59results about How to "Sensitive recognition" patented technology

Disclosed is a preparation method of a fluorescent sensor for detecting microRNA. The preparation method comprises the following steps: preparing polyacrylic acid modified GdF3:Tb3+ nanoparticles through a solvothermal method; preparing a solution of GdF3:Tb3+ nanoparticles coupled to a single oligonucleotide chain probe I through catalytic condensation of EDC and NHS; preparing a fresh solution of colloidal gold nanoparticles through reducing chloroauric acid with sodium citrate; coupling a single oligonucleotide chain probe II to the gold nanoparticles by utilizing combination of sulfydryl groups and gold; and preparing a PBS solution of the fluorescent sensor for detecting microRNA. The invention has advantages that: the preparation method of the fluorescent sensor is simple, and is safe to operate, mild in condition, and low in cost; artificially synthesized hsa-miR-122-5p in a buffer solution can be sensitively and specifically recognized; and the fluorescent sensor has a linear range of 1fM-100pM, and a detection limit of 0.5fM.

The invention discloses an Arduino-based multi-device interactive display control system and method. The system comprises a control server, wherein the control server receives a data display request command from a control subsystem, and the data display request command comprises card data or knob data; corresponding data is acquired from a database according to the data display request command, the acquired data is sent to a display subsystem, and the control server controls the display subsystem to select a corresponding device for data display; and the database is maintained through a background management subsystem. The system provided by the invention has the beneficial effects that different display devices can be selected according to different display contents, so that the display contents can be presented to a user in a most suitable and perfect manner, and the user experience can be improved.

The invention belongs to the technical fields of a functional nanometer material and a fluorescence probe material, and particularly relates to a surface functionalized Rhodamine B modified gold nanoparticle probe capable of performing visual detection on Dursban and a hydrolysis product thereof, and a preparation method of the probe. The novel detection probe is a combined product of monodispersed nanoparticles and Rhodamine B. The probe is simple in synthesis method, low in detection limit, easily available in raw material and remarkable in visualization; and during detection, the fluorescence intensity is in direct proportion to the concentration of the product, the color recovers to be orange and trace pesticide residues can be detected. Fluorescence and ultraviolet visible absorptionresearch results indicate that the probe can serve as a novel probe for detecting the pesticide Dursban and the hydrolysis product thereof.

The invention relates to the field of bactericide detection technologies, in particular to a method of detecting bactericide residues in food through a Raman enhanced spectrum. The method comprises the steps that cut-up samples are taken and placed into an extraction solvent, an extracting solution is obtained after ultrasonic extraction, and the ratio of the samples to the extraction solvent is 1g:5ml; after an extracting agent is added into the extracting solution for oscillating extraction, standing is performed for layering, sodium sulfate is added after extracted supernatant is taken, standing is performed for layering after oscillation, and supernatant is taken and placed in a volatilizing bottle to volatilize to be dry, wherein the volume ratio of the extracting solution to the extracting agent is 1:1; oscillation is performed after an organic solvent is added into the volatilizing bottle, and a sample to-be-detected solution is obtained; and the sample to-be-detected solution,sol-type nano-gold and an aggregating agent are sequentially added into an SERS tube, and a Raman spectrograph is used for detection after uniform mixing. Through the method, difenoconazole, tebuconazole, pyrimethanil and other bactericides in the food can be identified quickly and sensitively, the pretreatment procedure is simple, and therefore the method has broad application space in the food rapid detection field.

The invention relates to an MMC (Modular Multilevel Converter) DC transmission line protection method based on voltage correlation, which belongs to the technical field of power system relay protection. When a fault occurs in a DC transmission line, the positive and negative voltages at both ends of the line are collected and a correlation coefficient is obtained. Then, whether the fault is a single-pole grounding fault is judged according to the correlation coefficient. If the fault is judged to be a non-single-pole grounding fault, the inter-pole voltage is calculated, and the change rate ofthe inter-pole voltage is obtained. Finally, whether there is a short circuit between the two poles is judged according to the change rate of the voltage. The method of the invention has strong transition resistance endurance, and the data window is only 3ms. The method is quick in operation, and has a strong application prospect.

The invention relates to an MMC direct current power transmission line fault recognition method based on correlation, and belongs to the technical field of relay protection of a power system. The method comprises the steps of firstly reading a fault voltage and fault current data acquired by a high-speed collection device of a measurement terminal; then performing differential calculation on the acquired voltage data, and analyzing correlation between the original fault voltage and the fault current to acquire a correlation coefficient kui, performing correlation analysis on the original faultcurrent to acquire a correlation coefficient kuti; when the correlation coefficient between the original fault voltage and the current the absolute value of kui is less than 0.15, judging that the fault is a single pole ground fault; when kui is not more than -0.15, and the correlation coefficient between a voltage change rate and the original fault current kuti is more than 0, judging that the fault is a dual pole short circuit ground fault; when kui is not more than -0.15 and kuti is less than 0, judging that the fault is an alternating current side fault of an inverter station; and when kui is not less than 0.15, judging that the fault is an alternating current side fault of a rectification station.

The invention relates to a MMC direct current transmission line fault identification method based on SOD transformation, belonging to the technical field of power system relay protection. The method comprises a step of reading fault voltage and fault current data acquired by a high-speed acquisition device of a measuring end, a step of performing cross-order differential SOD transformation on theobtained voltage and current data to obtain Su(n) and Si(n), a step of multiplying Su(n) by Si(n) to obtain Sp(n) and taking a minimum value Spmin from Sp(n) for fault judgment, a step of judging an out-of-zone fault when Spmin is larger than -20, a step of judging a positive pole ground fault when Spmin is larger than -450and is small than -20, and a step of judging a bipolar short-circuit faultwhen Spmin is smaller than -450. According to the method, the single-ended voltage and current data is used, then the voltage and current data is subjected to differential calculation and SOD transformation, a bipolar ground fault, the bipolar short-circuit fault and the out-of-zone fault are judged, the communication with an opposite end signal is needed, and the faults can be reliably and sensitively identified.

The invention provides aspartic acid modified gold nanoparticles and a preparation method and application thereof. Aspartic acid, as a reducing agent and a stabilizing agent, reacts with aurichlorohydric acid so as to prepare gold nano; and the synthesized functionalized gold nano can be used for identifying specificity of trivalent chromium ions. Functionalized modification on the surface of thegold nano is realized without complex ligand exchange. The method is simple, can identify specificity of trivalent chromium ions by eyes through interaction of the trivalent chromium ions and the synthesized functionalized gold nano, thereby having a good application prospect in the environment analysis chemical field.

The invention discloses an iron ion detection probe based on fluorescence enhancement as well as a preparation method and an application of the probe. The structure of the fluorescent probe is shown in formula I, and the probe is formed after 5-thioaldehyde acetal salicylaldehyde as a raw material and hydrazine are subjected to a condensation reaction to form salicylaldehyde azine. The probe has stable optical performance, high detection sensitivity to iron ions, low lower limit of detection and has the limit of detection of 2.35 nM and response range of 0.08-1 mu M. The probe has good selectivity and has no response to cations such as barium ions, calcium ions, lithium ions, magnesium ions, ammonium ions, nickel ions, zinc ions, mercury ions, cobalt ions, lead ions and lead ions. The probe is simple in synthesis, mild in condition and high in yield. The fluorescent molecular probe has actual application value in fields of detection of iron ions in biochemistry and environmental chemistry.

The invention discloses an NMDAR recombinant protein related to autoimmune encephalitis as well as a coding sequence, a preparation method and application of the NMDAR recombinant protein, and belongs to the technical field of biology. The coding sequence of the NMDAR recombinant protein related to the autoimmune encephalitis is as shown in SEQ ID NO. 1; and the amino acid sequence of the recombinant protein is as shown in SEQ ID NO. 2. The preparation method of the recombinant protein comprises the following steps of connecting a coding sequence as shown in SEQ ID NO.1 to a recombinant vector to construct a recombinant protein expression vector; transforming into competent cells of escherichia coli, and culturing to obtain a recombinant protein expression strain; extracting plasmids, and transfecting the plasmids into CHO-S cells for culture; and collecting a cell supernatant, and purifying to obtain the recombinant protein. The invention also provides the application of the recombinant protein in preparation of an anti-NMDAR autoantibody detection material, and an anti-NMDAR autoantibody detection kit. The NMDAR recombinant protein can be recognized by an anti-NMDAR autoantibody in the blood of a patient through heterologous recombinant expression of NR1-ATD, and is expressed in vitro in quantity, so that the production cost is reduced, and quantitative detection can be realized.

The invention relates to a visual rapid detection mask for exhaled air coronavirus. The visual rapid detection mask comprises a mask main body, a breather valve and a test strip, wherein the test strip comprises a filter disc, and a sample pad, a combination pad, a color development pad and a water absorption pad which are in lap joint in sequence; the combination pad comprises colloidal gold coated with a coronavirus N protein antibody; and the color development pad is provided with a detection line and a quality control line, the detection line is coated with a coronavirus E protein antibody, and the quality control line is coated with an anti-coronavirus N protein antibody IgG. Rapid and accurate detection of the coronavirus can be achieved within 0.5-1 h when a subject wears the mask. The mask takes exhaled air as a detection object, and the exhaled air carries more viruses compared with body fluid such as sputum and saliva, so that by utilizing the mask, suspected patients which cannot be diagnosed temporarily and other people (susceptible people, old people, children and the like) which are inconvenient to go to hospitals for detection can be quickly and preliminarily diagnosed.

The invention discloses an intelligent fitness system which comprises: a fitness device used for performing fitness exercises; the data application model which is used for generating a motion data interface and/or a virtual reality scene according to the external data so as to output and display; the data acquisition device which is used for acquiring exercise parameters in fitness exercises and sending the exercise parameters to the data application model in real time so as to generate an exercise data interface and/or a virtual reality scene related to the fitness exercises; the display device which is used for displaying the motion data interface and/or the virtual reality scene in real time; working parameters of the fitness device can be collected in the fitness process, data integration is conducted through a program carried by a mobile terminal or a PC, a motion data interface or a virtual reality scene is generated to be displayed, real-time data dynamic display fitness or immersive fitness of the virtual scene can be achieved, and the fitness experience feeling of a user is improved; the system has multiple fitness functions; the instrument balance weight can achieve intelligent induction and is sensitive in recognition and high in accuracy.

The invention relates to a full-automatic capacitance monitoring machine. The machine comprises a feeding unit, a detection unit and a discharging unit which are arranged in a linear longitudinal sequence, wherein the detection unit comprises an upper and lower surface appearance detection mechanism, a pin height detection mechanism and a side surface appearance detection mechanism which are arranged in a longitudinal sequence, a feeding conveying belt is arranged on the side, close to the detection unit, of the feeding unit, a blocking and discharging combined type material distributing mechanism is arranged at one end of the feeding conveying belt, a multi-claw type carrying mechanism is arranged on the side face of the material distributing mechanism and the side face of the detection unit, and the carrying mechanism, the feeding unit, the detection unit and the discharging unit are controlled by a controller to operate. According to the full-automatic capacitance monitoring machine, detection of upper and lower surface appearances, welding pins and side surface appearances of a capacitor is completed through the detection unit, then classified discharging is carried out through the discharging unit, the automation degree of a machine table is high, the detection unit integrates multiple detection into one workbench, an occupied area of the machine table is small, and the production efficiency is greatly improved.