Method for simultaneously and quickly detecting benzo[a]pyrene (BaP) and benzo[k]fluoranthene (BKP)

Abstract

A method for simultaneously and quickly detecting benzo[a]pyrene (BaP) and benzo[k]fluoranthene (BKP) is characterized in that normal hexane is used as a solvent to prepare 16 kinds of 0.025ppm PAHs standard samples; a fluorescence spectrophotometer is used for scanning under the condition that the delta lambada is equal to 137nm, which is obtained from experimental optimization; only the fluorescence spectrograms with higher resolution of the BaP and BKF appear within the range of 230-350nm of emission wavelength; the synchronous fluorescence peak corresponds to the BaP in the position of 265.79nm while to the BKF in the position of 291.85nm. As the fluorescence spectrograms of the BaP and BKF overlap, the Lambert-Beer law can be adopted to derive the quantitative relation between the fluorescence intensity and the concentration of the two substances to obtain the equations set as above. Qualitative and quantitative detection is carried out on the BaP and BKF according to the fluorescence spectrograms of the BaP and BKF, which are obtained through scanning of the fluorescence spectrophotometer under the condition that the delta lambada is equal to 137nm and the equations set.

Description

technical field [0001] The invention belongs to a fluorescence spectrum measurement method, in particular to a detection method for polycyclic aromatic hydrocarbons. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are typical persistent organic pollutants, which are toxic, bioaccumulative, semi-volatile, and widely distributed in the environment. Among them, benzo[a]pyrene (abbreviated as BaP, the same below), which is the most carcinogenic and the most harmful to humans, has been used as an indicator of PAHs pollution in the environment, so it is particularly important for the detection of BaP [1-3] . [0003] At present, there are many detection methods for PAHs, such as gas chromatography (GC), liquid chromatography (LC), fluorescence method, etc. [4-8] . Among them, GC needs to consume carrier gas, and it is difficult to separate substances with high boiling point and poor thermal stability. LC can well make up for the shortcomings of GC, but th...

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Some studies have shown that the selection range of Δλ is wide

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