Chromone derivative, preparation method and application thereof
A technology of derivatives, chromones, applied in the field of phytochemistry
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0027] The preparation method of the chromone derivative 6-(2,2-dimethyl-2H-chromene)-2-isopropyl-8-methoxy-4H-4-chromone of the present invention comprises crushing, dipping Cream extraction, silica gel column chromatography, high pressure liquid chromatography and gel column chromatography steps, specifically include:
[0028]A, extract extract: get the Cassia brevifolia plant of whole plant, and Cassia brevifolia can be pulverized or cut into small sections, with high-concentration methanol (w%: 85%~100%) or high-concentration ethanol (w%: 85%) %~100%) or high-concentration acetone (w%: 80%~100%) is the extraction solvent, extraction solvent: three tendons of hairy leaves (weight ratio)=2~4:1, soak for 24h~72h, extract 3~5 times, the extracts were combined, filtered and concentrated into an extract;
[0029] B. Silica gel column chromatography: the extract is dissolved with pure methanol or pure ethanol or pure acetone with a weight ratio of 1.5 to 3 times, and then mixed ...
Embodiment 1
[0035] The sample of Cassia brevifolia comes from Xishuangbanna, Yunnan. Crush 3.0 kg of the sample of Cassia brevifolia and extract it 4 times with 95% methanol for 48 hours each time. The extracts are combined, filtered, and concentrated under reduced pressure to obtain an extract 152 g . The extractant is dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 180 g of 80 mesh thick silica gel, and 0.75 kg of 160 mesh silica gel is packed for silica gel column chromatography, and the volume ratio is 20:1, 9:1, 8:2, 7:3, 6:4, 5:5 chloroform-methanol gradient elution, TLC monitoring and merging the same fractions to obtain 6 fractions, among which the chloroform-methanol elution fraction with a volume ratio of 9:1 Use Agilent 1200 preparative high-performance liquid chromatography to separate, with 68% methanol as mobile phase, Zorbax SB-C18 (21.2 * 250mm, 5 μ m) preparative column is stationary phase, flow velocity is 20ml / min, and the detection wave...
Embodiment 2
[0037] The sample of Cassia brevifolia comes from Mangshi, Yunnan. 2.0 kg of Cassia brevifolia sample is chopped and extracted 5 times with 95% ethanol for 24 hours each time. The extracts are combined, filtered, and concentrated under reduced pressure to obtain an extract. Extract 91g. The extractant is dissolved in pure methanol with a weight ratio of 2.0 times, and then the sample is mixed with 100 g of 100 mesh thick silica gel, and 0.5 kg of 200 mesh silica gel is packed for silica gel column chromatography, and the volume ratio is 20:1, 9:1, 8:2, 7:3, 6:4, 5:5 chloroform-methanol gradient elution, TLC monitoring and merging the same fractions to obtain 6 fractions, among which the volume ratio of 9:1 chloroform-acetone elution fraction Use Agilent 1200 preparative high-performance liquid chromatography to separate, with 68% methanol as mobile phase, Zorbax SB-C18 (21.2 * 250mm, 5 μ m) preparative column is stationary phase, flow velocity is 20ml / min, and the detection wa...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com