Method for analysing and detecting gas-phas free-radical in cigarette mainstream smoke

Abstract

The invention provides a analysis and detection method of gas free radical in the mainstream smoke of cigarette, characterized by: using MALDI-FT-ICR-MS to detect the gas radical in mainstream smoke of cigarettes, concrete steps include: 1) choosing radical trap agent; 2) trapping gas radical in the gas; 3) choosing radical character; 4) Pointing and entering MALDI-FT-ICR-MS analysis; 5) analysis of spectrum analytical data; 6) further checking out the cigarettes free radical and its adducts in mainstream smoke of cigarettes. Advantages ars: MALDI-MS FT-ICR-soft ionization technology, high-resolution and high sensitivity can provide accurate analysis of the molecular mass, elemental composition, molecular structure and experience of information, to distinguish between two compounds in the mixture of very molecular weight nearly, is qualitative specific, sensitive, rapid and convenient detection advantage, but also suitable for high-throughput experiments. It provides a very favorable analytical tool for radical research and development.

Description

technical field [0001] The invention relates to an analysis and detection method of gaseous phase free radicals in cigarette mainstream smoke, which adopts matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FT-ICR-MS) method, and is applied to detection and Analysis of gas-phase free radicals in mainstream cigarette smoke. Background technique [0002] In recent years, chemically active and low-content free radical compounds have become more and more important in the development of chemistry, environmental science, biology, and toxicology. However, the development of methods for the detection and identification of free radicals has been relatively slow. At present, electron spin resonance (ESR) is mainly used for direct detection and analysis of free radicals with relatively stable activity. For free radical substances with very active chemical properties, it is mainly to use free radical scavenger or free radical...

AI Technical Summary

Problems solved by technology

[0003] However, ESR and ESR-spin trapping technology, as a relatively mature and widely used free radical detection method, also has some shortcomings: it cannot distinguish free radicals or free radical adducts with similar structures in the mixture; the detection and identification of free radicals is limited to Types of free radicals (such as carbon nucleus free radicals, oxygen nucleus free radicals and sulfur nucleus free radicals)

[0004] MALDI technology was initially mainly used for the analysis of macromolecular compounds. However, due to the high sensitivity of MALDI technology, its application in the analysis of small molecular compounds has attracted extensive attention from analytical chemists in recent years. With the continuous emergence of new matrices and It has been successfully applied, and its application research in the analysis of low molecular weight compounds has been reported continuously

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