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Method for rapidly identifying illegally added poppy shells in herbal tea

A technology of illegally adding poppy husks, which is applied in the field of identification of poppy husks, can solve the problems of endangering consumers' health and addiction, achieve better extraction effect, less interference, and improve the effect of chromatographic peak shape

Active Publication Date: 2017-08-29
GUANGDONG TESTING INST OF PROD QUALITY SUPERVISION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drinking herbal tea illegally added with poppy husk powder or liquid obtained by boiling poppy husks is likely to make people who have never been exposed to drugs or who are highly sensitive to drugs addicted, seriously endangering the health of consumers
[0003] At present, there are no national standards and related documents for rapid identification of illegally added opium poppy husks in herbal tea, and a reliable detection method needs to be established

Method used

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  • Method for rapidly identifying illegally added poppy shells in herbal tea
  • Method for rapidly identifying illegally added poppy shells in herbal tea
  • Method for rapidly identifying illegally added poppy shells in herbal tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The method for quickly identifying the illegal addition of poppy shells in herbal teas provided by this example comprises the following steps:

[0063] (1) Take about 50 mL of a bulk herbal tea sample sold on the market and place it in a 250 mL beaker, and extract it ultrasonically at 40°C for 20 minutes at a frequency of 40khz to obtain a uniform sample;

[0064] (2) Take out and cool to room temperature, accurately weigh 5g (accurate to 0.01g) of uniform liquid herbal tea sample, put it in a 50mL plastic centrifuge tube, add 5mL of ammonia solution or sodium hydroxide solution with a pH of 8-9, and then add Analytical pure methanol 10.0mL, add sodium chloride 5g, Vortex with MS3 digital vortex mixer at 2000r / min for 3 minutes, centrifuge at 8000r / min for 5 minutes, separate layers, use a pipette to accurately draw 1.0mL of supernatant into the nitrogen blowpipe, and then accurately add 1.0mL of 10mmol / L ammonium acetate solution (pH=4.5), so that the final volume i...

Embodiment approach

[0098] (1) For solid herbal tea samples, weigh 1g (accurate to 0.01g) of the solid sample and place it in a 50mL volumetric flask, add 40mL of methanol to cover the bottle, place it in an ultrasonic cleaner, adjust the temperature to 50°C and ultrasonically at a fixed frequency of 40khz After 25 minutes, the main components of the poppy shell were fully extracted, cooled to room temperature, and the volume was adjusted to 50 mL with methanol, and 1.0 mL of 10 mmol / L ammonium acetate solution (pH=4.5) was added to a nitrogen blowing pipe to make the final volume 2.0 mL, and vortex Rotate and mix evenly to prepare the solution to be tested;

[0099] (2) Use a 1mL disposable syringe to draw 1mL of the test solution, filter it through a 0.45μm pore size organic phase filter, put it in the sample bottle, cover it, and wait for the test; if the content of the test substance in the test solution exceeds the linearity of the standard curve range, it can be appropriately diluted with a...

Embodiment 3

[0114] Effect of positive herbal tea samples not subjected to the heating shaking test on the results.

[0115] (1) Weigh 5g of liquid herbal tea sample (accurate to 0.01g), put it in a 50mL plastic centrifuge tube, add 5mL of ammonia solution or sodium hydroxide solution with a pH of 8-9, and then add analytically pure methanol (or acetonitrile) 10.0mL, add 5g of sodium chloride, vortex for 3 minutes, centrifuge at 8000 rpm for 5 minutes, separate layers, use a pipette to accurately draw 1.0mL of the supernatant into the nitrogen blowing pipe, and then accurately add 1.0mL10mmol / L ammonium acetate solution (pH=4.5), make the final volume to 2.0 mL, vortex and mix evenly to prepare the solution to be tested; the remaining steps are performed as in Example 1.

[0116] In positive sample 1, the contents of morphine, papaverine, narcotine, codeine, and thebaine were 264.2 μg / kg, 87.5 μg / kg, and 144.1 μg / kg, respectively, not detected, and 139.91 μg / kg; positive sample In 2, the...

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Abstract

The invention discloses a method for rapidly identifying illegally added poppy shells in herbal tea. The method comprises the following steps: (1) carrying out ultrasonic treatment on a liquid herbal tea sample to obtain a herbal tea sample, regulating the herbal tea sample to be slightly alkaline, adding an organic solvent and sodium chloride, carrying out vortex extraction, centrifuging, layering, adding an ammonium acetate solution into supernatant, and uniformly mixing, thereby obtaining to-be-tested liquid; or adding an organic solvent into a solid herbal tea sample, carrying out ultrasonic treatment, cooling an extracting solution to room temperature, making up to constant volume, adding an ammonium acetate solution into the extracting solution, and uniformly mixing, thereby obtaining to-be-tested liquid; and (2) filtering the to-be-tested liquid by virtue of an organic phase filter membrane, with an acetic acid-methanol solution and an ammonium acetate solution as a mobile phase, carrying out reversed phase chromatographic column separation, and detecting five main ingredients, namely morphine, codeine, thebaine, papaverine and narcotine, in the poppy shells on eluent by adopting an ultra-high performance liquid chromatography-tandom mass spectrometer. The method disclosed by the invention has the advantages that pretreatment is simple and rapid, qualitative and quantitative treatment is accurate, and the illegally added poppy shell ingredients in the herbal tea can be rapidly and reliably detected.

Description

technical field [0001] The invention belongs to the technical field of identification of poppy husks, in particular to a method for rapidly identifying illegally added poppy husks in herbal tea. Background technique [0002] Poppy husk, commonly known as smoke husk, has certain medicinal value and can be used for pain relief, sedation and cough relief. Its main components are alkaloids such as morphine, codeine, thebaine, papaverine and narcotine. Illegally adding poppy husks to herbal tea can increase the cough-relieving effect, but long-term consumption is addictive, and when the dosage is large, it will also cause irritability, excitement, and convulsions in patients. Poppy shell is a legal drug, and it is a controlled substance officially announced by the state to be included in the list of narcotics. In order to enhance the cough-relieving effect of herbal tea, criminals attract more customers and obtain greater profits. Drinking herbal tea illegally added with poppy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李锦清綦艳周勇李聪刘仲义陈满英
Owner GUANGDONG TESTING INST OF PROD QUALITY SUPERVISION
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