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Phenolic compound, and preparation method and application thereof

A kind of phenolic compound and compound technology, applied in the field of tobacco chemistry

Active Publication Date: 2014-01-22
YUNNAN RES INST OF TOBACCO SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention isolates a new phenolic compound with anti-tobacco mosaic virus activity from tobacco rhizomes, which has not been reported so far

Method used

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  • Phenolic compound, and preparation method and application thereof
  • Phenolic compound, and preparation method and application thereof
  • Phenolic compound, and preparation method and application thereof

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preparation example Construction

[0023] Phenolic compound C of the present invention 15 h 20 NaO 3 The preparation method comprises extract extraction, silica gel column chromatography, high-pressure liquid chromatography separation steps, specifically comprises:

[0024] A. Extraction of extract: Take tobacco rhizome samples, grind them to 20-40 mesh, use 90-99% ethanol to ultrasonically extract 3-5 times, each time for 30-60 min, combine extracts, filter, and concentrate under reduced pressure to obtain extract ;

[0025] B. Silica gel column chromatography: the extract is packed with 160~200 mesh silica gel dry method with a weight ratio of 2~3 times for silica gel column chromatography; chloroform-acetone solution with a volume ratio of 1:0~1:2 Perform gradient elution, pool the same fractions, collect and concentrate the eluate from each fraction;

[0026] C. High-pressure liquid chromatography separation: the 9:1 part of the eluent in step B is further separated and purified by high-pressure liquid ...

Embodiment 1

[0035]The tobacco rhizome samples were from Yuxi, Yunnan, and the variety was Yunyan-85. Sampling 2.2 kg of tobacco rhizomes was crushed to 30 mesh, extracted 3 times with 95% ethanol and ultrasonically for 30 minutes each time, the extracts were combined, filtered, and concentrated under reduced pressure to obtain an extract, 105.4 g of extract was obtained. The extract was dissolved in pure methanol with a weight ratio of 2.5 times, and then mixed with 150 g of 100-mesh crude silica gel, and 1.5 kg of 160-mesh silica gel was packed into a column for silica gel column chromatography, and the volume ratio was 1:0, 20:1 , 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 9 The chloroform-acetone elution part of :1 is separated by Agilent 1100 semi-preparative high performance liquid chromatography, with 65% methanol as mobile phase, Zorbax SB-C18 (21.2 ' 250 mm, 5 mm) prepar...

Embodiment 2

[0037] The tobacco rhizome samples were from Yuxi, Yunnan, and the variety was Yunyan-85. Sampling 3.5 kg of tobacco rhizomes was crushed to 40 mesh, extracted 5 times with 90% ethanol by ultrasonic, 45 min each time, the extracts were combined, filtered, concentrated under reduced pressure to obtain an extract, and 215 g of extract was obtained. The extract was dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 300 g of 80-mesh crude silica gel, and 1.5 kg of 200-mesh silica gel was packed into a column for silica gel column chromatography, and the volume ratio was 1:0, 20:1 , 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 9 The chloroform-acetone elution part of :1 is separated by Agilent 1100 semi-preparative high performance liquid chromatography, with 65% methanol as mobile phase, Zorbax SB-C18 (21.2 ' 250 mm, 5 mm) preparative column a...

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Abstract

The invention discloses a phenolic compound, and a preparation method and application thereof. The phenolic compound is separated from tobacco roots and stems, and has a molecular formula of C15H20NaO3 and the structure shown in the description. The tobacco roots and stems are taken as raw materials, and the preparation method for the phenolic compound comprises the steps of extract extraction, silica-gel column chromatography and high-pressure liquid chromatographic analysis, and specifically comprises the following steps of performing ethanol ultrasonic extraction after the tobacco roots and stems are crushed, mixing and filtering an extracting solution, and concentrating the extracting solution into extract under reduced pressure; filling the extract into a column for silica-gel column chromatography by using a silica gel dry method; performing gradient elution according to a chloroform-acetone solution in a volume ratio of (1:0)-(1:2); further performing high-pressure liquid chromatographic analysis and purification on the 9:1 part of eluent to obtain the phenolic compound. Tests show that the phenolic compound has higher cell activity to tobacco mosaic viruses; the compound is simple in structure, easy to artificially synthesize and high in activity, and can be used for resisting the tobacco mosaic viruses as a pilot compound.

Description

technical field [0001] The invention belongs to the field of tobacco chemistry, and in particular relates to a phenolic compound extracted from tobacco rhizomes, a preparation method and application thereof. Background technique [0002] Tobacco is one of the most abundant chemical substances among the various plants known to mankind. After decades of research, more than 3,000 individual chemical substances have been identified from tobacco, and there are still many components that have not yet been identified. identified. In addition to being mainly used for smoking cigarettes, tobacco can also extract a variety of valuable chemical components from it, and discover leading compounds that are valuable for development and utilization. In particular, the roots and stems of tobacco are generally treated as waste from the tobacco leaf production process. Therefore, in addition to being consumed as cigarettes, it is of great significance to strengthen the comprehensive utilizat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/58A01N43/16A01P1/00
CPCA01N43/16C07D311/58
Inventor 杨光宇陈永宽刘志华韩熠段沅杏张霞张涛缪明明
Owner YUNNAN RES INST OF TOBACCO SCI
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