Three-dimensional fluorescent differential super-resolution microscopic method and device

Abstract

The invention discloses a three-dimensional fluorescent differential super-resolution microscopic device which comprises a laser, an electric sample stage carrying a sample to be detected, and a microobjective for projecting light rays to the electric sample stage, wherein a polarizer for converting a beam emitted by the laser into linear polarized light, a first half wave plate for modulating a polarization direction of the linear polarized light, a space light modulation module for sequentially modulating a horizontal component and a vertical component of the beam and a scanning galvanometer system for light path deflection of circularly polarized light are sequentially arranged between the laser and the microobjective; the circularly polarized light emitted by the scanning galvanometer system is projected by the microobjective onto the sample to be detected; the device further comprises a detection system acquiring signal light emitted by the sample to be detected, and a computer controlling the space light modulation module and the scanning galvanometer system. The invention further discloses a microscopic method achieved on a basis of the three-dimensional fluorescent differential super-resolution microscopic device.

Description

technical field [0001] The invention belongs to the field of optical super-resolution microscopy, in particular to a three-dimensional fluorescent differential super-resolution microscopy method and device. Background technique [0002] In 1873, the German scientist Abbe proposed the "diffraction limit" of the optical imaging system. Any optical microscope has a resolution limit, which is determined by the wavelength of light and the numerical aperture of the lens. Due to the existence of the "diffraction limit", optical microscopy imaging systems cannot achieve high-resolution imaging below 200 nanometers in the visible light band. For this reason, people continue to work hard to research and develop super-resolution imaging technology, hoping to break through the diffraction limit and obtain higher resolution. In 2014, three scientists engaged in fluorescence super-resolution optical microscopy won the Nobel Prize in Chemistry. They opened the door for humans to use fluor...

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Problems solved by technology

Due to the existence of the "diffraction limit", the optical microscopy imaging system

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