The invention discloses a 3, 5-dinitrosalicylic acid hydrazide enzyme-linked immunodetection test kit which comprises a box body, specific antibody solution, 3, 5-dinitrosalicylic acid hydrazine standard solution, an ELISA plate, sample diluent, washing fluid, second antibody solution, colored solution A, colored solution B and stopping solution; wherein, the specific antibody is a rabbit anti-3,5-dinitrosalicylic acid hydrazide antibody; and half antigens and ovalbumin conjugates are coated inside the hole of the ELISA plate; and the second antibody solution is horseradish peroxidase labeled goat anti-rabbit antibody diluent. After the sample to be tested is hydrolyzed by hydrochloric acid, derived by o-nitrobenzaldehyde, extracted by ethyl acetate, re-dissolved by the sample diluent and degreased by n-hexane, and the like, the test kit is used for detection and analysis. The test kit is strong in specificity, and the minimum detection limit is 74.8ppt, thereby being suitable for rapid detection of 3, 5-dinitrosalicylic acid hydrazine in feed, meat, urine, milk and other samples by the health, quality supervision and customs departments, meat processing plants and livestock farms.