Furan flavonoid compound in nicotiana tobacum and application thereof

A technology of furan flavonoids and compounds, applied in the field of tobacco chemical research, can solve the problems of unsatisfactory control effects, etc., and achieve the effect of good anti-tobacco mosaic virus, good effect and low cost

Active Publication Date: 2012-07-18
YUNNAN RES INST OF TOBACCO SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the control effect of the plant virus control agent developed at present is not ideal, and it is far from satisfying the needs of agriculture.

Method used

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  • Furan flavonoid compound in nicotiana tobacum and application thereof
  • Furan flavonoid compound in nicotiana tobacum and application thereof
  • Furan flavonoid compound in nicotiana tobacum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1——Isolation and preparation of compounds.

[0030] 1. 3.2 kilograms of air-dried tobacco stems and roots are pulverized, extracted and filtered at room temperature with 95% (3 × 3.0L) aqueous methanol solution, and the filtrate is evaporated and concentrated under reduced pressure to obtain the crude extract (196.5g ), and then the crude extract was subjected to silica gel chromatography with chloroform / methanol as eluent gradient elution (20:1, 9:1, 8:2, 7:3, 6:4, 5:5).

[0031] 2. Collect the 9:1 chloroform / methanol eluate and concentrate under reduced pressure. The concentrated solution was subjected to silica gel chromatography again, and chloroform / acetone was used as the eluent gradient elution (20:1, 8:2, 6:4, 5:5).

[0032] 3. Collect the 8:2 chloroform / acetone eluate and concentrate under reduced pressure. The concentrated solution was then separated by preparative high performance liquid chromatography, using methanol / water as the eluent for isocrat...

Embodiment 2

[0033] Example 2—the growth inhibitory rate test of the compound on tobacco mosaic virus.

[0034] 1. Drug for test: 20 μg·ml prepared by dissolving the compound of the present invention in DMSO -1 Concentration of the test solution, in this solution, the final concentration of DMSO is not more than 3%, with the same concentration of DMSO dissolved in aqueous solution as a control. In addition, dissolve Ningnanmycin in DMSO to prepare 20 μg·ml -1 concentration of the test solution was used as a positive control.

[0035] 2. Test method: The half-leaf method was used to test the growth inhibition rate of tobacco mosaic virus.

[0036] 3. Experimental result: experiment shows, active compound of the present invention is to the growth inhibitory rate experiment of tobacco mosaic virus, and compound of the present invention is 20 μ g·ml at concentration. -1 The growth inhibition rate of tobacco mosaic virus is 38.4%, and the growth inhibition rate of Ningnanmycin as a positive ...

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Abstract

The invention belongs to the field of the chemical study of nicotiana tobacum, and particularly relates to a furan flavonoid compound separated from the nicotiana tobacum and application of the furan flavonoid compound to prevention and control of tobacco mosaic virus. According to the furan flavonoid compound, stems and roots of the nicotiana tobacum, which serve as raw materials are extracted and separated to obtain a compound 3,4'-dimethoxy-5-hydroxyl-7,8-(2''-ethyl furan)-flavone, wherein a structural formula of the compound is shown in the specification. The furan flavonoid compound has the advantages of environment friendliness, low cost, good effect of resisting the tobacco mosaic virus and the like, does not harm human and livestock and can be used as a novel plant virus prevention and control agent for tobacco mosaic virus.

Description

technical field [0001] The invention belongs to the field of tobacco chemistry research, more specifically, the invention relates to a novel furan flavonoid compound isolated from tobacco and its application in preventing and treating tobacco mosaic virus. Background technique [0002] Tobacco mosaic virus, abbreviated as TMV, is the pathogen of tobacco mosaic disease and belongs to the Tobamovirus group. After tobacco plants are infected, the lateral veins and branch veins of young leaves are translucent, that is, bright veins. The mesophyll tissue on both sides of the veins gradually turns light green. The virus multiplies in a large amount in the leaf tissue, causing some mesophyll cells to increase or increase, resulting in uneven thickness of the leaves, yellow and green in color, and a mosaic shape. The mottled degree of the back mosaic leaves increases, and large areas of dark brown necrotic spots appear, especially in the middle and lower parts of the old leaves. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A01P1/00
Inventor 谭建林陈永宽苏钟璧王璐杨光宇张承明缪明明
Owner YUNNAN RES INST OF TOBACCO SCI
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