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Raman rapid detection method for sibutramine in weight reducing tea

A detection method and technology for slimming tea, applied in Raman scattering, measurement devices, preparation of samples for testing, etc., can solve the problems of high experimental cost, difficulty in rapid detection and large-scale sampling activities, and achieve fast and sensitive detection methods. Recognition, the effect of broad application space

Pending Publication Date: 2020-04-03
SHANGHAI OCEANHOOD OPTO ELECTRONICS TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the high cost of experiments in the prior art, the detection time needs several hours, it is difficult to be used for rapid detection and large-scale sampling activities, and a Raman rapid detection method for sibutramine in slimming tea is proposed

Method used

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  • Raman rapid detection method for sibutramine in weight reducing tea
  • Raman rapid detection method for sibutramine in weight reducing tea

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Experimental program
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Embodiment 1

[0034] For the detection of different concentrations of sibutramine spiked in slimming tea, the specific steps are as follows:

[0035] 1. Standard product configuration: Accurately weigh 0.1g of sibutramine (accurate to 0.0001g), dissolve it in ultrapure water and set the volume to 10mL, marked as 10000ppm;

[0036] 2. Standard series solution configuration: Take 1mL, 2mL, and 5mL of the above-mentioned 10000ppm standard product and dilute to 10mL with ultrapure water, and mark them as 1000ppm, 2000ppm, and 5000ppm respectively.

[0037] 3. Sample addition: Take 1g of slimming tea solid particles into 15mL centrifuge tubes, add ultrapure water, 1000ppm, 2000ppm, 5000ppm sibutramine standard 100uL, mark as blank, 100ppm, 200ppm and 500ppm sibutramine Quming analog sample;

[0038] 4. Sample preparation: Add 10 mL of deionized water to the above centrifuge tube, vortex for 1 min;

[0039]5. Pretreatment: Take 1mL of the above slimming tea liquid sample in a 2mL centrifuge tub...

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Abstract

The invention discloses a Raman rapid detection method for sibutramine in weight-reducing tea. The method comprises the following operation steps of S1, preparing a sample, namely taking a proper amount of weight-reducing tea solid, adding an aqueous solution of which the mass volume is 5-50 times of the mass volume of the weight-reducing tea solid, and carrying out vortex oscillation for 1 min orultrasonic extraction for about 3 min to obtain a weight-reducing tea liquid; S2, pre-treating, namely centrifuging the weight-reducing tea liquid obtained in the step S1 until the weight-reducing tea liquid is clarified, taking 1 volume of supernatant, adding 0.5-2 volumes of ethyl acetate or similar organic solvents, carrying out oscillation extraction, and carrying out standing layering; taking out an organic phase, adding GCB according to a ratio of 0.01-0.05 g / mL, oscillating, taking out the organic phase, adding 0.5-2 volumes of 1mM-1M acid liquor, carrying out back extraction, and taking the water phase for detection; and S3, detecting, namely adding the water-phase sample to be detected in S2 into a Raman detection tube, adding metal nanoparticles and a coagulant, and uniformly mixing to carry out Raman detection. According to the method, the sibutramine in the weight-reducing tea can be quickly and sensitively identified, the pretreatment is simple, a reagent is cheap, the detection mode is quick, and the method has wide application space in the field of quick food detection.

Description

technical field [0001] The invention relates to the technical field of detection of prohibited weight-loss drugs in health products, in particular to a rapid Raman detection method for sibutramine in slimming tea. Background technique [0002] Sibutramine and its hydrochloride are central nervous system depressants, which have excitatory, antidepressant, and food-inhibiting effects. It acts mainly through its metabolites of amines (secondary and primary amines), and its main mechanism is to inhibit the reuptake of norepinephrine, serotonin and dopamine to enhance satiety. In 1997, sibutramine was approved by the US FDA for marketing. Because sibutramine may increase the risk of heart disease and stroke for users, the China Food and Drug Administration has forcibly stopped production and sales in mainland China in October 2010. [0003] There is no current national or industry standard for the detection of sibutramine in health food, but China Food and Drug Administration i...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/28
CPCG01N1/28G01N21/658
Inventor 陈仕良赵智慧陈娟
Owner SHANGHAI OCEANHOOD OPTO ELECTRONICS TECH
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