Tumor detection kit adopting lamprey Li protein as marker

A detection kit and detection reagent technology, applied in the field of tumor detection kits using lamprey plum protein as a marker, to achieve the effects of sensitive recognition, low production cost and high stability

Inactive Publication Date: 2018-06-15
LIAONING NORMAL UNIVERSITY
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far there is no relevant report on the application of lamprey Li protein (Li protein) as an alternative capture reagent and detection reagent to tumor detection kits

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tumor detection kit adopting lamprey Li protein as marker
  • Tumor detection kit adopting lamprey Li protein as marker
  • Tumor detection kit adopting lamprey Li protein as marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The tumor detection kit using lamprey plum protein as a marker of the present invention, the same as the existing tumor detection kit, has a capture reagent and a detection reagent. The difference from the prior art is that the capture reagent and the detection reagent are both Recombinant Lamprey Li protein that specifically labels sugars and sphingomyelin on tumor cell surface lipids. Lamprey recombinant plum protein that can specifically label sugars and sphingomyelin on the lipids on the surface of tumor cells was prepared as follows:

[0028] (1) Induced expression of lamprey recombinant plum protein rLi protein

[0029] ①Transform BL21(DE3) competent cells with 1 μL pColdⅠ-rLi protein plasmid DNA, and smear the plate;

[0030] ②Select positive clones, inoculate them into 10 mL of LB medium containing Amp+, and culture overnight at 37°C and 120 rpm with shaking;

[0031] ③The next day, inoculate with the above culture solution / LB medium containing Amp+ at a volum...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a tumor detection kit adopting a lamprey Li proteins as a marker. The kit contains a capture reagent and a detection reagent, and the capture reagent and the detection reagentare recombinant lamprey Li proteins capable of specifically marking sugar and sphingomyelin of lipid rafts on the surfaces of tumor cells. The above prepared recombinant protein rLi protein has higherstability, specificity and affinity than antibody molecules, and still keeps complete antigen binding capability and is more sensitive to tumor cell antigens after being stored at 4 DEG C for one year or more when in a dissolved state. The kit has the advantages of high detection sensitivity, convenience in storage and transportation, long validity and low production cost.

Description

technical field [0001] The invention relates to a tumor detection kit, in particular to a tumor detection kit using lamprey plum protein as a marker with high detection sensitivity, convenient storage and transportation, long validity period and low production cost. Background technique [0002] There are many kinds of tumor markers. The common tumor markers are divided into carbohydrate antigens, embryonic antigens, cytokeratin, tumor-related enzymes and hormones, natural self-antigens, etc. Some tumor markers can detect different types of cancer It has a broad spectrum, and some have high sensitivity and specificity for specific types of tumors. For example: alpha-fetoprotein (alpha-fetoprotein, AFP), carcinoembryonic antigen (CEA), pancreatic embryonic antigen (pancreatic oncofetal antigen, POA), carbohydrate antigen (carbohydrate antigen, CA, breast cancer marker CA153 and gastric cancer Markers such as CA724 are now relatively common tumor markers. [0003] Jawless ve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/574
CPCG01N33/533G01N33/57484
Inventor 李庆伟逄越卢佳丽
Owner LIAONING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap