Application of protein in predicting drug performance

Abstract

The invention discloses application of a protein in predicting the drug performance, and belongs to the field of protein application. The protein is used for predicting the drug performance, and drugs include pesticides, human drugs and veterinary drugs. The application is implemented by the steps of preparing a 0.02 M phosphate buffer solution with the pH value of 7.4, and dissolving and diluting a protein solution by using the prepared buffer solution according to a signal value; and mixing the prepared protein diluent with a drug to be determined according to a molar ratio of 1: (1-300), and predicting the performance of the drug by using a fluorescence spectrum, or synchronous fluorescence, or three-dimensional fluorescence, or circular dichroism spectrum, or UV-Vis absorption spectrum, or linear spectrum, or banded spectrum. A BP neural network model among five indexes (a quenching constant, a binding site number, a binding constant, free energy and a binding distance) is obtained through a fluorescence spectrum and toxicity so as to predict the drug performance, and the application has important practical significance on the development of the drug research and development industry.

Description

technical field [0001] The invention belongs to the field of protein applications, in particular to the application of a protein in predicting the performance of medicines. Background technique [0002] All new drugs must go through a series of tests before entering the market. Toxicity is an important indicator and the main reason for drug development failure, which has been reported to lead to the consumption of about 30% of drug candidates. In particular, many drugs have been withdrawn from the market due to high toxicity in the late stage of development, which is not only a waste of time, but also a waste of cost. Several years ago, it was suggested that toxicological evaluation should be brought forward in the early drug discovery process for small molecules. Early assessment of drug toxicity helps to achieve precise drug design and synthesis, effectively shortening the drug development cycle and reducing costs. Many strategies and new methods for early prediction of...

AI Technical Summary

Problems solved by technology

However, most pesticides are toxic and pose a threat to the environment, humans, animals and other living things

With the increasing awareness of human environmental protection, many pesticides are prohibited from using products with good activity but high toxicity

Especially carbamate pesticide is a kind of pesticide widely used in agricultural production, but once it enters the human body, it may cause the danger of acute or chronic poisoning

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