Method for rapidly identifying illegally added poppy shells in herbal tea
A technology of illegally adding poppy husks, which is applied in the field of identification of poppy husks, can solve the problems of endangering consumers' health and addiction, achieve better extraction effect, less interference, and improve the effect of chromatographic peak shape
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Embodiment 1
[0062] The method for quickly identifying the illegal addition of poppy shells in herbal teas provided by this example comprises the following steps:
[0063] (1) Take about 50 mL of a bulk herbal tea sample sold on the market and place it in a 250 mL beaker, and extract it ultrasonically at 40°C for 20 minutes at a frequency of 40khz to obtain a uniform sample;
[0064] (2) Take out and cool to room temperature, accurately weigh 5g (accurate to 0.01g) of uniform liquid herbal tea sample, put it in a 50mL plastic centrifuge tube, add 5mL of ammonia solution or sodium hydroxide solution with a pH of 8-9, and then add Analytical pure methanol 10.0mL, add sodium chloride 5g, Vortex with MS3 digital vortex mixer at 2000r / min for 3 minutes, centrifuge at 8000r / min for 5 minutes, separate layers, use a pipette to accurately draw 1.0mL of supernatant into the nitrogen blowpipe, and then accurately add 1.0mL of 10mmol / L ammonium acetate solution (pH=4.5), so that the final volume i...
Embodiment approach
[0098] (1) For solid herbal tea samples, weigh 1g (accurate to 0.01g) of the solid sample and place it in a 50mL volumetric flask, add 40mL of methanol to cover the bottle, place it in an ultrasonic cleaner, adjust the temperature to 50°C and ultrasonically at a fixed frequency of 40khz After 25 minutes, the main components of the poppy shell were fully extracted, cooled to room temperature, and the volume was adjusted to 50 mL with methanol, and 1.0 mL of 10 mmol / L ammonium acetate solution (pH=4.5) was added to a nitrogen blowing pipe to make the final volume 2.0 mL, and vortex Rotate and mix evenly to prepare the solution to be tested;
[0099] (2) Use a 1mL disposable syringe to draw 1mL of the test solution, filter it through a 0.45μm pore size organic phase filter, put it in the sample bottle, cover it, and wait for the test; if the content of the test substance in the test solution exceeds the linearity of the standard curve range, it can be appropriately diluted with a...
Embodiment 3
[0114] Effect of positive herbal tea samples not subjected to the heating shaking test on the results.
[0115] (1) Weigh 5g of liquid herbal tea sample (accurate to 0.01g), put it in a 50mL plastic centrifuge tube, add 5mL of ammonia solution or sodium hydroxide solution with a pH of 8-9, and then add analytically pure methanol (or acetonitrile) 10.0mL, add 5g of sodium chloride, vortex for 3 minutes, centrifuge at 8000 rpm for 5 minutes, separate layers, use a pipette to accurately draw 1.0mL of the supernatant into the nitrogen blowing pipe, and then accurately add 1.0mL10mmol / L ammonium acetate solution (pH=4.5), make the final volume to 2.0 mL, vortex and mix evenly to prepare the solution to be tested; the remaining steps are performed as in Example 1.
[0116] In positive sample 1, the contents of morphine, papaverine, narcotine, codeine, and thebaine were 264.2 μg / kg, 87.5 μg / kg, and 144.1 μg / kg, respectively, not detected, and 139.91 μg / kg; positive sample In 2, the...
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