Discrete three-dimensional fluorescence spectrum-based phytoplankton identification and measurement method and discrete three-dimensional fluorescence spectrum-based phytoplankton identification and measurement device
A phytoplankton, three-dimensional fluorescence technology, applied in the field of environmental science, can solve the problems of high operation stability, large volume and high power consumption, and achieve the effects of high identification and measurement ability, simple and compact structure, and easy portability.
Active Publication Date: 2014-06-18
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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However, due to the large size, high power consumption, and high requirements for operational stability of traditional three-dimensional fluorescence spectroscopy measuring instruments, the phytoplankton identification and determination method based on three-dimensional fluorescence spectroscopy remains at the stage of laboratory sample analysis, and it is difficult to meet the requirements of phytoplankton on-site. Identify assay needs
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[0045] Select Microcystis aeruginosa from Cyanophyta, Chlorella vulgaris from Chlorophyta, Fragilaria sp from Diatoms, Glenodinium gymnodinium from Dinophyta and Cryptophyta For the experimental algae species of Cryptomonas ovata, the 15-day pure culture of the above five algae was used to measure the chlorophyll a concentration of each pure culture by high performance liquid chromatography. Dilute a certain number of times as the sample mother solution, calculate the chlorophyll a concentration of the sample mother solution according to the dilution ratio, and control the chlorophyll a concentration of the mother solution within the range of 80-100 μg / L. Then, the five kinds of algae mother liquors were half-diluted with distilled water into three pure samples of concentration gradients, and then mixed according to the volume ratio shown in Table 2 to prepare 26 kinds of planktonic algae mixed samples.
[0046] Table 1 prepares the volume ratio of the mixed sample of plankton...
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Abstract
The invention discloses a discrete three-dimensional fluorescence spectrum-based phytoplankton identification and measurement method and a discrete three-dimensional fluorescence spectrum-based phytoplankton identification and measurement device. The measurement method comprises the following steps: selecting a characteristic fluorescence peak which corresponds to different photosynthetic pigments as an analytic target according to fingerprint characteristics of a phytoplankton fluorescence spectrum, maintaining the phytoplankton characteristic information in the three-dimensional fluorescence spectrum to the greatest degree, fitting a characteristic spectrum of the sample according to each characteristic spectrum in a standard discrete three-dimensional fluorescence spectrum library, and inverting the types and the content of the phytoplankton in the sample. According to the measurement device, a multiband light-emitting diode (LED) array serves as an excitation light source, and a multiband light filter group serves as a band selector for receiving fluorescence. The device is simple and compact in structure, small in size and convenient to carry and is suitable for identification and measurement of field phytoplankton, the phytoplankton identification and measurement method provided by the invention is combined, so that rapid classified measurement on the phytoplankton site can be realized.
Description
technical field [0001] The invention relates to the field of environmental science, in particular to a method and device for identifying and measuring phytoplankton based on discrete three-dimensional fluorescence spectrum. Background technique [0002] The species and quantity of phytoplankton can reflect the ecological status of the area. Phytoplankton has long been used as an indicator of water quality. Especially for large areas of water, the determination of the species and quantity of phytoplankton has more important practical significance for water quality evaluation. . With the eutrophication of my country's inland lakes and offshore waters, the excessive proliferation of phytoplankton in the water body, frequent occurrence of red tides and algae blooms, there is an urgent need to develop technologies and equipment that can directly and quickly analyze phytoplankton species and data, and provide more information for red tides and water blooms. China's monitoring and ...
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IPC IPC(8): G01N21/64
Inventor 殷高方赵南京胡丽石朝毅余晓娅方丽段静波肖雪甘婷婷董欣欣张玉钧刘建国刘文清
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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