Method for determining content of pigments in mixed pigment solutions by adopting three-dimensional fluorescence spectroscopy combined with PARAFAC (parallel factor analysis) algorithm

Abstract

The invention discloses a method for determining the content of pigments in mixed pigment solutions by adopting three-dimensional fluorescence spectroscopy combined with the PARAFAC (parallel factor analysis) algorithm. According to the method, firstly, a correction sample set is formed by preparing multiple mixed pigment solutions according to different concentrations, then three-dimensional fluorescence spectra of the correction sample set and the background are measured, measured signals are pre-processed, a three-dimensional data matrix after pre-processing is analyzed with the PARAFAC algorithm, a correction model is established according to an analysis result, and the content of the pigments in unknown samples is predicted with the correction model. The method overcomes the defects of tedious process, time consumption of detection, higher detection cost and the like of a traditional chemical detection method, the concentration of each pigment in the to-be-detected samples can be determined rapidly, the method has the advantages of rapidness, safety, reliability, simple operation, lower cost and the like, technical support is provided for determination of synthetic edible pigments in food, and supervision of the food industry and protection of consumer interests are facilitated.

Description

Technical field [0001] The invention provides a method for measuring the pigment content in a mixed pigment solution by combining three-dimensional fluorescence spectroscopy with a PARAFAC algorithm, and belongs to the field of quantitative detection of food pigments. Background technique [0002] Food safety issues are related to people's health and lives, and have always been valued by the state. However, the current situation of food safety is not optimistic. New food safety issues continue to emerge, and the illegal use of synthetic food colorants accounts for a considerable proportion. Food pigments are a kind of food additives whose main purpose is to adjust the color of food, also known as colorants, which have been widely used in the food industry and the production of medicine and cosmetics. There are two types of food pigments: synthetic pigments and natural pigments. Among them, synthetic pigments are widely used because of their bright color, strong coloring power, a...

AI Technical Summary

Problems solved by technology

However, many synthetic food pigments have certain toxicity, such as carmine, allura red, amaranth and other pigments, which may cause teratogenicity and cancer if excessive intake of pigments, so the variety, scope and quantity of use must be strictly controlled

At present, the methods for detecting food pigment content mainly include: reversed-phase high-performance liquid chromatography (RP-HPLC) method, chemiluminescence method, RP-HPLC fluorescence detection method, etc., the above-mentioned methods generally have complex operations, time-consuming detection, high detection cost, The results have disadvantages such as poor reproducibility. Therefore, it is very necessary to find a fast, convenient, stable and reliable food coloring content measurement technology.

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