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Method for identifying two flue-cured tobaccos with different odor types in Guizhou

A technology of flue-cured tobacco and flavor type, applied in the field of tobacco chemistry, can solve the problems such as difficult to give objective, quantitative and digital descriptions, and achieve the effects of objective discrimination results, simple data processing, and simple sample processing methods

Inactive Publication Date: 2014-12-31
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] At present, the traditional discrimination of tobacco flavor type and aroma style is carried out by sampling and manual evaluation. It is carried out through aroma profile analysis, and it is difficult to give an objective, quantitative and digital description.

Method used

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  • Method for identifying two flue-cured tobaccos with different odor types in Guizhou
  • Method for identifying two flue-cured tobaccos with different odor types in Guizhou
  • Method for identifying two flue-cured tobaccos with different odor types in Guizhou

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Differentiation of two types of flue-cured tobacco with different aroma in Guizhou by fluorescence intensity scores in fluorescence spectroscopy

[0034] Collect 7 kinds of tobacco areas in Guizhou to grade C 3 F (medium orange three) flue-cured tobacco leaves 125 samples, each with a sampling amount of 1 kg, dried in an oven at 40°C for 6 hours, crushed, and passed through a 40-mesh sieve. Accurately weigh 1g of smoke sample, infiltrate with 30ml of chromatographic pure petroleum ether, shake for 2 hours and then stand for 24 hours. Take 0.5 mL of the supernatant after the above sample treatment method, dilute it with chromatographic pure petroleum ether 20 times, and transfer it to a polished non-fluorescent quartz cuvette with a thickness of 1 cm on all sides. The excitation wavelength is 200~500 nm and the emission wavelength is 200 ~600 nm range, with 2.0 nm as the wavelength increment, the exit and entrance slits are both 5.0 nm, and the detector multiplie...

Embodiment 2

[0036] Example 2: Identification of two types of different flavor flue-cured tobacco in Guizhou by the ratio of fluorescence intensity in fluorescence spectroscopy

[0037] The method of sample collection, processing and spectrometry is the same as that of the first embodiment. Calculate the fluorescence intensity ratio R of the 3 characteristic fluorescence peaks 1 , R 2 , R 3 . Where R 1 =FⅠ / FⅡ, R 2 = FⅡ / FⅢ, R 3 = FⅢ / FⅠ. Obtain the three-dimensional fluorescence intensity ratio data of each smoke sample (Table 2). Use SPSS17.0 data statistical analysis software to define “sample number, R 1 , R 2 , R 3 "4 variables, of which "sample number" is a string type, and the other three are numeric types. Enter the sample number of different tobacco planting areas and the fluorescence intensity ratio data of the tobacco leaf petroleum ether extract in each area into the "data view" and select "Analysis"→"Classification"→"Systematic Cluster Analysis" operation function. Among them, "...

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Abstract

The invention relates to a method for identifying two flue-cured tobaccos with different odor types in Guizhou, belonging to the field of tobacco chemistry. The method disclosed by the invention adopts a three-dimensional fluorescence spectrum technology to perform clustering identification on the two different flue-cured tobaccos, namely fresh, sweet and aromas flue-cured tobaccos planted in the southwest of Guizhou province, Liupanshui and Bijieyu, and mellow, sweet and aromas flue-cured tobaccos planted in the southeast of Guizhou province, Tongren, Zunyi and the south of Guizhou province. Relative content difference information of chemical substances in the flue-cured tobaccos of different aromas types is obtained by measuring overall characteristic spectrums of chemical components in petroleum ether extracting solutions of tobacco leaves according to positions and intensities of characteristic spectrum peaks; and clustering analysis is carried out by statistical analysis software. The characteristic spectrum detection method is capable of identifying the aromas types of the two flue-cured tobaccos in Guizhou rapidly, simply, conveniently and accurately.

Description

technical field [0001] The invention belongs to the field of tobacco chemistry, and specifically relates to a method for distinguishing two types of flue-cured tobaccos with different flavors in Guizhou, that is, a method for distinguishing two types of flue-cured tobaccos with different flavors: "clear and sweet" and "mellow and sweet". Background technique [0002] Tobacco aroma is the result of the joint action of various aroma components, and it is an important indicator for evaluating the quality of tobacco and its products. The aroma quality and flavor type of tobacco leaves are directly related to the types and contents of aroma substances. There are many kinds of substances that form tobacco aroma, and the content of the ingredients is very small, and there are extremely complex interactions among various aroma components. The aroma type and aroma quality of tobacco leaves in different planting areas are related to type, variety, ecological environment and cultivati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 冉霞牟兰刘仁祥曾晞
Owner GUIZHOU UNIV
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