and high-pressure liquid chromatography separation and purification steps, specifically comprising:

[0026] A. Extraction: crush and sieve the cauliflower lotus, reflux and extract with 95% ethanol for 2-3 times, each time for 30-50 minutes, combine the extracts and concentrate to obtain material a, add tartaric acid solution to dilute material a to obtain material b , material b is extracted 1 to 3 times with ethyl acetate, the aqueous phase is collected to obtain material c, material c is saturated with sodium carbonate, extracted with ethyl acetate again 1 to 3 times, the extracted ethyl acetate phases are combined, concentrated under reduced pressure to obtain Extract d;

[0027] B. Silica gel column chromatography: Extraction a uses 160-300 mesh silica gel...

Embodiment 1

[0038] Preparation of tricyclic alkaloid compound C 16 h 17 NNaO 3 , including extracting extract, silica gel column chromatography, and high-pressure liquid chromatography separation, specifically adopting the following steps:

[0039] (1) Extraction of extract: Take the whole plant of Sargassum aureus, dry it in the sun, and crush it to about 30-50 meshes. Weigh 2.8-3.5 kg of crushed samples, place them in a 20-L glass reactor, add 10-15 L of 95% ethanol, reflux for 30-50 min, filter out the extract; add 95% 10-15 L of ethanol, reflux extraction for 30-50 min, and filter the extract. The two extracts were combined and concentrated to a small volume, then diluted with 5-8 L of 3% tartaric acid solution, and extracted twice with 5-8 L of ethyl acetate. After the extraction, the aqueous phase was saturated with sodium carbonate, and then extracted twice with 5-8 L of ethyl acetate. The extracted ethyl acetate phases were combined and concentrated under reduced pressure to o...

Embodiment 2

[0045] The sample of horsetail lotus came from Heqing County, Dali, Yunnan. Take the whole plant of Saccharomyces aureus, dry it in the sun, and crush it to about 35 meshes. Weigh 3.0 kg of the pulverized sample, place it in a 20 L glass reactor, add 12 L of 95% ethanol, reflux for extraction for 40 min, and filter out the extract; add 12 L of 95% ethanol to the residue again, and reflux for extraction After 40 min, the extract was filtered off. The two extracts were combined and concentrated to a small volume, then diluted with 6 L of 3% tartaric acid solution, and extracted twice with 6 L of ethyl acetate. After the extraction, the aqueous phase was saturated with sodium carbonate, extracted twice again with 6 L of ethyl acetate, and the extracted ethyl acetate phases were combined and concentrated under reduced pressure to obtain 36.3 g of alkaloid part extract. The extract was mixed with 45 g (80-100 mesh) crude silica gel, dried, and 180 g silica gel (150-200 mesh) was ...

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Abstract

The invention discloses a tricyclic alkaloid compound and a preparation method and application thereof. The tricyclic alkaloid compound is separated from Thalictrum glandulosissimum and named in English as 5,6-dihydro-8,9-dimethoxy-2-methyl-11H-pyrrolo (2,1-b)[3]benzazepin-11-one, has a molecular formula of C16H17NO3, is reddish brown glue, and has the following structural formula shown in the specification. The preparation method comprises the steps of extract extraction, silica gel column chromatography and high pressure liquid chromatography separation and purification. The application is the application of the tricyclic alkaloid compound in the preparation of bacteriostats or the application of the tricyclic alkaloid compound in the preparation of anti-tobacco mosaic virus drugs.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a tricyclic alkaloid compound and its preparation method and application. Background technique [0002] Thalictrum L. (Latin scientific name: Thalictrum L.) is a genus in the Ranunculaceae. This genus is a perennial herb. There are about 200 species in the world, which are widely distributed in Asia, Europe, Africa, North America and South America. Among them, there are about 67 species in China, which are distributed in all provinces and regions of the country, and most of them are distributed in the southwest. About 29 species of plants in this genus are available for medicinal purposes, and some species have the effects of clearing away heat, curing dampness, sweating, stopping dysentery, and treating red eyes. Golden horsetail lotus (scientific name: Thalictrum glandulosissimum (Finet & Gagnep.) W. T. Wang & S. H. Wang), is a herbaceous plant of the genus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/04A01P1/00
CPCC07D487/04
Inventor 孔光辉吴玉萍师君丽李薇胡秋芬杨光宇李晶宋春满刘欣黄海涛李雪梅
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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