Iron ion detection probe based on fluorescence enhancement as well as preparation method and application of probe

A detection probe and luminescence enhancement technology, applied in the field of chemical analysis and detection, can solve problems such as lack of fluorescent probes, and achieve the effects of sensitive identification, low detection limit and strong specificity

Active Publication Date: 2019-01-18
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the current development of iron ion probes is concerned, there is still a lack of fluorescent probes that can overcome solubility and specificity that can be truly applied in practice, especially probes that can work in water environments.

Method used

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  • Iron ion detection probe based on fluorescence enhancement as well as preparation method and application of probe
  • Iron ion detection probe based on fluorescence enhancement as well as preparation method and application of probe
  • Iron ion detection probe based on fluorescence enhancement as well as preparation method and application of probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Synthesis of fluorescent probes of the present invention

[0038] The preparation route of fluorescent probe of the present invention is as follows:

[0039]

[0040] Get compound 1, the hydrazine hydrate that mass fraction is 80% is dissolved in dehydrated alcohol, and the ratio of the amount of described compound 1 and hydrazine hydrate substance is 2:1,60 o Stirring under the condition of C for 2 hours, the solid was completely precipitated, filtered, and the crude product was recrystallized from absolute ethanol to obtain compound I in the form of yellow needles; that is, the iron ion detection probe represented by formula (I).

[0041] , Pale yellow solid, yield 82%, pale yellow solid. IR (KBr) cm -1 3440, 3138, 1630, 1440, 1264, 976, 760. 1 H NMR (500 MHz, CDCl 3 ) δ 11.21 (s, 1H), 7.78 (s, 2H), 7.36 – 7.18 (m, 4H), 6.90 (d, J = 8.0 Hz, 2H), 5.67 – 5.32 (m, 1H), 5.11(s, 2H), 3.08 – 2.85 (m, 8H), 2.18 – 1.85 (m, 4H). 13 C NMR (125 MHz, CDCl 3 ) δ 15...

Embodiment 2

[0043] Ratiometric Fluorescent Probe Molecular Working Curve

[0044] Take 2 mL of the prepared water-dispersed solution of ratiometric fluorescent probe molecules, add 20 μL of ferric sulfate aqueous solution with different concentrations (0, 0.08, 0.1, 0.2, 0.5, 0.6, 0.8, 1 μmol / L), and after standing for 30 minutes, the fluorescence The spectrometer records the changes of the fluorescence intensity at 553nm respectively. Taking the fluorescence intensity as the ordinate and the iron ion concentration as the abscissa, the working curve of the ratio fluorescent probe molecule is obtained by fitting. In this embodiment, the ratiometric fluorescent probe is used for the determination of iron ion concentration. Under the excitation of 380nm excitation light, the relationship between the fluorescence intensity and the change of iron ion concentration is as follows figure 1 shown.

Embodiment 3

[0046] Fluorescent Probe Molecular Cation Selectivity Determination

[0047] Take 2mL of the water-dispersed solution of the ratio fluorescent probe molecule prepared in Example 2, add 20 μL of Cu with a concentration of 0.01M 2+ , Fe 3+ , Ni 2+ , Hg 2+ , Pb 2+, Co 2+ , NH 4 + , Ba 2+ , Zn 2+ , Li + , Na + , Ca 2+ , Mg 2+ After the aqueous solution was left for 30 minutes, the fluorescence spectrometer recorded the changes of the fluorescence intensity at 553 nm. Experimental results show that, except for iron ions, other particles do not cause obvious changes in fluorescence, indicating that the probe molecules of the present invention have good selectivity. In this embodiment, the molecular selectivity of fluorescent probes is determined. Under the excitation of 380nm excitation light, the relationship between the fluorescence intensity and the concentration of iron ions is as follows figure 2 shown.

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Abstract

The invention discloses an iron ion detection probe based on fluorescence enhancement as well as a preparation method and an application of the probe. The structure of the fluorescent probe is shown in formula I, and the probe is formed after 5-thioaldehyde acetal salicylaldehyde as a raw material and hydrazine are subjected to a condensation reaction to form salicylaldehyde azine. The probe has stable optical performance, high detection sensitivity to iron ions, low lower limit of detection and has the limit of detection of 2.35 nM and response range of 0.08-1 mu M. The probe has good selectivity and has no response to cations such as barium ions, calcium ions, lithium ions, magnesium ions, ammonium ions, nickel ions, zinc ions, mercury ions, cobalt ions, lead ions and lead ions. The probe is simple in synthesis, mild in condition and high in yield. The fluorescent molecular probe has actual application value in fields of detection of iron ions in biochemistry and environmental chemistry.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to an iron ion detection probe based on luminescence enhancement and its preparation method and application. Background technique [0002] Iron is the second most abundant metallic element in the earth's crust and the most abundant trace element in the body. Iron is one of the essential elements in life, and many biochemical processes at the cellular level require the participation of iron ions. Therefore, it is of great significance to study fluorescent molecular probes that can be used for qualitative and quantitative analysis of iron ions. Because iron ions are paramagnetic metal ions, combining with traditional fluorescent probe molecules can easily cause fluorescence quenching, so most of the currently reported iron ion fluorescent molecular probes are quenching, fluorescence-enhancing iron ion probes Molecules are less reported. [0003] With the gra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D339/08C09K11/06G01N21/64
CPCC07D339/08C09K11/06C09K2211/1092G01N21/6428
Inventor 赵云辉罗月阳周智华王涵周航斌谭海龙
Owner HUNAN UNIV OF SCI & TECH
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