Liquid chromatography-mass spectrometry detection method for polyphenols
A liquid mass spectrometry and detection method technology, applied in the field of analytical chemistry, can solve the problems of low sensitivity, time-consuming, poor accuracy, etc., and achieve the effects of high detection sensitivity and accuracy, broad application prospects, and good linear relationship
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[0069] In the present embodiment, the polyphenols in garlic are detected by HPLC-MS / MS, and the method is as follows:
[0070] 1. Preparation of the test solution:
[0071] Accurately weigh 100mg of garlic sample into a 2mL centrifuge tube, add 1.5mL of 60% methanol aqueous solution, ultrasonic extraction time is 30min, ultrasonic frequency is 20KHz, ultrasonic power is 450W, ultrasonic temperature is 40℃; Microporous membrane, prepared need testing solution;
[0072] 2. Preparation of standard solution:
[0073] Weigh 10 mg of the standard product, add 10 mL of methanol to dissolve, and prepare a standard solution with a mass concentration of 1000 mg / L, and a mixed standard solution with a mass concentration of 10 mg / L;
[0074] 3. Using HPLC-MS / MS detection conditions
[0075] 1. The chromatographic conditions are as follows:
[0076]The HPLC-MS / MS liquid chromatographic system is a Symbiosis liquid chromatograph from Spark Company in the Netherlands, and the chromatogra...
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