Chromone derivative from Cassia pumila and preparation method and application thereof

A kind of technology of stipitate lentils and derivatives, applied in the field of phytochemistry

Active Publication Date: 2019-04-05
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention has isolated a kind of chromone derivative with anti-tobacco mosaic virus activity from P.

Method used

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  • Chromone derivative from Cassia pumila and preparation method and application thereof
  • Chromone derivative from Cassia pumila and preparation method and application thereof
  • Chromone derivative from Cassia pumila and preparation method and application thereof

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preparation example Construction

[0026] The preparation method of the chromone derivative 8-hydroxyl-2-(3-hydroxypropyl)-5-(isobutyryl)-4H-4-chromone of the present invention comprises crushing, extracting extract, silica gel column chromatography , high pressure liquid chromatography and gel column chromatography steps, specifically comprising:

[0027] A, extraction of extract: get the whole plant of lentil lentils, lentil lentils can be crushed or cut into small sections, and use high-concentration methanol (w%: 80% ~ 100%) or high-concentration ethanol (w%: 80%) %~100%) or high-concentration acetone (w%: 70%~100%) is the extraction solvent, extraction solvent: three tendons of hairy leaves (weight ratio)=2~4:1, soak for 24h~72h, extract 3~5 times, the extracts were combined, filtered and concentrated into an extract;

[0028]B. Silica gel column chromatography: the extract is dissolved with pure methanol or pure ethanol or pure acetone with a weight ratio of 1.5 to 3 times, and then mixed with 80 to 100 ...

Embodiment 1

[0034] The lentil lentils sample comes from Xinping County, Yunnan. 2.0 kg of the lentil lentils sample was crushed and extracted 5 times with 95% methanol for 24 hours each time. The extracts were combined, filtered, and concentrated under reduced pressure to obtain an extract. 106g. The extractant is dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 120 g of 80 mesh thick silica gel, and 0.7 kg of 160 mesh silica gel is packed for silica gel column chromatography, and the volume ratio is 20:1, 9:1, 8:2, 7:3, 6:4, 5:5 chloroform-methanol gradient elution, TLC monitoring and merging the same fractions to obtain 6 fractions, among which the chloroform-methanol elution fraction with a volume ratio of 9:1 Separation by Shimadzu's LC-8A semi-preparative high-performance liquid chromatography, with 58% methanol as the mobile phase, Venusil MP C 18 (20×250mm, 5μm) The preparative column is a stationary phase, the flow rate is 20ml / min, the detection w...

Embodiment 2

[0036] The sample of Shandenshan lentils comes from Shuangjiang County, Yunnan Province. Take 4.0kg of Bandenshan lentils and chop them up, extract them with 95% ethanol for 4 times, each time for 48 hours, combine the extracts, filter, and concentrate under reduced pressure to form an extract. Obtain extractum 196g. After dissolving the extract with 2.0 times the amount of pure methanol by weight, mix the sample with 200g of 100-mesh silica gel, pack 1kg of 200-mesh silica gel into a column for silica gel column chromatography, and use a volume ratio of 20:1, 9:1, 8 :2, 7:3, 6:4, 5:5 chloroform-methanol gradient elution, TLC monitoring and merging the same parts to obtain 6 parts, wherein the chloroform-acetone elution part with a volume ratio of 9:1 was used Shimadzu's LC-8A semi-preparative high-performance liquid chromatography separation, with 58% methanol as the mobile phase, Venusil MP C 18 (20×250mm, 5μm) The preparative column is a stationary phase, the flow rate is ...

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Abstract

The invention discloses a chromone derivative from Cassia pumila and a preparation method and application thereof. The chromone derivative is isolated from a whole plant of Cassia pumila and has a molecular formula of C16H16O6 and a compound name of 8-hydroxy-2-(3-hydroxypropyl)-5-(isobutyryl)-4H-4-chromone. The preparation method of the chromone derivative includes: crushing the whole plant of Cassia pumila, extracting, carrying out silica gel column chromatography, and carrying out high-pressure liquid chromatographic separation. Biological activity test on the chromone derivative herein shows that the chromone derivative can well inhibit tobacco mosaic virus. The chromone derivative is easy to isolate, has good activity and may act as a pilot compound to resist tobacco mosaic virus.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a chromone derivative extracted and separated from lentil lentils and its preparation method and application. Background technique [0002] Cassia pumila Lam. is a perennial subshrub-like scattered herb of the genus Cassia in the family Leguminosae. It is also known as Mimosa cassia, cucumber fragrance, dream grass, tart sand, thin bar wood, and sand Grass, cypress grass, saponins, lignified base, 25-75 cm high, much branched, linear sickle-shaped, with small protuberances at the top, branches, branches, petioles, and leaf rachis sparsely pilose, 8-9 Flowering in month; fruiting in October-December. Phyllostachys lenticans grows in bushes or grasses in mountains and open areas. It is mainly distributed in Guangdong, Yunnan and other provinces in China. It is also distributed in India, Indochina Peninsula, Malaysia, and Australia abroad. As a traditional Chines...

Claims

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Application Information

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IPC IPC(8): C07D311/22A01N43/16A01P1/00
CPCA01N43/16C07D311/22
Inventor 李银科黄茹李雪梅杨光宇黄海涛杨叶昆李晶刘欣徐永张建铎
Owner CHINA TOBACCO YUNNAN IND
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