Rhodamine B modified gold nanoparticle probe capable of performing visual detection on Dursban and hydrolysis product thereof, and preparation method of probe

A gold nanoparticle and hydrolyzate technology, applied in color/spectral property measurement, fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of cumbersome operation, inability to meet real-time online detection, and inconvenient equipment.

Inactive Publication Date: 2018-06-29
ANQING NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, a variety of methods have been used for the detection of pesticides, such as gas, liquid chromatography-mass spectrometry, electrochemical analysis, enzyme-linked immunosorbent assay,

Method used

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  • Rhodamine B modified gold nanoparticle probe capable of performing visual detection on Dursban and hydrolysis product thereof, and preparation method of probe
  • Rhodamine B modified gold nanoparticle probe capable of performing visual detection on Dursban and hydrolysis product thereof, and preparation method of probe
  • Rhodamine B modified gold nanoparticle probe capable of performing visual detection on Dursban and hydrolysis product thereof, and preparation method of probe

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1: Preparation of gold nanoparticles. 50mL of chloroauric acid solution (1mM) was heated to boiling in a round bottom flask equipped with a reflux condenser, and 5mL of 38.8mM trisodium citrate was quickly injected under vigorous stirring, and the reflux was continued for 30 minutes. The color of the solution changed from light yellow to deep red, stop heating, continue to stir and cool to room temperature. Store in the refrigerator at 4°C for later use.

Embodiment 2

[0016] Example 2: Modification of gold nanoparticles. A 2 mM Rhodamine B solution was prepared using ultrapure water. Take 10 μL of rhodamine B solution and stir it into 10 mL of gold nanoparticles with a particle size of 13 nm at a concentration of about 15 nM. The mixed solution was stirred at room temperature for two hours, and the solution was collected after the reaction was complete.

Embodiment 3

[0017] Example 3: Preparation of chlorpyrifos alkaline hydrolyzate diethoxythiophosphoric acid. Acetonitrile and water with a volume ratio of 1.25:1 were added into a one-necked flask, and mixed uniformly under magnetic stirring. Then, 2 mL of diethoxyphosphoryl thiochloride and 1.6 g of sodium hydroxide were added to the mixture, the molar ratio was about 3:1. The reaction was stirred at 30° C. for 12 hours, then the reaction liquid was adjusted to weak acidity with hydrochloric acid, extracted with chloroform, the organic phase was collected, and the product was obtained after rotary evaporation.

[0018] The present invention further characterizes and detects rhodamine B modified gold nanoparticles, and the process is as follows:

[0019] (1) Morphological observation of rhodamine B-modified gold nanoparticles

[0020] The morphology of gold nanoparticles was observed with a Hitachi H7100 transmission electron microscope, and the results are as follows: figure 1 Shown: ...

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Abstract

The invention belongs to the technical fields of a functional nanometer material and a fluorescence probe material, and particularly relates to a surface functionalized Rhodamine B modified gold nanoparticle probe capable of performing visual detection on Dursban and a hydrolysis product thereof, and a preparation method of the probe. The novel detection probe is a combined product of monodispersed nanoparticles and Rhodamine B. The probe is simple in synthesis method, low in detection limit, easily available in raw material and remarkable in visualization; and during detection, the fluorescence intensity is in direct proportion to the concentration of the product, the color recovers to be orange and trace pesticide residues can be detected. Fluorescence and ultraviolet visible absorptionresearch results indicate that the probe can serve as a novel probe for detecting the pesticide Dursban and the hydrolysis product thereof.

Description

technical field [0001] The invention belongs to the technical field of functional nanometer materials and fluorescent probe materials, in particular to a method for preparing surface-functionalized gold nanoparticles. The invented gold nanoparticles are combined with rhodamine B, using the principle of fluorescence resonance energy transfer (FRET), to obtain fluorescence-enhanced pesticide fluorescent probes, which can be used to detect trace pesticide residues. Background technique [0002] Pesticides are a variety of organic toxic compounds, which are widely used in agricultural production due to their defensive effects on insects, bacteria, weeds, nematodes and other species that harm crops. Among them, organophosphorus pesticides are the most widely used, which also causes serious accumulation and residue of organophosphorus pesticides in water resources, soil and crops, endangering environmental safety and human health. The U.S. Environmental Protection Agency (the U.S...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/31
Inventor 王彦石道飞
Owner ANQING NORMAL UNIV
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