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Butyrolactone lignan compound and preparation method and application thereof

A technology of lignans and compounds, applied in the field of butyrolactone lignans and their preparation and application

Inactive Publication Date: 2015-01-07
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention separates a butyrolactone lignan with significant anti-tobacco mosaic virus activity from the solid fermentation product of the Penicillium cladoides group; the compound has not been reported so far

Method used

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  • Butyrolactone lignan compound and preparation method and application thereof
  • Butyrolactone lignan compound and preparation method and application thereof
  • Butyrolactone lignan compound and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The Penicillium clade Penicillium group ( P. ramigena, YNCA0361) bacterial strain was inoculated on the potato dextrose agar medium at room temperature, cultivated for 7 days at 28 degrees Celsius, inoculated in 250 milliliters of conical flasks, each conical flask contained 100 milliliters of potato dextrose medium, and placed in 28 degrees Celsius for shaking culture for 5 day (180 rpm). The large-scale fermentation takes place in 200 500ml vonbach bottles, each containing 100g of rice and 120ml of distilled water. Each bottle was inoculated with 5.0 milliliters of bacteria-containing nutrient medium, and cultured at 25 degrees Celsius for 45 days. Extract by cold soaking with acetone with a volume concentration of 70% for 4 times, each time for 60 minutes, and combine the extracts; filter the extracts, concentrate the extracts under reduced pressure to 1 / 4 ~ 1 / 2 volume, let stand, filter out the precipitate, and concentrate Obtain 215g of extract a; extract a uses ...

Embodiment 2

[0070] The Penicillium clade Penicillium group ( P. ramigena, YNCA0361) strains were inoculated on potato dextrose agar medium at room temperature, and cultured at 28 degrees Celsius for 7 days. Inoculate in a 100 ml Erlenmeyer flask, each Erlenmeyer flask contains 50 ml of potato dextrose medium, and place it at 28°C for 5 days with shaking (180 rpm). Large-scale fermentation was carried out in 100 250ml vonbach bottles, each containing 50g of rice and 60ml of distilled water. Each bottle was inoculated with 5.0 milliliters of bacteria-containing nutrient medium, and cultured at 25 degrees Celsius for 45 days.

[0071] Extract by cold soaking with 99% ethanol for 4 times, each time for 30 minutes, and combine the extracts; filter the extracts, concentrate the extracts under reduced pressure to 1 / 4 ~ 1 / 2 volume, let stand, filter out the precipitate, and concentrate Obtain 125g of extract a; extract a uses 500g of 200 mesh silica gel to pack the column, and the volume ratio ...

Embodiment 3

[0073] The Penicillium clade Penicillium group ( P. ramigena, YNCA0361) strains were inoculated on potato dextrose agar medium at room temperature, and cultured at 28 degrees Celsius for 7 days. Penicillium was inoculated in 250 ml Erlenmeyer flasks, each Erlenmeyer flask contained 100 ml of potato dextrose medium, and placed at 28 degrees Celsius for 5 days (180 rpm). Large-scale fermentations were carried out in 500 250ml vonbach bottles, each containing 50g of rice and 60ml of distilled water. Each bottle was inoculated with 5.0 milliliters of bacteria-containing nutrient medium, and cultured at 25 degrees Celsius for 45 days. Extract by cold immersion in methanol with a volume concentration of 90% for 4 times, each time for 50 minutes, and combine the extracts; filter the extracts, concentrate the extracts under reduced pressure to 1 / 4 ~ 1 / 2 volume, let stand, filter out the precipitate, and concentrate Obtain 400g of extract a; extract a uses 200 mesh silica gel 2000g t...

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Abstract

The invention discloses a butyrolactone lignan compound and a preparation method and an application thereof. The butyrolactone lignan is prepared from a P. ramigena, YNCA0361 strain by steps of fermentation, extraction, chromatography and purification. The molecular formula is C23H22O8 and the butyrolactone lignan compound has a structural formula shown in the specification, wherein the compound is named versicolatcone A. The preservation number of the P. ramigena, YNCA0361 strain is CGMCCNo.4824, and the preservation date is May 2, 2011. The preparation method comprises the following steps: by using fermented solids of the P. ramigena, YNCA0361 strain as raw material, carrying out organic solvent extraction; carrying out column chromatography on silica gel; carrying out MCI decoloring; carrying out reversed-phase column chromatography; carrying out high pressure liquid chromatography and the like. The butyrolactone lignan is applied to preparation of anti-tobacco mosaic virus drugs. The compound disclosed by the invention is novel in structure and good in activity, can be used as a lead compound for resisting the tobacco mosaic virus, and has a relatively good application prospect.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of secondary metabolites from microbial fermentation, and specifically relates to a novel butyrolactone lignan compound with a 2-carbonyl-propyl segment, a preparation method and application thereof. Background technique [0002] There are many types of microbial metabolites, and the products produced in the logarithmic growth phase of the bacteria, such as amino acids, nucleotides, proteins, nucleic acids, sugars, etc., are necessary for the growth and reproduction of the bacteria. These products are called primary metabolites; in the stationary phase of bacterial growth, some bacteria can synthesize some products with specific functions, such as antibiotics, alkaloids, bacterial toxins, plant growth factors, etc. These products have no obvious relationship with the growth and reproduction of bacteria, and are called secondary metabolites. Secondary metabolites are mostly sma...

Claims

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Application Information

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IPC IPC(8): C07D307/68C12P17/04A01N43/08A01P1/00C12R1/80
CPCA01N43/08C07D307/68C12P17/04
Inventor 周敏高雪梅胡秋芬杨海英杜刚李银科叶艳清
Owner YUNNAN MINZU UNIV
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