The application discloses a method for programmable fast
assembly of SERS substrates driven by photo-induced
dielectrophoresis, and relates to the technical fields of micro-
nano manufacturing and surface enhanced Raman spectrum detection, and the method comprises the following steps: constructing a photo-induced
electrophoresis chip; obtaining micro-nano particles and target objects to be detected; mixing the micro-nano particles and the target objects to be detected to obtain a suspension, and injecting the suspension into a liquid cavity of the photo-induced
electrophoresis chip to obtain an assembled photo-induced
electrophoresis chip; based on the assembled photo-induced electrophoresis chip, driving the micro-nano particles in the suspension to capture the target objects to be detected according to photo-induced
convection and
dielectrophoresis force through a programmable
spatial light modulation device, obtaining a chimeric substrate, and performing in-situ surface enhanced Raman spectrum detection and quantitative analysis. The application solves the problems of low efficiency, poor structure
controllability, insufficient uniformity and
liquid phase incompatibility of traditional micro-nano
assembly technology, and realizes efficient aggregation, reconfigurable
assembly and in-situ SERS detection of the micro-nano particles and the target objects to be detected in a liquid environment.