Method for detecting content of hyaluronic acid in human serum

A technology for hyaluronic acid and human detection, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of environmental radioactive pollution, long reaction time, and short shelf life of reagents, and achieve environmental pollution, fast response, and long shelf life. long effect

Inactive Publication Date: 2011-06-15
BEIJING NORTH INST OF BIOLOGICAL TECH
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  • Abstract
  • Description
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Problems solved by technology

[0003] But the existence of above-mentioned method is as follows deficiencies 1, use too m...

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  • Method for detecting content of hyaluronic acid in human serum
  • Method for detecting content of hyaluronic acid in human serum

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Embodiment 1

[0008] 1) Preparation of coated plate: HA (HA-BSA) coupled with bovine serum albumin (BSA) was diluted with 0.01M phosphate buffer (PB, pH7.4) in a certain proportion to make coating solution, etc. The volume was added to each well of the microplate and left overnight at 4°C. The coating solution was discarded, an equal volume of blocking solution (0.01M PB, 5-10% sucrose, 0.5-1% BSA, pH7.4) was added, and left overnight at 4°C. Discard the blocking solution, dry it naturally, and vacuum-seal it in an aluminum foil bag for long-term storage.

[0009] 2) Detection operation process:

[0010] a. Add an equal volume of HA calibrator or sample to be tested to each well of the microplate.

[0011] b. Add an equal volume of HABP-Eu3+ to each well of the microplate.

[0012] c. Put the microplate on the shaker and shake it at room temperature for 60 minutes.

[0013] d. Suck out or pour out the reaction solution, add washing solution to wash 6 times, and pat the plate dry after t...

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Abstract

The invention relates to a method for detecting hyaluronic acid in human serum, body fluid or tissue homogenate by a radioimmunoassay method. By a method of using solid phase-coated and europium-marked hyaluronic acid binding protein (HABP-Eu<3+>), reagents such as a first antibody, a second antibody and the like are not needed, a sample is easy to add, reaction is quick, the reagents have long shelf life and are environmental-friendly, europium is stably combined with HABP, storage time can be more than 12 months, and radioactive pollution is avoided.

Description

technical field [0001] The invention relates to a method for detecting hyaluronic acid (Hyaluronic Acid, HA for short) in human serum, body fluid or tissue homogenate by radioimmunoassay method. Background technique [0002] As disclosed in document CN 1047391A, this document discloses a sensitive, accurate and rapid method for detecting hyaluronic acid (HA) content in serum, body fluid or tissue homogenate. It adds quantitative hyaluronic acid-binding protein (HABP) to the unknown sample, then adds iodine-labeled hyaluronic acid to combine with the remaining HABP in the sample reaction, and then uses the first antibody and the second antibody to precipitate HABP and measure After its radioactivity, its concentration is calibrated. The detection of serum HA is helpful for the diagnosis and differential diagnosis of liver diseases, condition judgment and prognosis evaluation. The method and its kit also provide the necessary method and material basis for scientific research...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/545
Inventor 吕东川
Owner BEIJING NORTH INST OF BIOLOGICAL TECH
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