60results about How to "Fast pre-processing" patented technology

The invention relates to a method for detecting oligosaccharides in breast milk. The method includes a step of breast milk sample pretreatment, namely taking a breast milk sample, centrifugally removing upper fat, taking lower liquid, adding alcohol solvent, freezing for 5-15min at a temperature ranging from -40 DEG C to -100 DEG C, centrifuging and taking supernatant. The invention further provides a method for qualitative and quantitative detection of oligosaccharides in the pretreated breast milk according to a liquid chromatography-mass spectrometry method. The method for simultaneous detection of various oligosaccharides in non-reducing breast milk by liquid chromatography-mass spectrometry is established and has advantages of effectiveness, quickness and simplicity in pretreatment. AQ Exactive detector is adopted for mass spectrometry, liquid-phase gradient elution parameters and mass spectrometric detection parameters are determined, ion information of non-reducing breast milkoligosaccharides in mass spectrometric detection under a positive ion mode is given, and the method can be well applied to qualitative and quantitative detection of the oligosaccharides in the breastmilk.

The invention discloses a method for detecting the residues of 202 pesticides in tobacco leaves, comprising the following steps of: extracting a tobacco powder sample by an organic solution; purifying a QuEChERS reagent bag; centrifuging; and then utilizing a gas-phase triple quadrupole mass spectrum provided with a procedure temperature-rising sample feeding opening to detect the residues of the pesticides in the tobacco leaves. The method disclosed by the invention has the advantages of less interference, wide covering range of pesticide varieties, simplicity and convenience for pre-treatment, solvent conservation, accuracy and flexibility and the like.

The invention discloses a method for preparing granular hexamethylol melamine, which includes the following steps that: measured water and paraformaldehyde are poured into a reaction kettle, a moderate amount of inorganic base is added to regulate the pH value to 7 to 8, measured melamine is then added after 5 minutes of stirring, and the temperature is then increased to 40 DEG C to 50 DEG C, and is kept for about 15 minutes to 2 hours; a moderate amount of inorganic base is used to regulate the pH value to 8 to 10, and the temperature is then increased to 50 DEG C to 80 DEG C, kept for about 15 minutes to 2 hours and reduced to less than 40 DEG C; acid is used for regulating the pH value to neutral, centrifugal separation is carried out, so that waste water is separated, and after drying, the finished product is obtained; and the separated waste water can be recycled to replace part of water. The invention has the advantages of little pollution and easy operation, moreover, the synthesized hexamethylol melamine is sand-like granules, and can be easily separated and dried, and the hexamethylol melamine has good effect when being used in fields such as the synthesization of glue for rubber, the synthesization of amino resin for baked steel finish and waterproof coating for mineral wool boards.

The invention relates to a method for detecting the content of boric acid and borate in cosmetics. A method for measuring boric acid and borate in cosmetics by microwave digestion -ICP-OES comprises the following steps of: performing microwave digestion on a cosmetic sample through nitric acid and hydrogen peroxide to decompose organic substances, guiding sample solution into an inductive coupling plasma atomic emission spectrometer atomizer to form aerosol, loading the aerosol into high-temperature high-frequency plasma by argon to generate ionization and generate a characteristic spectral line, and measuring the spectral line intensity of a boron element and an internal standard yttrium element in the sample solution, wherein the intensity of the boron element is in direct proportion to the content of the boron element; and correcting a response value of the boron element according to the ratio of an actually measured yttrium internal standard response value to an expected internal standard response value, performing quantitative measurement by adopting a standard curve method, calculating the content of the boron element in the cosmetics, and obtaining the content of the boric acid and the borate through conversion coefficients. The method has the characteristics of strong specificity, high sensitivity and accurate result.

The invention relates to a method for pre-treatment of wine, the wine is a sample which is waiting for being detected for content of pesticide residues, and the method comprises three steps of extraction, purification and concentration. The invention also relates to a pesticide residues detection method for the wine, and the pre-treatment method performs pre-treatment on the wine sample. The pre-treatment method can realize rapid pre-treatment on the wine sample for detecting the pesticide residues, and has the advantages of less step, short time consumption, no requirement of massive organic solvent, environmental protection and low cost.

The invention relates to a method for detecting the content of soluble zinc salt in cosmetic, in particular to a method for detecting the content of soluble zinc salt in cosmetic by an ultrasound centrifugal-inductively coupled plasma atomic emission spectrometry detection technology, which comprises the following steps: extracting cosmetic samples through ultrasound centrifugal, introducing the sample solution into an inductively coupled plasma atomic emission spectrometer nebulizer to form aerosol, loading the aerosol into a high-temperature high-frequency plasma by argon, causing ionization, generating characteristic lines, detecting line intensity of zinc in the sample solution, wherein the intensity and content of zinc are directly proportional, correcting response value of zinc according to the specific value of an actual-measurement indium internal standard response value and a predicted indium internal standard response value, adopting a calibration curve method for quantitative detection, and calculating the content (calculating by zinc) of the soluble zinc salt in the cosmetic. The invention has the characteristics of strong specificity, high sensitivity and correct result.

The invention relates to the technical field of cosmetic detection, in particular to a method for detecting cannabidiol in cosmetics. The detection method comprises the following steps: mixing a sample with an extraction solvent, and performing ultrasonic extraction to obtain an extracting solution; adding the extracting solution into a purification column for purification, and filtering to obtaina target detection solution, wherein the purification column is an MPFC-QuEChERS high-fat ultrafiltration type purification column made of a nano composite material; analyzing and detecting a targetdetection liquid by adopting an ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometry method. The detection method realizes the detection of cannabidiol in cosmetics,the analysis time is short, and qualitative and quantitative detection results are stable and reliable.

The invention relates to a rapid analysis method for determining formaldehyde in electronic cigarette liquid. The method comprises the following steps that firstly, a dispersant is added to an electronic cigarette liquid sample, then a DNPH derivatization reagent and an acid solution are added to perform derivatization reaction, an alkaline reagent and an internal standard substance are added, and finally the formaldehyde content in the electronic cigarette liquid is determined through LC-MS / MS analysis. The method is high in sensitivity, strong in anti-interference performance and good in repeatability, the pretreatment process is simple and rapid, and the method is suitable for detection of electronic cigarette liquid samples on a large scale.

The invention discloses a method for carrying out chromatographic test pretreatment on a lithium battery electrolyte. A sodium carbonate solution with the purity being 99.9% is prepared into a saturated sodium carbonate water solution; the saturated sodium carbonate water solution is taken and put in a centrifugal tube, and an electrolyte sample is added for vortex oscillation; an organic solvent is added, vortex oscillation continues, standing is carried out, and after the mixture is completely layered, supernatant liquid is taken out and put in a glass sample bottle; the operation is repeated two times; finally, the glass sample bottle is subjected to nitrogen blowing at low temperature, and after the organic solvent is removed, liquid in the glass sample bottle is 3 ml to 10 ml. After chromatographic test pretreatment is carried out on the lithium battery electrolyte, qualitative and quantitative errors caused by the low content of an additive are eliminated, the lithium salt removal effect is good, therefore, consumption of gas chromatograph chromatographic columns and liner tubes is reduced, and the testing cost is reduced.

The invention discloses a method for rapidly identifying illegally added poppy shells in herbal tea. The method comprises the following steps: (1) carrying out ultrasonic treatment on a liquid herbal tea sample to obtain a herbal tea sample, regulating the herbal tea sample to be slightly alkaline, adding an organic solvent and sodium chloride, carrying out vortex extraction, centrifuging, layering, adding an ammonium acetate solution into supernatant, and uniformly mixing, thereby obtaining to-be-tested liquid; or adding an organic solvent into a solid herbal tea sample, carrying out ultrasonic treatment, cooling an extracting solution to room temperature, making up to constant volume, adding an ammonium acetate solution into the extracting solution, and uniformly mixing, thereby obtaining to-be-tested liquid; and (2) filtering the to-be-tested liquid by virtue of an organic phase filter membrane, with an acetic acid-methanol solution and an ammonium acetate solution as a mobile phase, carrying out reversed phase chromatographic column separation, and detecting five main ingredients, namely morphine, codeine, thebaine, papaverine and narcotine, in the poppy shells on eluent by adopting an ultra-high performance liquid chromatography-tandom mass spectrometer. The method disclosed by the invention has the advantages that pretreatment is simple and rapid, qualitative and quantitative treatment is accurate, and the illegally added poppy shell ingredients in the herbal tea can be rapidly and reliably detected.

The invention provides a method for detecting the hydrolysate of residual antibiotic, and is characterized in that the method comprises the following steps that: a dairy product to be detected and a blank control non-antibiotic milk sample undergo sterilization and cooling, and then ferment is added into the mixture so as to measure the initial pH value of the fermentation broth of the mixture; then, the mixture undergoes heat preservation at a temperature of between 40 and 45 DEG C for 1 hour, and the pH value of the fermentation broth is measured so as to calculate the difference value between the variation in pH value before and after fermentation and the variation value of non-antibiotic milk contrast sample; when the difference value is more than or equal to 0.3, the sample is judged as a positive sample. The minimum concentration of enzymolysis product detected by the method is equal to 10ppb; therefore, the method can realize sensitive and accurate detection of antibiotic enzymolysis residue in dairy products.

The invention discloses a method for measuring chlorobenzene organic pollutants in leather by gas chromatography-mass spectrometry. The method comprises the following steps of preparing a to-be-measured sample, extracting a sample, confecting a standard working solution and detecting a to-be-measured sample solution. According to the method, the ultrasonic extraction-gas chromatography-mass spectrometry is combined; the chlorobenzene organic pollutants in the leather can be measured at the same time; the method has the advantages of accurateness, quickness and high sensitivity; the reliable detection method support is provided for the quick detection of the leather.

The invention relates to a method for rapid analysis determination of acetaldehyde in an electronic cigarette liquid, wherein the method comprises the following steps: firstly, adding a dispersing agent in an electronic cigarette liquid sample; then adding a DNPH derivatization reagent and an acidic solution, carrying out a derivatization reaction, and then adding an alkaline reagent and an internal standard substance; and finally analyzing and determining the content of acetaldehyde in the electronic cigarette liquid by using LC-MS / MS. The method has the advantages of high sensitivity, strong interference resistance, good repeatability, and simple and fast pretreatment process, and is suitable for detection of a large number of electronic cigarette liquid samples.

The invention discloses a method for rapidly detecting the content of B vitamins in pig intestinal contents / excrement. The method comprises the following steps: weighing cecum contents / excrement of pigs as a sample, adding a methanol / acetonitrile / water solution, and performing homogeneous extraction to obtain a sample solution; and performing UPLC-MS / MS-MRM detection analysis on the sample solution to finally obtain the content of eight B vitamins in the sample, wherein the eight B vitamins are vitamin B1, vitamin B2, nicotinic acid, nicotinamide, pyridoxal, pyridoxine, vitamin B9 and vitaminB12. According to the method, an MRM technology is utilized, an ultra-high performance liquid chromatography system and a mass spectrometer are combined, and the content of a plurality of B vitamins in the samples such as the pig intestinal contents and the excrement can be rapidly detected based on targeted metabonomics of an MRM method.

The invention provides a analysis and detection method of gas free radical in the mainstream smoke of cigarette, characterized by: using MALDI-FT-ICR-MS to detect the gas radical in mainstream smoke of cigarettes, concrete steps include: 1) choosing radical trap agent; 2) trapping gas radical in the gas; 3) choosing radical character; 4) Pointing and entering MALDI-FT-ICR-MS analysis; 5) analysis of spectrum analytical data; 6) further checking out the cigarettes free radical and its adducts in mainstream smoke of cigarettes. Advantages ars: MALDI-MS FT-ICR-soft ionization technology, high-resolution and high sensitivity can provide accurate analysis of the molecular mass, elemental composition, molecular structure and experience of information, to distinguish between two compounds in the mixture of very molecular weight nearly, is qualitative specific, sensitive, rapid and convenient detection advantage, but also suitable for high-throughput experiments. It provides a very favorable analytical tool for radical research and development.

The present invention provides a method for rapid separation determination of nicotine in water. According to the method, a sample is subjected to centrifugation filtration, is separated by using a liquid chromatography column, and is eluted through a methanol-water system, electrospray tandem triple quadrupole mass spectrometry is adopted to monitor possibly-appearing nicotine fragments, and nicotine in water is rapidly separated and determined within 6 min. The method has characteristics of low detection limit, high accuracy, easy operation, and rapidness, is applicable for investigation and evidence collection works of tobacco counterfeiting, and has wide application and promotion values.

The invention discloses a 3, 5-dinitrosalicylic acid hydrazide enzyme-linked immunodetection test kit which comprises a box body, specific antibody solution, 3, 5-dinitrosalicylic acid hydrazine standard solution, an ELISA plate, sample diluent, washing fluid, second antibody solution, colored solution A, colored solution B and stopping solution; wherein, the specific antibody is a rabbit anti-3,5-dinitrosalicylic acid hydrazide antibody; and half antigens and ovalbumin conjugates are coated inside the hole of the ELISA plate; and the second antibody solution is horseradish peroxidase labeled goat anti-rabbit antibody diluent. After the sample to be tested is hydrolyzed by hydrochloric acid, derived by o-nitrobenzaldehyde, extracted by ethyl acetate, re-dissolved by the sample diluent and degreased by n-hexane, and the like, the test kit is used for detection and analysis. The test kit is strong in specificity, and the minimum detection limit is 74.8ppt, thereby being suitable for rapid detection of 3, 5-dinitrosalicylic acid hydrazine in feed, meat, urine, milk and other samples by the health, quality supervision and customs departments, meat processing plants and livestock farms.

The invention discloses a liquid chromatography-mass spectrometry detection method for polyphenols. The method comprises: ultrasonically extracting a to-be-tested sample to obtain a to-be-tested extract; and performing a liquid chromatography-mass spectrometry test on the to-be-tested extract to determine the content of the polyphenols in the to-be-tested sample. The method can be used to detect the content of polyphenols in the to-be-tested sample simply, quickly and accurately.

The invention discloses a method for rapidly analyzing the occurrence state of rare earth by using visible light-near infrared reflectance spectroscopy, which comprises the following steps: (1) drying a weathering crust sample, putting the weathering crust sample into a vessel, and flattening the surface; (2) carrying out reflection spectrum measurement on the sample in the vessel by using a visible light-near infrared reflection spectrometer, wherein the spectrum range is set to be 350-2500nm; measuring the powder sample for a plurality of times from different directions at an interval of 90 degrees, measuring the rock block sample for a plurality of times from different directions at an interval of 30 degrees, and recording original spectral data; and (3) processing the obtained original spectrum data, carrying out peak shape fitting on 800nm absorption band characteristics, obtaining the position and relative intensity information of an absorption band, and judging the occurrence state of the rare earth element. The method provided by the invention can meet the requirement of weathering crust ion adsorption type rare earth deposit prospecting exploration work on rapid sample analysis.

The invention relates to a detecting method of a growth regulator, in particular to a method for detecting a growth regulator in a foliar fertilizer. According to the method for detecting the growth regulator in the foliar fertilizer, a series of growth regulator solutions with known concentrations are prepared from growth regulator standard substances and subjected to high-performance liquid chromatography analysis, the concentrations of the plant growth regulator are taken as horizontal coordinates, peak areas are taken as vertical coordinates, and an linear regression equation of the growth regulator concentrations and the peak areas is obtained; a sample to be tested is treated, the treated sample is subjected to high-performance liquid chromatography analysis, and a high-performance liquid chromatogram and a peak area of the growth regulator are obtained, the peak area is substituted into the linear regression equation, and the corresponding growth regulator concentration is obtained. The method has high universality, good selectivity and high sensitivity and operability and is applicable to high-performance liquid chromatography determination of forchlorfenuron, uniconazole, naphthylacetic acid and abscisic acid in the foliar fertilizer.

The present invention discloses a pretreatment method of benzo (a) pyrene collected by glass fiber filter paper, first, glass fiber filter paper with the collected benzo (a) pyrene is cut, and put into a brown headspace sample bottle, then the headspace sample bottle is continued to be added with methanol as a desorption solvent, and then added with a certain amount of alumina, the alumina can absorb and purify interference impurities in a sample; the headspace sample bottle is sealed, and put into an ultrasound device with circulating cooling water for ultrasound desorption for a period of time; finally the headspace sample bottle is taken out, and put into a centrifuge for centrifuging for a period of time at a set rotational speed, and a supernatant is taken and expanded and analyzed by chromatography. The method has the advantages of simple and available appliances, closed pretreatment process, small outside world pollution, fast pretreatment speed, high benzo (a) pyrene desorption recycling efficiency and the like.

The invention relates to a phase chromatography tandem mass spectrum measuring method for 4-(methyl nitrosamino)-1-(3-pyridyl)-1-butanone(NNK) and N-nitrosonornicotine (NNN) in tobacco, and belongs tothe technical field of tobacco chemistry analysis. A methanol solution containing 0.5% of acetic acid is used for extracting NNK and NNN in a tobacco sample, and after the extracting solution is filtered, UPC2-MS / MS is used for analyzing and quantifying the concentration of NNK and NNN in the extracting solution. The sample treating process is simple and quick, and batched strict work analysis isconvenient. When the phase chromatography tandem mass spectrum is used for detection, the used main moving phase is supercutical fluid CO2, a small amount of methyl alcohol is used as a cosolvent, and the environmentally friendly advantage is achieved. According to the method, the detection limit of NNK is 0.05 ng / mL, the quantitation limit is 0.18 ng / mL, the detection limit of NNN is 0.04 ng / mL,and the quantitation limit is 0.12 ng / mL. The method is high in sensitivity and good in repeatability and is applied to synchronous measurement of NNK and NNN in the tobacco sample.

The invention discloses a kit for detecting valproic acid drug concentration in dry blood spots. The kit comprises a kit body, a first container, a calibrator card and a quality control card, whereinthe calibrator card is a blood spot collection card fixed with a group of valproic acid dry blood spot samples as dry blood spot calibration points, and the number of the dry blood spot calibration points is at least six, the quality control material card is a blood spot collection card fixed with at least one group of valproic acid dry blood spot samples as dry blood spot quality control points,the number of each group of dry blood spot quality control points is at least three, the first container is filled with an internal standard extracting solution, and an internal standard adopts isotopic substituted valproic acid. The invention further discloses a detection method of the kit. The kit provided by the invention has advantages of simple preparation of reagents, less required amount ofsamples, convenience in storage and transportation, rapid pretreatment, short analysis period, lower cost and the like, and is suitable for popularization in clinical conventional detection.

The invention discloses an ion chromatography post-column derivatization kit for detecting ammonia nitrogen content in a sample and a detection method. The detection method is combined with the kit and has the advantages of simple and rapid pretreatment, low detection limit, strong anti-interference capability and simple operation. The specific method comprises the following steps of firstly filtering a to-be-detected sample aqueous solution, then feeding the solution into a sample, separating and eluting through a cation exchange chromatographic column, heating and reacting with salicylate and hypochlorous acid ions under an alkaline condition in the presence of sodium nitroferricyanide to generate a blue compound, and enabling the product to have relatively strong absorption under a certain wavelength. Therefore, the ammonia nitrogen content is detected.

The invention relates to a method for rapid analysis determination of propyl aldehyde in an electronic cigarette liquid, wherein the method comprises the following steps: firstly, adding a dispersing agent into an electronic cigarette liquid sample; then adding a DNPH derivatization reagent and an acid solution, carrying out a derivatization reaction, and then adding an alkaline reagent and an internal standard substance; and finally analyzing and determining the content of propyl aldehyde in the electronic cigarette liquid by LC-MS / MS. The method has the advantages of high sensitivity, strong interference resistance, good repeatability, and simple and fast pretreatment process, and is suitable for detection of a large number of electronic cigarette liquid samples.

The invention discloses a method for detecting residual quantity of disulfuron and metabolite thereof in plant-derived food, wherein the method comprises the steps: after oscillating and extracting a plant-derived food sample, carrying out dispersive solid-phase extraction and purification by taking a titanium carbide multilayer nanosheet as a purifying agent; and determining the residual quantity of the disulfuron and the metabolite thereof in the sample by adopting ultra-high performance liquid chromatography-tandem mass spectrometry. By optimizing a sample pretreatment method and instrument detection conditions, the pretreatment process is simple and rapid, the operation is accurate, the sensitivity is high, and the repeatability is good; and the sample recovery rate can reach 102.9%, and the relative standard deviation (RSD) is less than 6.0%.

The invention discloses a method for simultaneously measuring chlorotoluene compounds in leather by gas chromatography-mass spectrography. The method comprises the following steps: preparing a sample to be measured; extracting the sample; preparing a standard working solution; detecting a sample solution to be measured. According to the method, ultrasonic extracting, gas chromatography and mass spectrography are combined, so that chlorotoluene organic pollutants in the leather can be measured simultaneously; the method has the advantages of accuracy, quickness and high sensitivity; reliable detection method support is provided for quick detection of the leather.

The invention discloses a microorganism single-cell metabonomics analysis method based on MALDI mass spectrometry. The method specifically comprises the following steps: arraying the surface of a conductive slide, centrifugally washing a microbe cell suspension in double distilled water, carrying out gradient dilution after microbe washing, dropwise adding the diluted cell liquid to a specified position of the arrayed slide and drying, carrying out MALDI mass spectrometry operation and the like. The method overcomes the defects of complex pretreatment of microbial single cells, poor repeatability between data points and inaccurate data acquisition image positioning at present, and has the advantages of simple steps, high background repeatability, accurate positioning, high data acquisition speed and high sensitivity.

The invention discloses a Raman rapid detection method for sibutramine in weight-reducing tea. The method comprises the following operation steps of S1, preparing a sample, namely taking a proper amount of weight-reducing tea solid, adding an aqueous solution of which the mass volume is 5-50 times of the mass volume of the weight-reducing tea solid, and carrying out vortex oscillation for 1 min orultrasonic extraction for about 3 min to obtain a weight-reducing tea liquid; S2, pre-treating, namely centrifuging the weight-reducing tea liquid obtained in the step S1 until the weight-reducing tea liquid is clarified, taking 1 volume of supernatant, adding 0.5-2 volumes of ethyl acetate or similar organic solvents, carrying out oscillation extraction, and carrying out standing layering; taking out an organic phase, adding GCB according to a ratio of 0.01-0.05 g / mL, oscillating, taking out the organic phase, adding 0.5-2 volumes of 1mM-1M acid liquor, carrying out back extraction, and taking the water phase for detection; and S3, detecting, namely adding the water-phase sample to be detected in S2 into a Raman detection tube, adding metal nanoparticles and a coagulant, and uniformly mixing to carry out Raman detection. According to the method, the sibutramine in the weight-reducing tea can be quickly and sensitively identified, the pretreatment is simple, a reagent is cheap, the detection mode is quick, and the method has wide application space in the field of quick food detection.