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Liquid chromatography-mass spectrometry detection method for polyphenols

A liquid mass spectrometry and detection method technology, applied in the field of analytical chemistry, can solve the problems of low sensitivity, time-consuming, poor accuracy, etc., and achieve the effects of high detection sensitivity and accuracy, broad application prospects, and good linear relationship

Active Publication Date: 2018-12-11
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Description
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  • Application Information

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Problems solved by technology

In recent years, the analysis method for the content of polyphenolic compounds is mainly high-performance liquid chromatography, which has low sensitivity and poor accuracy.
Moreover, the ingredients of garlic and other items are complex, and the pretreatment process of garlic and other samples by conventional methods is very cumbersome and time-consuming, which is not conducive to the rapid detection and quality control of garlic polyphenols, or uses a large amount of organic solvents, which is not environmentally friendly and harmful. economy
[0003] Therefore, the detection method of the existing polyphenols in food and medicine needs to be improved

Method used

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  • Liquid chromatography-mass spectrometry detection method for polyphenols
  • Liquid chromatography-mass spectrometry detection method for polyphenols
  • Liquid chromatography-mass spectrometry detection method for polyphenols

Examples

Experimental program
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Effect test

Embodiment 1

[0069] In the present embodiment, the polyphenols in garlic are detected by HPLC-MS / MS, and the method is as follows:

[0070] 1. Preparation of the test solution:

[0071] Accurately weigh 100mg of garlic sample into a 2mL centrifuge tube, add 1.5mL of 60% methanol aqueous solution, ultrasonic extraction time is 30min, ultrasonic frequency is 20KHz, ultrasonic power is 450W, ultrasonic temperature is 40℃; Microporous membrane, prepared need testing solution;

[0072] 2. Preparation of standard solution:

[0073] Weigh 10 mg of the standard product, add 10 mL of methanol to dissolve, and prepare a standard solution with a mass concentration of 1000 mg / L, and a mixed standard solution with a mass concentration of 10 mg / L;

[0074] 3. Using HPLC-MS / MS detection conditions

[0075] 1. The chromatographic conditions are as follows:

[0076]The HPLC-MS / MS liquid chromatographic system is a Symbiosis liquid chromatograph from Spark Company in the Netherlands, and the chromatogra...

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Abstract

The invention discloses a liquid chromatography-mass spectrometry detection method for polyphenols. The method comprises: ultrasonically extracting a to-be-tested sample to obtain a to-be-tested extract; and performing a liquid chromatography-mass spectrometry test on the to-be-tested extract to determine the content of the polyphenols in the to-be-tested sample. The method can be used to detect the content of polyphenols in the to-be-tested sample simply, quickly and accurately.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a liquid mass spectrometry detection method for polyphenols. Background technique [0002] Polyphenols are widely found in a variety of foods and medicines, for example, garlic contains a variety of polyphenols. In recent years, the analysis method for the content of polyphenolic compounds is mainly high-performance liquid chromatography, which has low sensitivity and poor accuracy. Moreover, the ingredients of garlic and other items are complex, and the pretreatment process of garlic and other samples by conventional methods is very cumbersome and time-consuming, which is not conducive to the rapid detection and quality control of garlic polyphenols, or uses a large amount of organic solvents, which is not environmentally friendly and harmful. economy. [0003] Therefore, the existing detection methods for polyphenols in food and medicine need to be improved. Contents of ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 张峰刘建王秀娟国伟杨敏莉
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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