Raman rapid detection method for sibutramine in weight reducing tea
A detection method and technology for slimming tea, applied in Raman scattering, measurement devices, preparation of samples for testing, etc., can solve the problems of high experimental cost, difficulty in rapid detection and large-scale sampling activities, and achieve fast and sensitive detection methods. Recognition, the effect of broad application space
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[0034] For the detection of different concentrations of sibutramine spiked in slimming tea, the specific steps are as follows:
[0035] 1. Standard product configuration: Accurately weigh 0.1g of sibutramine (accurate to 0.0001g), dissolve it in ultrapure water and set the volume to 10mL, marked as 10000ppm;
[0036] 2. Standard series solution configuration: Take 1mL, 2mL, and 5mL of the above-mentioned 10000ppm standard product and dilute to 10mL with ultrapure water, and mark them as 1000ppm, 2000ppm, and 5000ppm respectively.
[0037] 3. Sample addition: Take 1g of slimming tea solid particles into 15mL centrifuge tubes, add ultrapure water, 1000ppm, 2000ppm, 5000ppm sibutramine standard 100uL, mark as blank, 100ppm, 200ppm and 500ppm sibutramine Quming analog sample;
[0038] 4. Sample preparation: Add 10 mL of deionized water to the above centrifuge tube, vortex for 1 min;
[0039]5. Pretreatment: Take 1mL of the above slimming tea liquid sample in a 2mL centrifuge tub...
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