Anoectochilus formosanus tea drink as well as preparation method and production system thereof

Abstract

The invention belongs to the technical field of tea drinks, and particularly relates to an anoectochilus formosanus tea drink as well as a preparation method and a production system thereof. The anoectochilus formosanus tea drink is prepared from anoectochilus formosanus, cassia seeds, penthorum chinense pursh, abrus cantoniensis hance, chrysanthemum buds, liquorice, jasmine flowers, momordica grosvenori, chrysanthemum morifolium ramat and double-petal red roses. Through reasonable matching of the raw materials, the anoectochilus formosanus tea drink has a good taste and a health care effect, and the original poor taste of tea drinks taking anoectochilus formosanus as a main material is effectively improved. The anoectochilus formosanus tea drink is prepared into a small package or cup tea form, is convenient for consumers to brew and drink anytime and anywhere, meets the requirements of modern fast-paced life, and has a wide market prospect.

Description

Technical Field

[0001] The present invention belongs to the technical field of tea beverages, and in particular relates to a roxburghii tea beverage, a preparation method and a production system thereof. Background Art

[0002] As people's health awareness increases, the demand for beverages with health benefits is increasing. Traditional tea beverages have relatively simple functions. However, if some plants with medicinal value can be reasonably combined to make tea drinks, they can not only meet people's daily beverage needs, but also play a certain health-promoting role, and have broad market prospects.

[0003] Anoectochilus roxburghii has various medicinal benefits, such as clearing heat and cooling blood, dispelling wind and dampness, etc. However, drinking Anoectochilus roxburghii alone has a poor taste and limited efficacy. Currently, the market lacks a tea formula that is primarily based on Anoectochilus roxburghii and combined with other botanical ingredients, has a good taste and significant comprehensive health benefits. Summary of the Invention

[0004] Based on the above technical background, the main purpose of the present invention is to provide a golden thread lotus tea, a preparation method and a production system thereof, so as to overcome the shortcomings of the existing technology.

[0005] To achieve the aforementioned object of the invention, the technical solutions adopted by the present invention include: The first aspect of the present invention is to provide a roxburghii tea beverage, which is prepared from the following raw materials in parts by weight: 2-5 weight parts of roxburghii, 1-4 weight parts of cassia seed, 1-3 weight parts of scutellaria baicalensis, 1-3 weight parts of chrysanthemum, 1-4 weight parts of chrysanthemum rice, 0.5-2 weight parts of liquorice, 0.5-2 weight parts of jasmine, 0.5-2 weight parts of momordica grosvenori, 0.3-1 weight parts of chrysanthemum, and 0.5-1.5 weight parts of double red rose.

[0006] Preferably, the anoectochilus roxburghii tea is prepared from the following raw materials in parts by weight: 3-4 weight parts of roxburghii, 2-3 weight parts of cassia seed, 2 weight parts of huanghuangcao, 2 weight parts of jigucao, 1 weight part of chrysanthemum rice, 1 weight part of liquorice, 1 weight part of jasmine, 1 weight part of momordica grosvenori, 0.4-0.7 weight part of chrysanthemum, and 0.7-1.2 weight parts of double red rose.

[0007] More preferably, the anoectochilus roxburghii tea is prepared from the following raw materials in parts by weight: 4 parts by weight of roxburghii, 3 parts by weight of cassia seed, 2 parts by weight of huangcao, 2 parts by weight of jiguca, 3 parts by weight of chrysanthemum rice, 1 part by weight of licorice, 1 part by weight of jasmine, 1 part by weight of momordica grosvenori, 0.5 parts by weight of chrysanthemum, and 1 part by weight of double red rose.

[0008] Or, 3 parts by weight of roxburghii, 2 parts by weight of cassia seed, 2 parts by weight of huangcao, 2 parts by weight of jigucao, 1 part by weight of chrysanthemum rice, 1 part by weight of licorice, 1 part by weight of jasmine, 1 part by weight of momordica grosvenori, 0.6 parts by weight of chrysanthemum, and 0.9 parts by weight of double red rose.

[0009] The raw materials used in the present invention have the following effects: Golden thread lotus (Roxb. roxb.) is cool in nature and sweet in flavor. It enters the liver, lung, and kidney meridians, clearing heat, cooling blood, removing dampness, and detoxifying. It can treat coughing up phlegm and blood caused by tuberculosis and is often used in combination with lily and gardenia. Golden thread lotus (Roxb. roxb.) clears heat and cools blood, and can treat muscle spasms, redness, swelling, and pain caused by arthritis. It can also treat urinary pain and hematuria caused by urinary tract infections and can be used in combination with Imperata rhizome. The active ingredients in Golden thread lotus can lower transaminases and have a certain effect on mild transaminase elevations caused by fatty liver.

[0010] Cassia seed clears the liver and improves eyesight, moistens the intestines and promotes bowel movements. It's suitable for those suffering from red, swollen, and painful eyes, photophobia, excessive tearing, and blurred vision; headaches, dizziness, and constipation caused by dry intestines. For those suffering from red, swollen, and painful eyes, photophobia, excessive tearing, and blurred vision: Cassia seed primarily enters the liver meridian and has the effect of clearing the liver and improving eyesight. It can be used to treat red, swollen, and painful eyes, photophobia, and excessive tearing caused by liver heat, headaches and red eyes caused by wind-heat, or blurred vision and blurred vision caused by liver and kidney yin deficiency. Cassia seed has a bitter and cold nature and enters the liver, clearing liver fire and calming liver yang. It can be used to treat symptoms such as headaches and dizziness caused by hyperactivity of liver yang. Cassia seed is sweet, salty, and cold in nature, entering the large intestine meridian, and can clear heat, moisten the intestines, and promote bowel movements.

[0011] Herba Aspergiae (Ganhuangcao) is sweet and slightly cold in nature, primarily acting on the liver and kidney meridians. It has the effects of relieving jaundice and dampness, clearing away heat and toxins, promoting diuresis and removing dampness, relieving blood stasis and alleviating pain, and promoting blood circulation and removing stasis. It is used to treat jaundice, edema, dysuria, sores, and injuries from falls. Herba Aspergiae (Ganhuangcao) contains flavonoids, alkaloids, and polysaccharides. Modern research confirms that it has alcohol-relieving properties, anti-thrombotic effects, liver protection, and anti-fibrotic effects, as well as hepatitis B virus resistance.

[0012] Herba Cervi Paniculatae is sweet, slightly bitter and cool in nature and flavor. It enters the Liver and Stomach Meridians. It has the effects of promoting dampness and relieving jaundice, clearing away heat and toxic substances, soothing the liver and relieving pain, and is used to treat damp-heat jaundice, flank discomfort, epigastric distension and pain, and mastitis and pain. Herba Cervi Paniculae enters the Liver and Stomach Meridians and has the effects of soothing the liver and relieving pain. It can be used for flank discomfort and epigastric pain caused by liver qi stagnation. It can also clear away heat and toxic substances, and can also be used to treat mastitis and mastitis. It also has the effects of clearing away heat and promoting dampness, relieving jaundice, and can be used to treat jaundice caused by liver and gallbladder heat. Modern pharmacological studies have shown that Herba Cervi Paniculae has the effects of lowering blood lipids, protecting the liver, resisting liver fibrosis, antibacterial and antiviral, anti-inflammatory, and antioxidant.

[0013] Chrysanthemum rice contains a variety of nutrients, including protein, wild chrysanthemum flavonoids, chrysanthemum lactones, amino acids, and trace elements, which can supplement the nutrients needed by the human body. It has the effects of calming the liver and suppressing yang, dispersing wind-heat, clearing away heat and detoxifying, nourishing the kidneys, clearing away summer heat and relieving hangovers, protecting against radiation, and preventing colds. It also dispels wind, improves eyesight, clears heat, and relieves boils. It can lower blood lipids and blood pressure, and prevent cardiovascular and cerebrovascular diseases. Regular consumption can strengthen the body, improve disease resistance, and promote longevity.

[0014] Licorice has the functions of tonifying the spleen and replenishing qi, clearing away heat and detoxifying, removing phlegm and relieving cough, relieving pain, and harmonizing various medicines. It can be used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, cough with sputum, cramps and pain in the limbs, carbuncles and sores, and to relieve the potency and toxicity of drugs.

[0015] Jasmine is warm in nature and sweet in taste. It has the effects of promoting blood circulation, regulating qi and relieving pain, dispelling filth and relieving depression, warming the middle and harmonizing the stomach, and detoxifying. It is beneficial for some abdominal pain, diarrhea, and mouth sores. It can also effectively relieve eye fatigue, promote fluid production and quench thirst, and promote urination. It has certain effects on symptoms such as edema and colds.

[0016] Luo Han Guo (Sheng Han Guo) is sweet and cooling in nature, and enters the Lung and Large Intestine meridians. It has been shown to clear heat and moisten the lungs, relieve sore throat and improve voice, promote bowel movements, lower blood sugar, protect the liver, provide anti-aging and anti-cancer benefits, enhance beauty, regulate blood sugar, aid in weight loss, and boost immunity. It is clinically used to treat lung heat-induced dry cough, sore throat and loss of voice, and constipation caused by dry intestines.

[0017] 1.1 Chrysanthemum has the benefits of dispersing wind-heat, calming liver yang, clearing the liver and improving eyesight, clearing away heat and detoxifying, and relieving asthma and cough. Chrysanthemum is rich in amino acids, chrysanthemum glycosides, and other essential trace elements for the human body. Traditional Chinese medicine research shows that chrysanthemum can nourish the kidneys, strengthen the stomach, promote fluid production and moisten the throat, and clear the heart. Chrysanthemum tea can inhibit Escherichia coli, Shigella dysenteriae, Staphylococcus aureus, Salmonella typhi, Vibrio cholerae, and Mycobacterium tuberculosis.

[0018] Double-petaled red roses are sweet, slightly bitter, and warm. Roses travel to the liver and spleen meridians, promoting qi circulation, relieving depression, and promoting blood circulation to relieve pain. Roses are known for their ability to regulate liver qi and are used to treat stomach aches caused by liver qi stagnation, as well as nausea and vomiting. They are also used to treat irregular menstruation, dysmenorrhea, and injuries from falls, relieving pain and promoting blood circulation.

[0019] The second aspect of the present invention is to provide a method for preparing the roxburghii tea according to the first aspect of the present invention, the preparation method comprising the following steps: The method comprises the following steps: weighing roxburghii, cassia seed, chrysanthemum herb, chrysanthemum root, liquorice, jasmine flower, momordica grosvenori, chrysanthemum and double-petal red rose, washing, drying, extracting fragrance, crushing and packaging to obtain roxburghii tea.

[0020] Preferably, the drying conditions are: drying at 35-45° C. for 3-5 hours.

[0021] More preferably, the drying condition is: drying at 40° C. for 4 h.

[0022] The drying temperature should not be too high, as too high a temperature will destroy the effective ingredients in the raw materials. However, too low a temperature will not be conducive to the removal of moisture in the raw materials and will shorten the shelf life of the golden thread vine tea.

[0023] Preferably, the roxburghii roxburghii, cassia seed, chrysanthemum herb, chrysanthemum root, liquorice, jasmine, momordica grosvenori, chrysanthemum and double-petal red rose are crushed into 10-30 meshes.

[0024] More preferably, the angelica dahurica, cassia seed, chrysanthemum herb, chrysanthemum root, liquorice, jasmine, momordica grosvenori, chrysanthemum and double-petal red rose are crushed into 10-20 meshes.

[0025] After drying, the powder is crushed to a particle size of 10-20 mesh to facilitate the subsequent extraction of active ingredients. Licorice, jasmine, and monk fruit are crushed appropriately to increase their surface area, which is beneficial for the dissolution of active ingredients in the tea during soaking.

[0026] The mixed raw materials are packaged separately, and filter paper bags and other packaging materials can be used to make small packages (such as 5 grams per bag) for consumers to brew and drink conveniently.

[0027] Alternatively, the mixed raw materials are packaged and used for preparing cup tea.

[0028] The third aspect of the present invention is to provide a production system for preparing the golden thread vine tea described in the first aspect of the present invention, wherein the production system includes a cleaning device, a drying device, a crushing device, a hopper, a feeding device, and a pressing device; the cleaning device, the drying device, the crushing device, the hopper, the feeding device, and the pressing device are connected in sequence.

[0029] The cleaning device is used to clean the medicinal materials.

[0030] The drying device is used for drying the cleaned medicinal materials.

[0031] The pulverizing device is used for pulverizing the washed and dried medicinal materials.

[0032] The hoppers preferably include 18 to 25 hoppers, and more preferably include 20 hoppers.

[0033] The present invention sets up multiple hoppers, and the multiple hoppers are independently controlled. Various Chinese medicinal materials can be put into corresponding hoppers respectively. Each hopper is independently controlled, which can avoid mixed feeding and achieve the accuracy of single feeding, avoiding the problems of specific gravity, efficacy and taste of various formula materials caused by the "multi-material mixing and stirring feeding" of traditional equipment.

[0034] Before production, each hopper of the equipment must be thoroughly cleaned with medicinal alcohol to ensure that there are no residual impurities, laying the foundation for accurate packaging.

[0035] Each hopper provided in the present invention is provided with its own independent material discharge control system. The multiple hoppers provided in the present invention can operate independently without interfering with each other. The materials can be discharged simultaneously according to the preset dosage parameters of various formula materials, avoiding the error of "heavy materials falling first and light materials falling later". Traditional equipment is prone to imbalance in taste and efficacy due to mixed discharge. The hopper and discharge method described in the present invention can ensure the formula requirements of each cup of tea with higher uniformity.

[0036] After starting the equipment, first turn on the feeding system and set the packaging weight of each medicinal material through the touch screen (accuracy ±0.1g) to ensure that the medicinal material ratio for each cup of tea is accurate and controllable.

[0037] After starting the equipment, the preheating module is started simultaneously to provide an adaptive and stable temperature for the subsequent mesh cutting, ultrasonic filter bonding, and pressing processes, ensuring the effective production of packaging, mesh cutting, and bonding.

[0038] The packaged raw materials are added to the corn fiber filter, and the pressing device is used to press the packaged corn fiber filter. The pressing device uses ultrasonic technology to cut and bond the corn fiber filter, which can achieve efficient and firm packaging and improve food-grade products beyond industry standards.

[0039] The production system of the golden thread lotus tea beverage of the present invention has a high output: the daily output can reach 15,000 cups or more, which is about 150% higher than similar equipment on the market (an average of 6,000 cups per day), and can meet the needs of large-scale production.

[0040] The production system can achieve a packaging accuracy of ±0.1g for each type of medicinal material, far exceeding traditional equipment (which has poor mixing and feeding accuracy), and can ensure the stability of product dosage, taste and efficacy from the source.

[0041] The production system can achieve fully automated operation and reduce manual intervention. The 20-hopper design supports the simultaneous loading and packaging of multiple medicinal materials. If the formula has fewer than 10 ingredients, dual guide rails can be used for simultaneous packaging (traditional equipment has a maximum of 8 hoppers). Labor costs are reduced by about 50%, production efficiency is significantly improved, and output is doubled.

[0042] The beneficial effects of the present invention are: (1) The rosmarinus officinalis tea of ​​the present invention is prepared from rosmarinus officinalis, cassia seed, scutellaria baicalensis, chrysanthemum rice, liquorice, jasmine, momordica grosvenori, chrysanthemum and double red rose. By adding jasmine and momordica grosvenori, the unique aroma and sweetness of the jasmine and momordica grosvenori are utilized to effectively improve the originally poor taste of the rosmarinus officinalis tea, so that the brewed tea has a refreshing and sweet taste and is more easily accepted.

[0043] The present invention uses a reasonable combination of raw materials to cooperate with each other in health care effects, which can play a role in comprehensively regulating body functions, such as clearing away heat, detoxifying, moistening the lungs, and soothing the liver. The golden thread lotus tea of ​​the present invention has significant effects in preventing chronic inflammation and anti-inflammation.

[0044] The roxburghii tea has both good taste and health benefits. The present invention provides the roxburghii tea in small packages, making it convenient for consumers to brew and drink anytime, anywhere, meeting the needs of modern fast-paced lifestyles. The roxburghii tea can also be prepared as a cup of tea, also meeting the needs of consumers' fast-paced lifestyles.

[0045] (2) The independent control of multiple hoppers in the production system of the present invention breaks through the upper limit of "8 hoppers" of existing market equipment, supports separate unloading of multiple medicinal materials and simultaneous production on dual guide rails, adapts to diversified formula requirements, is particularly suitable for the precise packaging of Chinese medicinal compound tea, and can improve production capacity.

[0046] The production system of the present invention can prevent mixed feeding, avoid the disadvantage of "light and heavy material separation" of traditional equipment, ensure that the proportion of medicinal materials in each cup of tea strictly complies with the recipe design, and solve the problem of quality fluctuation caused by uneven feeding.

[0047] (3) The production system achieves high precision, high efficiency and high stability in the packaging of Chinese herbal medicine cups and teas, providing a technological innovation solution for the industrialized production of Chinese herbal medicine beverages. It is significantly superior to similar products on the market and helps companies improve product quality and market competitiveness. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 Schematic diagram showing the expression levels of inflammatory factors mRNA, NO content and pro-inflammatory factor IL-6 content in RAW264.7. Figure 1Middle: NC represents the negative control group; LPS represents the inflammation model group; ATW represents the aqueous extract of Anoectochilus roxburghii tea; ATC represents the ethanol extract of Anoectochilus roxburghii tea; HGT represents the aqueous extract of Polygonatum sibiricum and Panax ginseng tea; HGC represents the ethanol extract of Polygonatum sibiricum and Panax ginseng tea; AT represents the aqueous extract of Anoectochilus roxburghii; AT represents the ethanol extract of Anoectochilus roxburghii. (Values ​​are mean ± standard deviation. # indicates comparison with NC, ### < 0.0001, ##p < 0.001; * indicates comparison with LPS, ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05; $ indicates comparison between ATW and ATC, HGT and HGC, or AW and AC, SSSp < 0.0001, SSp < 0.01, ns, no significant difference).

[0049] Figure 2 Figure 2 shows the mRNA expression levels of inflammatory factors in RAW264.7 cells. NC represents the negative control group; LPS represents the inflammatory model group; PATW represents the aqueous extract of Anoectochilus roxburghii tea; PATC represents the ethanol extract of Anoectochilus roxburghii tea; PHGT represents the aqueous extract of Polygonatum sibiricum and Panax ginseng tea; and PHGC represents the ethanol extract of Polygonatum sibiricum and Panax ginseng tea. (Values ​​are mean ± SD. # indicates #p < 0.0001, #p < 0.01 compared with NC; * indicates ***p < 0.001, **p < 0.01, *p < 0.05 compared with LPS; $ indicates SSSp < 0.0001, SSp < 0.01 compared with PATW or PHGT vs. PHGC, ns, no significant difference.) Figure 3 A photo of a roxburghii tea bag prepared in Example 1 is shown; Figure 4 A photograph showing a cup of roxburghii tea prepared in Example 1 is shown. DETAILED DESCRIPTION

[0050] The present invention will be described in detail below, and the features and advantages of the present invention will become clearer and more distinct with the description. The raw materials used in the examples of the present invention were all purchased.

[0051] Example The present invention is further described below through specific examples. These examples are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.

[0052] Example 1 A roxburghii tea is prepared from the following raw materials in parts by weight: 4 parts by weight of roxburghii, 3 parts by weight of cassia seed, 2 parts by weight of scutellaria baicalensis, 2 parts by weight of scutellaria baicalensis, 3 parts by weight of chrysanthemum rice, 1 part by weight of liquorice, 1 part by weight of jasmine, 1 part by weight of momordica grosvenori, 0.5 part by weight of chrysanthemum, and 1 part by weight of double-petal red rose.

[0053] A preparation method of anoectochilus roxburghii tea comprises the following steps: cleaning each raw material, drying at 40°C for 4 hours, enhancing the aroma, then crushing each raw material into 10-20 mesh sizes, setting the amount of various Chinese medicinal materials according to the above weight ratios through a packaging device after crushing, then feeding them separately and then mixing them, and packaging them into small packages of 5 grams per bag to obtain anoectochilus roxburghii tea bags. Figure 3 Or make the golden thread lotus tea into a cup of tea, such as Figure 4 shown.

[0054] Brewing test: Take a small bag of golden thread lotus tea bag, brew it with 200 ml of 80-90℃ hot water, soak it for 5 minutes and then drink it. It tastes fragrant and sweet, and has obvious health benefits.

[0055] The moisture and ash content of each raw material in the golden thread lotus tea were tested, and the test results showed that the moisture content of angelica sinensis, herba scutellariae, and licorice was ≤8.5%; the moisture content of jasmine, chrysanthemum, chrysanthemum, double red rose, momordica grosvenori, and cassia seed was ≤13%; the moisture content of golden thread lotus was ≤12.0%; and the total ash content of golden thread lotus tea was ≤12.0%.

[0056] Example 2 A roxburghii tea is prepared from the following raw materials in parts by weight: 3 parts by weight of roxburghii, 2 parts by weight of cassia seed, 2 parts by weight of scutellaria baicalensis, 2 parts by weight of scutellaria baicalensis, 1 part by weight of chrysanthemum rice, 1 part by weight of liquorice, 1 part by weight of jasmine, 1 part by weight of momordica grosvenori, 0.6 part by weight of chrysanthemum, and 0.9 part by weight of double-petal red rose.

[0057] A preparation method of anoectochilus roxburghii tea comprises the following steps: cleaning each raw material, drying at 35°C for 5 hours, enhancing aroma, then crushing each raw material into 10-20 mesh sizes, setting the amount of various Chinese medicinal materials according to the above weight ratios through a packaging device after crushing, then feeding them separately and then mixing them, and packaging them into small packages of 5 grams each to obtain anoectochilus roxburghii tea bags.

[0058] Brewing test: Take a small bag of golden thread lotus tea bag and brew it with 200 ml of 100℃ hot water. The taste is richer and the health care effect is further improved.

[0059] It can be seen from Examples 1 and 2 that the roxburghii tea of ​​the present invention can achieve good taste and health benefits under different raw material ratios, and the preparation method is accurate and easy, with good practicality and market promotion value.

[0060] Example 3 A roxburghii tea is prepared from the following raw materials in parts by weight: 3 parts by weight of roxburghii, 3 parts by weight of cassia seed, 2 parts by weight of scutellaria baicalensis, 2 parts by weight of scutellaria baicalensis, 1 part by weight of chrysanthemum rice, 1 part by weight of liquorice, 1 part by weight of jasmine, 1 part by weight of momordica grosvenori, 0.6 part by weight of chrysanthemum, and 0.9 part by weight of double-petal red rose. A method for preparing a roxburghii tea beverage comprises the following steps: cleaning each raw material, drying it at 45°C for 3 hours to enhance the aroma, then crushing each raw material into 10-20 mesh sizes, using a packaging device to set the dosage of various Chinese medicinal materials according to the above-mentioned weight ratios, then feeding them separately and then mixing them, and packaging them into small bags of 5 grams each, thereby obtaining roxburghii tea bags. The roxburghii tea beverage can also be prepared as cup tea. Both the roxburghii tea bags and cup tea can be directly poured into water for brewing, making it convenient to drink.

[0061] Example 4 A production system for a golden thread vine tea beverage, comprising a cleaning device, a drying device, a crushing device, a hopper, a feeding device, and a pressing device; the cleaning device, drying device, crushing device, hopper, feeding device, and pressing device are sequentially connected. The hoppers include 20 independently operating hoppers.

[0062] Example 5 The production system described in Example 4 is used to prepare a method for preparing a golden thread lotus tea, comprising the following steps: adding golden thread lotus, cassia seed, yellow grass, chicken bone grass, chrysanthemum rice, licorice, jasmine, momordica grosvenori, chrysanthemum and double red rose to a cleaning device, cleaning them, then conveying them to a drying device, drying them at 35°C for 5 hours, and then transferring each raw material to a crushing device and crushing them to 10-20 meshes. After crushing, each raw material is respectively transferred to a hopper corresponding to each raw material, and the amount of various Chinese medicinal materials is set according to the above-mentioned weight parts, and then the raw materials are separately discharged through a feeding device and then mixed, and finally packaged into small packages of 5 grams per bag through a pressing device to obtain golden thread lotus tea bags; or the mixed golden thread lotus tea is made into golden thread lotus cup tea, such as Figure 4 shown.

[0063] Experimental example Experimental Example 1 Effect Test Weigh 10 g of the roxburghii tea prepared in Example 1 and place it in a 250 ml round-bottom flask. Soak it in 15 times the volume of distilled water for 1 hour, boil it for 1 hour, and filter the tea bag decoction into a beaker. Add another 10 times the volume of distilled water and boil it for 1 hour. Combine the first and second decoctions, concentrate under reduced pressure, and freeze-dry for 48 hours to obtain the aqueous extract of the roxburghii tea. Aroxburghii was extracted using the same method to obtain the roxburghii aqueous extract.

[0064] Weigh 10 g of the roxburghii tea prepared in Example 1, add 15 times the amount of 95% ethanol, soak for 1 hour, heat and reflux for extraction for 1 hour, filter, add 10 times the amount of 95% ethanol to the medicinal residue, heat and reflux for extraction for 1 hour, and filter; combine the two ethanol solutions, concentrate under reduced pressure, and freeze-dry for 36 hours to obtain the ethanol extract of the roxburghii tea. The same method was used to extract the roxburghii to obtain the roxburghii ethanol extract.

[0065] 1.1. Detection of whether extracts of roxburghii tea and polygonatum ginseng tea can improve macrophage inflammatory responses RAW 264.7 macrophages were selected as inflammatory model cells, and the classic stimulatory molecule LPS was used to induce RAW264.7 macrophages to explore the optimal dosage concentration for inducing their inflammatory response. Figure 1 As shown, treatment with LPS (0.0001-100 μg / mL) for 6 hours increased the mRNA expression levels of the inflammatory factors L-1β, L-6, and TNFα, with the 0.01, 0.1, and 100 μg / mL treatment groups showing the most significant increases. Considering that excessive LPS concentrations can affect cell viability, 0.01 μg / mL was selected as the optimal concentration for inducing an inflammatory model in RAW264.7 macrophages.

[0066] LPS stimulation promotes the expression of inflammatory factors such as IL-1β, IL-6, and TNFα. Simultaneously with LPS stimulation, RAW264.7 macrophages were treated with 200 μg / mL of roxburghii tea extract for 6 hours to measure the transcript levels of these three inflammatory factors. The experimental results demonstrated that roxburghii tea extracts (ATW and ATC) significantly reduced the mRNA expression of IL-1β, IL-6, and TNFα, with the ethanol extract being more effective than the water extract. Similar to the results with roxburghii tea extract, roxburghii extracts (AW and AC) significantly reduced the mRNA levels of IL-1β, IL-6, and TNF-α compared to the LPS group, with the ethanol extract group showing a superior effect compared to the water extract group.

[0067] Macrophages can secrete pro-inflammatory factors such as IL-6, promoting the occurrence of inflammation. In this experiment, the L-6 content in the cell culture medium of the blank group was extremely low. After simple stimulation with 10 ng / ml LPS, the L-6 content in the cell culture medium increased significantly. When the cells were treated with 200 μg / mL of tea extract, the L-6 content in the cell supernatant of the ATC, HGC, and AC groups was significantly reduced compared to the LPS model group, while the IL-6 content in ATW, HGT, and AW did not decrease.

[0068] LPS stimulation increased NO production in RAW 264.7 cells. Figure F shows that compared with the LPS model control group, NO levels in the ethanol extract groups (ATC, HGC, and AC treatment groups) were significantly reduced. Although NO levels in the water extract groups (ATW, HGT, and AW treatment groups) were not significantly reduced compared with the LPS group, there was a trend toward a decrease in NO levels.

[0069] 1.2. Detection of the effect of rosmarinus officinalis tea on preventing macrophage inflammatory response In addition to testing the direct intervention effect of golden thread lotus tea on inflammatory response, its preventive effect on inflammatory response was also tested simultaneously. Figure 2 As shown in the figure, compared with the negative control group, the mRNA expression levels of IL-1β, L-6, and TNFa in the LPS group were significantly increased, proving that the inflammation model was successfully induced. Before LPS induction, cells were treated with 200 μg / mL of the extract for 1 hour, then the old culture medium was discarded and replaced with fresh culture medium containing LPS. After culturing for 6 hours, samples were collected and the mRNA levels of inflammatory indicators in the cells were measured. Figure 2 As shown in the results, compared with the LPS group, pretreatment with roxburghii tea water extract (PATW) and polygonatum ginseng tea alcohol extract (PHGC) did not reduce the mRNA levels of L-1β, IL-6 and TNFa, but pretreatment with roxburghii tea alcohol extract (PATC) and polygonatum ginseng tea water extract (PHGT) significantly reduced the expression of IL-1β and IL-6. As for the expression of TNFa, only PHGT significantly reduced its transcriptional expression.

[0070] The above experimental results show that the roxburghii tea extract of the present invention can significantly reduce the expression of inflammatory factors in macrophages, and the corresponding alcohol extract can also reduce the content of NO produced by inflammatory cells, indicating that roxburghii tea is effective in alleviating inflammation. In addition, pretreatment with the roxburghii tea alcohol extract can also significantly reduce the transcription level of inflammatory factors, indicating that roxburghii tea has a significant effect in preventing chronic inflammation and anti-inflammatory.

[0071] The present invention has been described in detail above with reference to specific embodiments and exemplary examples. However, these descriptions should not be construed as limiting the present invention. Those skilled in the art will appreciate that various equivalent substitutions, modifications, or improvements may be made to the technical solutions and implementations of the present invention without departing from the spirit and scope of the present invention, all of which fall within the scope of the present invention. The scope of protection of the present invention shall be determined by the appended claims.

Claims

1. A roxburghii tea, characterized in that: The anoectochilus roxburghii tea is prepared from the following raw materials in parts by weight: 2-5 weight parts of roxburghii, 1-4 weight parts of cassia seed, 1-3 weight parts of scutellaria baicalensis, 1-3 weight parts of chrysanthemum, 1-4 weight parts of chrysanthemum rice, 0.5-2 weight parts of liquorice, 0.5-2 weight parts of jasmine, 0.5-2 weight parts of momordica grosvenori, 0.3-1 weight parts of chrysanthemum, and 0.5-1.5 weight parts of double red rose.

2. The rosmarinus officinalis tea according to claim 1, characterized in that The anoectochilus roxburghii tea is prepared from the following raw materials in parts by weight: 3-4 weight parts of roxburghii, 2-3 weight parts of cassia seed, 2 weight parts of huanghuangcao, 2 weight parts of jigucao, 1 weight part of chrysanthemum rice, 1 weight part of liquorice, 1 weight part of jasmine, 1 weight part of momordica grosvenori, 0.4-0.7 weight part of chrysanthemum, and 0.7-1.2 weight parts of double red rose.

3. The rosmarinus officinalis tea according to claim 2, characterized in that The anoectochilus roxburghii tea is prepared from the following raw materials in parts by weight: 4 parts by weight of roxburghii, 3 parts by weight of cassia seed, 2 parts by weight of huangcao, 2 parts by weight of jiguca, 3 parts by weight of chrysanthemum rice, 1 part by weight of licorice, 1 part by weight of jasmine, 1 part by weight of momordica grosvenori, 0.5 parts by weight of chrysanthemum, and 1 part by weight of double red rose.

4. The rosmarinus officinalis tea according to claim 2, characterized in that The anoectochilus roxburghii tea is prepared from the following raw materials in parts by weight: 3 parts by weight of roxburghii, 2 parts by weight of cassia seed, 2 parts by weight of huangcao, 2 parts by weight of jiguca, 1 part by weight of chrysanthemum rice, 1 part by weight of liquorice, 1 part by weight of jasmine, 1 part by weight of momordica grosvenori, 0.6 part by weight of chrysanthemum, and 0.9 part by weight of double red rose.

5. A method for preparing the roxburghii tea according to any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: The method comprises the following steps: weighing roxburghii, cassia seed, chrysanthemum herb, chrysanthemum root, liquorice, jasmine flower, momordica grosvenori, chrysanthemum and double-petal red rose, washing, drying, extracting fragrance, crushing and packaging to obtain roxburghii tea.

6. The preparation method according to claim 5, characterized in that The drying conditions are: drying at 35-45°C for 3-5 hours.

7. The preparation method according to claim 5, characterized in that Crush the golden thread lotus, cassia seed, yellow grass, chicken bone grass, chrysanthemum rice, liquorice, jasmine, momordica grosvenori, chrysanthemum and double red rose into 10-30 meshes.

8. A production system for aroectochilus roxburghii tea according to any one of claims 1 to 4, characterized in that: The production system includes a cleaning device, a drying device, a crushing device, a hopper, a feeding device, and a pressing device; the cleaning device, the drying device, the crushing device, the hopper, the feeding device, and the pressing device are connected in sequence.

9. The production system according to claim 8, characterized in that The production system includes 18 to 25 hoppers, each of which is equipped with an independent material discharge control system.

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