Method and device for identifying and measuring phytoplankton based on discrete three-dimensional fluorescence spectroscopy

Abstract

The invention discloses a discrete three-dimensional fluorescence spectrum-based phytoplankton identification and measurement method and a discrete three-dimensional fluorescence spectrum-based phytoplankton identification and measurement device. The measurement method comprises the following steps: selecting a characteristic fluorescence peak which corresponds to different photosynthetic pigments as an analytic target according to fingerprint characteristics of a phytoplankton fluorescence spectrum, maintaining the phytoplankton characteristic information in the three-dimensional fluorescence spectrum to the greatest degree, fitting a characteristic spectrum of the sample according to each characteristic spectrum in a standard discrete three-dimensional fluorescence spectrum library, and inverting the types and the content of the phytoplankton in the sample. According to the measurement device, a multiband light-emitting diode (LED) array serves as an excitation light source, and a multiband light filter group serves as a band selector for receiving fluorescence. The device is simple and compact in structure, small in size and convenient to carry and is suitable for identification and measurement of field phytoplankton, the phytoplankton identification and measurement method provided by the invention is combined, so that rapid classified measurement on the phytoplankton site can be realized.

Description

Technical field [0001] The invention relates to the field of environmental science, in particular to a method and device for identifying and measuring phytoplankton based on discrete three-dimensional fluorescence spectroscopy. Background technique [0002] The species and quantity of phytoplankton can reflect the ecological status of the area. Phytoplankton has been used as an indicator of water quality for a long time. Especially for large areas of water, the determination of the species and quantity of phytoplankton has more important practical significance for water quality evaluation. . With the eutrophication of inland lakes and offshore waters in my country, resulting in excessive proliferation of phytoplankton and frequent occurrence of red tides and water blooms, there is an urgent need to develop direct and rapid phytoplankton species and data analysis technologies and equipment for red tides and water China monitoring and early warning provide important means. [0003] ...

AI Technical Summary

Problems solved by technology

However, due to the large size, high power consumption, and high requirements for operational stability of traditional three-dimensional fluorescence spectroscopy measuring instruments, the phytoplankton identification and determination method based on three-dimensional fluorescence spectroscopy remains at the stage of laboratory sample analysis, and it is difficult to meet the requirements of phytoplankton on-site. Identify assay needs

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