Method of detecting bactericide residues in food through Raman enhanced spectrum

A technology of enhanced spectrum and fungicides, applied in the field of fungicide detection, can solve the problems of cumbersome processing procedures and long detection cycles, and achieve the effect of simple pre-processing procedures and wide application space

Inactive Publication Date: 2019-09-13
河北凌析科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The currently reported analysis methods for fungicide residues include gas chromatography, high performance liquid chromatography, gas (liquid) chromatography-mass spectrometry, etc., but these methods have shortcomings such as cumbersome pretreatment procedures for testing samples and long testing cycles.

Method used

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  • Method of detecting bactericide residues in food through Raman enhanced spectrum
  • Method of detecting bactericide residues in food through Raman enhanced spectrum
  • Method of detecting bactericide residues in food through Raman enhanced spectrum

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1: Raman detection method of difenoconazole in rapeseed

[0038] Step 1, sample pretreatment: take 3 parts of rapeseed, add 0.01mg / kg, 0.03mg / kg and 0.05mg / kg of difenoconazole respectively, take 1g of chopped rapeseed and put it in a beaker, add 5mL Acetonitrile extraction, cover the lid for ultrasonic extraction for 5min. Take 3ml of the extract after ultrasonic extraction in a sample tube, add 3ml of acetone for extraction, shake for 10s, let it stand for stratification, then take 2ml of the extracted upper layer after stratification into a reagent bottle, add sodium sulfate for moisture absorption, shake 10s, let it stand for layering.

[0039] Step 2, prepare the sample solution to be tested: take 1ml of the supernatant in a volatilization bottle, put it in the volatilization pool until it evaporates to dryness, then take 400ul of acetonitrile in the volatilization bottle, shake for 10s, and obtain the sample solution to be tested.

[0040] Step 3, prepa...

Embodiment 2

[0044] Embodiment 2: the Raman detection method of tebuconazole in tomato

[0045] Step 1, sample pretreatment: take 3 parts of tomatoes, spike with 0.01mg / kg, 0.03mg / kg and 0.05mg / kg of tebuconazole respectively, take 1g of shredded tomatoes and put them in a beaker, add 5mL of acetonitrile to extract , cover the lid and conduct ultrasonic extraction for 5 min. Take 3ml of the extract after ultrasonic extraction in a sample tube, add 3ml of acetone for extraction, shake for 20s, let it stand for stratification, then take 2ml of the extracted upper layer after stratification into a reagent bottle, add sodium sulfate for moisture absorption, shake 10s, let it stand for layering.

[0046] Step 2, prepare the sample solution to be tested: take 1ml of the supernatant in a volatilization bottle, put it in the volatilization pool until it evaporates to dryness, then take 400ul of acetonitrile in the volatilization bottle, shake for 20s, and obtain the sample solution to be tested. ...

Embodiment 3

[0051] Embodiment 3: the Raman detection method of pyrimethanil in cucumber

[0052] Step 1, sample pretreatment: take 3 parts of cucumber, spike with 0.01mg / kg, 0.02mg / kg and 0.03mg / kg of pyrimethanil respectively, take 1g of chopped cucumber and put it in a beaker, add 5mL of acetonitrile to extract , cover the lid and conduct ultrasonic extraction for 5 min. Take 3ml of the extract after ultrasonic extraction in a sample tube, add 3ml of acetone for extraction, shake for 15s, let it stand for stratification, then take 2ml of the extracted upper layer after stratification into a reagent bottle, add sodium sulfate for moisture absorption, shake 10s, let it stand for layering.

[0053] Step 2, prepare the sample solution to be tested: take 1ml of the supernatant in a volatilization bottle, put it in a volatilization pool until it evaporates to dryness, then take 400ul of acetonitrile in the volatilization bottle, shake for 15s, and obtain the sample solution to be tested.

...

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Abstract

The invention relates to the field of bactericide detection technologies, in particular to a method of detecting bactericide residues in food through a Raman enhanced spectrum. The method comprises the steps that cut-up samples are taken and placed into an extraction solvent, an extracting solution is obtained after ultrasonic extraction, and the ratio of the samples to the extraction solvent is 1g:5ml; after an extracting agent is added into the extracting solution for oscillating extraction, standing is performed for layering, sodium sulfate is added after extracted supernatant is taken, standing is performed for layering after oscillation, and supernatant is taken and placed in a volatilizing bottle to volatilize to be dry, wherein the volume ratio of the extracting solution to the extracting agent is 1:1; oscillation is performed after an organic solvent is added into the volatilizing bottle, and a sample to-be-detected solution is obtained; and the sample to-be-detected solution,sol-type nano-gold and an aggregating agent are sequentially added into an SERS tube, and a Raman spectrograph is used for detection after uniform mixing. Through the method, difenoconazole, tebuconazole, pyrimethanil and other bactericides in the food can be identified quickly and sensitively, the pretreatment procedure is simple, and therefore the method has broad application space in the food rapid detection field.

Description

technical field [0001] The invention relates to the technical field of bactericide detection, in particular to a method for detecting bactericide residues in food by using Raman enhanced spectroscopy. Background technique [0002] Difenoconazole (difenoconazole) is a triazole fungicide, which has the characteristics of stable chemical properties, low toxicity and broad-spectrum antibacterial properties. It can be transported into the crops and transported to the diseased part to exert its bactericidal effect. It has a good control effect on common diseases such as anthracnose, leaf blight, black rot, and powdery mildew in various fruits and vegetables, especially for vegetables. Leaf spot and pear scab have a good control effect. In addition, it can also stimulate the growth of crops and improve the yield and quality of fruits and vegetables. [0003] Tebuconazole is a light ethyl triazole derivative fungicide, which belongs to the ergosterol synthesis inhibitor. It can be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/34G01N1/38
CPCG01N1/34G01N1/38G01N21/658
Inventor 栗娇娇杨明李媛媛
Owner 河北凌析科技有限公司
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