Device for accelerating cell resuscitation in batches

Abstract

The invention relates to the technical field of cell resuscitation devices, in particular to a batch acceleration cell resuscitation device which comprises a mounting base, a resuscitation box is arranged on the mounting base, and a batch resuscitation mechanism for simultaneously performing water bath heating on a plurality of cryopreservation tube bodies is arranged in the resuscitation box. A water bath heating mechanism is arranged on the inner side wall of the resuscitation box body, an automatic feeding mechanism is arranged at the upper end of the batch resuscitation mechanism, a resuscitation auxiliary mechanism for disinfecting the outer surfaces of the cryopreservation tubes is arranged at the upper end of the resuscitation box body, and the automatic feeding mechanism comprises a rectangular convex block movably mounted on a mounting disc. The automatic feeding mechanism is arranged, the feeding hopper and the L-shaped pushing plate are matched with each other, the cryopreservation pipe body is always in a horizontal state in the feeding process, liquid nitrogen in the external rotation cryopreservation pipe can automatically flow out before water bath heating, and the cryopreservation pipe can be prevented from exploding in the water bath heating process to a certain extent.

Description

Technical Field

[0001] The invention relates to the technical field of cell resuscitation devices, and in particular to a batch accelerated cell resuscitation device. Background Art

[0002] Cell recovery is the process of restoring frozen in vitro cultures or bioactive materials to room temperature at a certain rewarming rate. Whether it is microorganisms, animal cells, plant cells or organs cultured in vitro, they can be first frozen in liquid nitrogen and then recovered under appropriate temperature conditions.

[0003] However, the resuscitation device in the prior art has a simple structure, and most of them are composed of a water bath heating component and a clamping component. The liquid nitrogen remaining in the externally rotating cryotube cannot be discharged before water bath heating. During heating, it is easy to cause the cryotube to explode due to the large temperature difference between the inside and the outside. Moreover, it is impossible to provide necessary protection for the cryotube. If one of the cryotubes explodes during water bath heating due to the large temperature difference between the inside and the outside, it is easy to damage other cryotubes, thereby causing unnecessary losses. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides a batch accelerated cell recovery device, which solves the technical problems that the recovery device in the prior art has a simple structure, cannot discharge the residual liquid nitrogen in the externally-rotating cryotube before water bath heating, and easily causes the cryotube to explode during heating. The device has the advantage of being able to discharge the liquid nitrogen before water bath heating, which can effectively prevent the cryotube from exploding.

[0005] To solve the above technical problems, the present invention provides the following technical solutions: a batch accelerated cell recovery device, comprising a mounting base, a recovery box is arranged on the mounting base, and a cryopreservation tube body is placed inside the recovery box. A batch recovery mechanism for simultaneously performing water bath heating on multiple cryopreservation tube bodies is arranged inside the recovery box, a water bath heating mechanism is arranged on the inner side wall of the recovery box, an automatic feeding mechanism is arranged on the upper end of the batch recovery mechanism, and a recovery auxiliary mechanism for disinfecting the outer surface of the cryopreservation tube is arranged on the upper end of the recovery box. When cells are recovered, the cryopreservation tube body will enter the interior of the batch recovery mechanism under the action of the automatic feeding mechanism, and then the batch recovery mechanism will transport the cryopreservation tube body to the interior of the water bath heating mechanism for heating and melting. The automatic feeding mechanism includes a movable device mounted on the mounting disc A rectangular protrusion is arranged on the top of the feeding funnel, a pushing chute is provided inside the rectangular protrusion, a feeding funnel is fixedly installed on the rectangular protrusion, an L-shaped pushing plate is movably installed inside the pushing chute, a driving gear is movably installed on the rectangular protrusion, after the cryopreservation tube body enters the interior of the pushing chute, the L-shaped pushing plate will push the cryopreservation tube body horizontally into the interior of the storage bracket, an electromagnet assembly is fixedly installed on the rectangular protrusion, a tube body limiting rod is movably installed on the side of the feeding funnel, one end of the tube body limiting rod is located inside the feeding funnel, the other end of the tube body limiting rod is located outside the feeding funnel and a permanent magnet block is fixedly installed, a reset spring is provided on the outside of the tube body limiting rod, the permanent magnet block has the same magnetic pole as the side facing the electromagnet assembly, when the electromagnet assembly is energized to generate magnetism, the tube body limiting rod will move toward the inside of the feeding funnel.

[0006] Preferably, the batch resuscitation mechanism includes an electric push rod fixedly installed inside the resuscitation box, a mounting disc is movably installed on the upper end of the electric push rod, a driving assembly for rotating the rectangular protrusion is provided on the mounting disc, a plurality of rotating protrusions are arranged on the mounting disc at equal intervals along the circumferential direction, a storage bracket for placing the cryogenic tube body is fixedly installed on the rotating protrusion, a limiting rubber ring is fixedly installed on the inner side of the storage bracket, and a fixed baffle is provided on the mounting disc. When there is a cryogenic tube body on the storage bracket, the rotating protrusion will rotate to a state parallel to the fixed baffle. After the staff takes out the cryogenic tube body, the rotating protrusion will rotate to a state perpendicular to the fixed baffle.

[0007] Preferably, a driving motor for driving the mounting disc to reciprocate is provided inside the upper end of the electric push rod. When the driving motor is powered on, the mounting disc is caused to reciprocate at a certain angle.

[0008] Preferably, the water bath heating mechanism comprises a water bath box which can be detachably mounted inside the resuscitation box, a plurality of partition baffles are arranged at equal intervals inside the water bath box, independent water bath cavities are formed between adjacent partition baffles, circular through holes are penetrated through the partition baffles, a splash-proof shield is fixedly mounted on the outside of the storage bracket, the water bath box is in a circular shape, filled with clean water, the water level is aligned with the middle of the circular through hole, a heating component is provided at the bottom, and the clean water can be quickly heated according to the needs of the staff during use.

[0009] Preferably, the cross-sectional diameter of the splash shield decreases from top to bottom, and the lower end of the splash shield is sleeved on the outside of the storage bracket, which can effectively prevent a large amount of water droplets from splashing onto the tube mouth of the cryotube during the water bath process and causing contamination.

[0010] Preferably, a fixed convex strip is provided at the upper end of the L-shaped push plate, and an avoidance square groove matching the fixed convex strip is opened on the side of the rectangular convex block, and a touch switch is provided inside the avoidance square groove. When the fixed convex strip is located inside the avoidance square groove, the touch switch will be turned on, and when the fixed convex strip is moved out of the avoidance square groove, the touch switch will be automatically disconnected.

[0011] Preferably, the L-shaped pushing plate is meshed with a driving gear, and when the driving gear rotates, the L-shaped pushing plate can move horizontally along the pushing chute, thereby pushing the cryogenic tube outward.

[0012] Preferably, the touch switch is electrically connected to the electromagnet assembly via a wire. When the touch switch is turned on, the electromagnet assembly is energized to generate magnetism, and when the touch switch is turned off, the electromagnet assembly is non-magnetic.

[0013] Preferably, the resuscitation auxiliary mechanism includes a fixed bracket fixedly mounted on the resuscitation box, a movable disc is rotatably connected inside the fixed bracket, a rectangular through groove for avoiding the feeding funnel is provided on the movable disc, an annular enclosure is fixedly mounted on the fixed bracket, a square hole for spraying liquid is provided on the outer side of the annular enclosure, a liquid adding pipe is fixedly connected on the annular enclosure, when the feeding funnel moves up and down with the mounting disc, the movable disc will not interfere with the movement of the feeding funnel due to the presence of the rectangular through groove.

[0014] Preferably, a plurality of the liquid spraying square holes are provided at equal intervals, and a liquid adding pipe is connected to the ethanol storage tank. The alcohol solution in the ethanol storage tank will enter the interior of the annular enclosure through the liquid adding pipe and be sprayed out through the liquid spraying square holes.

[0015] By means of the above technical solution, the present invention provides a device for accelerating cell recovery in batches, which has at least the following beneficial effects:

[0016] 1. The present invention provides an automatic feeding mechanism and utilizes the cooperation between the feeding funnel and the L-shaped pushing plate. The cryotube body is always in a horizontal state during the feeding process, so that the liquid nitrogen in the externally rotating cryotube can automatically flow out before the water bath heating, which can prevent the cryotube from exploding during the water bath heating process to a certain extent.

[0017] 2. The present invention provides an automatic feeding mechanism and utilizes the cooperation between the rectangular protrusion and the driving assembly to automatically place multiple cryotube bodies into the storage rack in sequence, which can effectively reduce the time that the staff is in contact with the cryotubes, thereby improving efficiency and preventing the staff from being frostbitten by liquid nitrogen.

[0018] 3. The present invention sets up an automatic feeding mechanism and utilizes the mutual cooperation between the electromagnet assembly and the tube body limit rod to automatically compress and limit the remaining cryotubes during the horizontal pushing process, thereby preventing the cryotubes from falling prematurely and causing motion interference with the horizontal movement of the L-shaped pushing plate.

[0019] 4. The present invention provides a batch recovery mechanism and utilizes the cooperation between the mounting disc and a plurality of rotating protrusions to perform water bath heating on a plurality of cryotubes at one time, thereby realizing batch processing and effectively improving the efficiency of cell recovery.

[0020] 5. The present invention sets up a batch resuscitation mechanism and utilizes the mutual cooperation between the electric push rod and the mounting disc. The cryotubes can be automatically shaken back and forth during water bath heating to ensure uniform heating. Moreover, the cryotubes can be quickly removed from the water after heating is completed, which can effectively avoid excessive heating and lead to increased cell mortality.

[0021] 6. The present invention provides a water bath heating mechanism and utilizes the cooperation between the partition baffle and the water bath box, so that each cryotube can be placed in an independent water bath cavity during water bath heating. In this way, even if a cryotube explodes due to failure to completely discharge liquid nitrogen, other cryotubes will not be damaged.

[0022] 7. The present invention sets a water bath heating mechanism and utilizes the mutual cooperation between the splash shield and the partition baffle. The partition baffle divides the annular water surface into a plurality of interconnected parts, which can effectively reduce the fluctuation of the water surface caused by shaking the cryotube. In addition, the splash shield can shield and protect the tube mouth of the cryotube, which can effectively prevent the tube mouth of the cryotube from being contaminated by the water in the water bath during the water bath.

[0023] 8. The present invention sets up a resuscitation auxiliary mechanism and utilizes the mutual cooperation between the annular enclosure and the square injection hole to automatically disinfect the outer surface of the cryotube body after heating in a water bath, thereby preventing the cryotube from contaminating the operating table after being transferred to the sterile operating table, and also increasing the success rate of cell resuscitation. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0025] Figure 1 It is a three-dimensional diagram of the overall structure of the present invention;

[0026] Figure 2 It is a schematic diagram of the structure inside the resuscitation box of the present invention;

[0027] Figure 3 It is a structural schematic diagram of the batch resuscitation mechanism in the present invention;

[0028] Figure 4 It is a structural schematic diagram of the automatic feeding mechanism in the present invention;

[0029] Figure 5 It is a structural schematic diagram of the water bath heating mechanism in the present invention;

[0030] Figure 6 It is a structural schematic diagram of the partition baffle in the present invention;

[0031] Figure 7 It is a schematic diagram of the structure of the splash shield in the present invention;

[0032] Figure 8 It is a structural schematic diagram of the L-shaped push plate in the present invention;

[0033] Fig. 9 It is a structural schematic diagram of the material pushing chute in the present invention;

[0034] Fig.10 It is a schematic diagram of the structure of the driving gear in the present invention;

[0035] Fig.11 It is a structural schematic diagram of the tube body limiting rod in the present invention;

[0036] Fig.12 It is a structural schematic diagram of the resuscitation assisting mechanism in the present invention.

[0037] In the figure: 1. Installation base; 2. Recovery box; 3. Batch recovery mechanism; 301. Electric push rod; 302. Installation disc; 303. Rotating convex block; 304. Storage bracket; 305. Limiting rubber ring; 306. Fixed baffle; 4. Cryopreservation tube body; 5. Water bath heating mechanism; 501. Water bath box; 502. Separation baffle; 503. Water bath cavity; 504. Circular through hole; 505. Splash shield; 6. Automatic feeding mechanism; 601. Rectangular convex block; 602 , feeding funnel; 603, driving assembly; 604, pushing chute; 605, L-shaped pushing plate; 606, driving gear; 607, electromagnet assembly; 608, tube limit rod; 609, permanent magnet block; 610, fixed convex strip; 611, avoidance square groove; 612, touch switch; 7, resuscitation auxiliary mechanism; 701, fixed bracket; 702, movable disc; 703, rectangular through groove; 704, annular enclosure; 705, liquid spraying square hole; 706, liquid adding pipeline. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] Embodiment 1

[0040] The resuscitation devices in the prior art have a simple structure, and most of them are composed of a water bath heating component and a clamping component. The liquid nitrogen remaining in the externally rotating cryotube cannot be discharged before the water bath is heated, and the cryotube is easily exploded due to the large temperature difference between the inside and the outside during heating. In order to solve this technical defect in the prior art, Figure 1-Figure 4 as well as Figure 8-Figure 11 As shown, this embodiment proposes a batch accelerated cell recovery device, which can automatically place multiple cryotube bodies 4 into the storage bracket 304 in sequence, which can effectively reduce the time that the staff is in contact with the cryotubes, which can not only improve efficiency but also prevent the staff from being frostbitten by liquid nitrogen. The device is equipped with a recovery box 2 on the mounting base 1, and the cryotube bodies 4 are placed inside the recovery box 2. The recovery box 2 is provided with a batch recovery mechanism 3 for simultaneously performing water bath heating on multiple cryotube bodies 4. The inner side wall of the recovery box 2 is provided with a water bath heating mechanism 5. The upper end of the batch recovery mechanism 3 is provided with an automatic feeding mechanism 6, and the upper end of the recovery box 2 is provided with a recovery auxiliary mechanism 7 for disinfecting the outer surface of the cryotube. When the cells are recovered, the cryotube bodies 4 will enter the interior of the batch recovery mechanism 3 under the action of the automatic feeding mechanism 6. Next, the batch recovery mechanism 3 will transport the cryotube bodies 4 to the interior of the water bath heating mechanism 5 for heating and melting.

[0041] In order to automatically discharge the residual liquid nitrogen in the cryopreservation tube before the water bath, the present embodiment is provided with an automatic feeding mechanism 6. Specifically, the automatic feeding mechanism 6 includes a rectangular protrusion 601 movably mounted on the mounting disc 302, a pushing chute 604 is provided inside the rectangular protrusion 601, a feeding funnel 602 is fixedly mounted on the rectangular protrusion 601, an L-shaped pushing plate 605 is movably mounted inside the pushing chute 604, a driving gear 606 is movably mounted on the rectangular protrusion 601, and the cryopreservation tube body 4 enters the pushing chute 604. 04, the L-shaped pushing plate 605 will push the cryotube body 4 horizontally into the storage bracket 304, and the L-shaped pushing plate 605 is meshed with the driving gear 606. When the driving gear 606 rotates, the L-shaped pushing plate 605 can move horizontally along the pushing chute 604, so as to push the cryotube outward. An electromagnet assembly 607 is fixedly installed on the rectangular protrusion 601, and a tube body limiting rod 608 is movably installed on the side of the feeding funnel 602. One end of the tube body limiting rod 608 is located inside the feeding funnel 602. The other end of the tube limit rod 608 is located outside the feeding funnel 602 and is fixedly installed with a permanent magnet block 609. A reset spring is sleeved on the outside of the tube limit rod 608. The permanent magnet block 609 has the same magnetic pole as the electromagnet assembly 607. When the electromagnet assembly 607 is energized to generate magnetism, the tube limit rod 608 moves toward the inside of the feeding funnel 602. The upper end of the L-shaped push plate 605 is provided with a fixed convex strip 610, and the side of the rectangular convex block 601 is provided with a matching fixed convex strip 610. The avoidance square groove 611 has a touch switch 612 disposed inside. When the fixed convex strip 601 is located inside the avoidance square groove 611, the touch switch 612 is turned on. When the fixed convex strip 601 is moved out of the avoidance square groove 611, the touch switch 612 is automatically disconnected. The touch switch 612 is electrically connected to the electromagnet assembly 607 through a wire. When the touch switch 612 is turned on, the electromagnet assembly 607 is energized to generate magnetism. When the touch switch 612 is disconnected, the electromagnet assembly 607 is non-magnetic.

[0042] According to the above content, during the cell recovery process, the staff will sequentially place multiple cryopreservation tubes into the feeding funnel 602 . At this time, the cryopreservation tube placed first will fall into the pushing chute 604 .

[0043] Next, if Figure 3 As shown, the L-shaped pushing plate 605 will move horizontally along the pushing chute 604 under the action of the driving gear 606, and the L-shaped pushing plate 605 will push the cryotube into the interior of the limiting rubber ring 305 when moving horizontally.

[0044] Moreover, during the movement of the L-shaped pushing plate 605, the fixed protrusion 610 will enter the inside of the avoidance square groove 611. At this time, the touch switch 612 will be in the on state. Next, the electromagnet assembly 607 will generate magnetism to move the tube body limiting rod 608 toward the inside of the feeding funnel 602, thereby pressing and limiting the frozen storage tube above the pushing chute 604, so as to avoid the frozen storage tube above interfering with the movement of the L-shaped pushing plate 605 during the pushing process.

[0045] After a pushing operation is completed, the L-shaped pushing plate 605 will move in the opposite direction under the action of the driving gear 606. When the fixed protrusion 610 is completely moved out from the inside of the avoidance square groove 611, the electromagnet assembly 607 will lose its magnetism. Next, the tube body limiting rod 608 will move outward under the action of the reset spring, thereby releasing the limiting effect on the cryogenic tube. Subsequently, the cryogenic tube located above the pushing chute 604 will fall into the inside of the pushing chute 604.

[0046] Next, the rectangular protrusion 601 will rotate a certain angle under the action of the driving component 603, so that the opening of the pushing chute 604 is aligned with the next storage bracket 304. Then, the above pushing process is repeated until all the cryopreservation tubes inside the feeding funnel 602 are inserted into the corresponding storage bracket 304.

[0047] The present embodiment sets an automatic feeding mechanism 6, and utilizes the mutual cooperation between the rectangular protrusion 601 and the driving assembly 603, so that multiple cryotube bodies 4 can be automatically placed in the storage bracket 304 in sequence, which can effectively reduce the time that the staff is in contact with the cryotubes, which can not only improve efficiency but also prevent the staff from being frostbitten by liquid nitrogen; moreover, the present embodiment sets an automatic feeding mechanism 6, and utilizes the mutual cooperation between the feeding funnel 602 and the L-shaped pushing plate 605, so that the cryotube body 4 is always in a horizontal state during the feeding process, which can make the liquid nitrogen in the external rotating cryotube automatically flow out before the water bath heating, and can prevent the cryotube from exploding during the water bath heating process to a certain extent; in addition, the present embodiment sets an automatic feeding mechanism 6, and utilizes the mutual cooperation between the electromagnet assembly 607 and the tube body limiting rod 608, so that the remaining cryotubes can be automatically pressed and limited during the horizontal pushing process, so as to avoid the cryotubes from falling off prematurely and causing motion interference to the horizontal movement of the L-shaped pushing plate 605.

[0048] Embodiment 2

[0049] In order to improve the efficiency of cell recovery as much as possible and realize batch processing, based on Example 1, Figure 1-Figure 4 as well as Figure 7As shown, the present embodiment is provided with a batch recovery mechanism 3. Specifically, the batch recovery mechanism 3 comprises an electric push rod 301 fixedly mounted inside the recovery box 2. A mounting disc 302 is movably mounted on the upper end of the electric push rod 301. A driving motor for driving the mounting disc 302 to reciprocate is arranged inside the upper end of the electric push rod 301. When the driving motor is powered on, the mounting disc 302 is caused to reciprocate at a certain angle. A driving component 603 for rotating a rectangular protrusion 601 is arranged on the mounting disc 302. The mounting disc 302 is provided with a driving component 603 for rotating a rectangular protrusion 601. A plurality of rotating protrusions 303 are arranged at equal intervals in the circumferential direction, and a storage bracket 304 for placing the cryotube body 4 is fixedly installed on the rotating protrusion 303. A limiting rubber ring 305 is fixedly installed on the inner side of the storage bracket 304, and a fixed baffle 306 is arranged on the mounting disk 302. When there is a cryotube body 4 on the storage bracket 304, the rotating protrusion 303 will rotate to a state parallel to the fixed baffle 306. After the staff takes out the cryotube body 4, the rotating protrusion 303 will rotate to a state perpendicular to the fixed baffle 306.

[0050] According to the above content, if Figure 3 As shown, initially, the rotating protrusion 303 is in a vertical state. Next, the cryotube body 4 is horizontally inserted into the storage bracket 304 under the action of the automatic feeding mechanism 6. Subsequently, the rotating protrusion 303 automatically rotates to a state parallel to the fixed baffle 306.

[0051] Subsequently, the electric push rod 301 will automatically contract to move the mounting disc 302 vertically downward. When the mounting disc 302 moves downward, the cryotube body 4 will enter the interior of the water bath 501. At the same time, the mounting disc 302 will reciprocate under the action of the driving motor (the reciprocating rotation angle is 25 degrees), so that the cryotube body 4 is in a reciprocating swinging state to ensure uniform heating.

[0052] After the water bath is heated for a certain period of time, the mounting disc 302 will move upward under the action of the electric push rod 301, so that the cryotube body 4 can be quickly moved out of the water bath 501, thereby avoiding an increase in cell mortality due to a long water bath time.

[0053] In this embodiment, a batch recovery mechanism 3 is provided, and the cooperation between the mounting disk 302 and the plurality of rotating protrusions 303 is utilized to perform water bath heating on a plurality of cryotubes at one time, thereby realizing batch processing and effectively improving the efficiency of cell recovery. In addition, in this embodiment, a batch recovery mechanism 3 is provided, and the cooperation between the electric push rod 301 and the mounting disk 302 is utilized to automatically shake the cryotubes back and forth during water bath heating to ensure uniform heating, and the cryotubes are quickly removed from the water after heating is completed, thereby effectively avoiding excessive heating and causing an increase in cell mortality.

[0054] Embodiment 3

[0055] In order to prevent the cryopreservation tube from being contaminated during the water bath heating process, based on the above embodiment, Figure 2 as well as Figure 5-Figure 7 As shown, the present embodiment is provided with a water bath heating mechanism 5. Specifically, the water bath heating mechanism 5 comprises a water bath box 501 which is detachably mounted inside the resuscitation box body 2. A plurality of partition baffles 502 are arranged at equal intervals inside the water bath box 501. Independent water bath cavities 503 are formed between adjacent partition baffles 502. Circular through holes 504 are penetrated through the partition baffles 502. A splash shield 505 is fixedly mounted on the outer side of the storage bracket 304. The water bath box 501 is in an annular shape and is filled with clean water. The water level is aligned with the middle of the circular through hole 504. A heating component is arranged at the bottom. When in use, the clean water can be quickly heated according to the needs of the staff. The cross-sectional diameter of the splash shield 505 decreases from top to bottom. The lower end of the splash shield 505 is sleeved on the outside of the storage bracket 304, which can effectively prevent a large amount of water droplets from splashing onto the nozzle of the cryopreservation tube during the water bath and causing contamination.

[0056] According to the above content, during the cell recovery process, the cryotube body 4 will move to the inside of the water bath 501 under the action of the electric push rod 301, and then the cryotube body 4 will follow the mounting disc 302 to rock back and forth.

[0057] like Figure 5 As shown, after the cryotubes enter the water bath 501, each cryotube will be located inside a water bath cavity 503. Next, the cryotubes will be evenly heated inside the water bath cavity 503 and will be melted quickly.

[0058] Moreover, if Figure 7 As shown, the outer sleeve of the storage bracket 304 is provided with a splash-proof shield 505, which can effectively prevent the tube mouth of the cryotube from contacting with water, thereby avoiding contamination of the tube mouth.

[0059] In this embodiment, a water bath heating mechanism 5 is provided, and the cooperation between the partition baffle 502 and the water bath box 501 is utilized. When the water bath is heated, each cryotube can be placed in an independent water bath cavity 503. In this way, even if a cryotube explodes due to failure to completely discharge the liquid nitrogen, other cryotubes will not be damaged. Moreover, in this embodiment, a water bath heating mechanism 5 is provided, and the cooperation between the splash shield 505 and the partition baffle 502 is utilized. The partition baffle 502 divides the annular water surface into a plurality of interconnected parts, which can effectively reduce the fluctuation of the water surface caused by shaking the cryotube. In addition, the splash shield 505 can shield and protect the tube mouth of the cryotube, which can effectively prevent the tube mouth of the cryotube from being contaminated by the water in the water bath box 501 during the water bath.

[0060] Embodiment 4

[0061] In order to prevent the freezing tubes heated in a water bath from contaminating the sterile operating table, based on the above embodiment, this embodiment is provided with a resuscitation auxiliary mechanism 7. Specifically, the resuscitation auxiliary mechanism 7 includes a fixed bracket 701 fixedly mounted on the resuscitation box 2, the fixed bracket 701 is internally rotatably connected with a movable disc 702, the movable disc 702 is provided with a rectangular through groove 703 for avoiding the feeding funnel 602, and the fixed bracket 701 is fixedly mounted with an annular enclosure 704, the outer side of the annular enclosure 704 is opened. A liquid spraying square hole 705 is provided, and a liquid adding pipe 706 is fixedly connected to the annular enclosure 704. When the feeding funnel 602 moves up and down following the mounting disc 302, due to the presence of the rectangular through groove 703, the movable disc 702 will not interfere with the movement of the feeding funnel 602. A plurality of liquid spraying square holes 705 are provided at equal intervals. The liquid adding pipe 706 is connected to the ethanol storage tank. The alcohol solution in the ethanol storage tank will enter the interior of the annular enclosure 704 through the liquid adding pipe 706 and be sprayed out through the liquid spraying square holes 705.

[0062] According to the above content, after the water bath heating is completed, the mounting disc 302 will move upward under the action of the electric push rod 301, thereby transferring the cryotube body 4 to the upper end of the recovery box 2.

[0063] When the cryopreservation tube moves to the top of the recovery box 2, the ethanol solution (70% ethanol) inside the liquid adding pipe 706 will be sprayed outward through the multiple square spray holes 705, thereby automatically disinfecting the outside of the cryopreservation tube.

[0064] This embodiment provides a resuscitation auxiliary mechanism 7, and utilizes the cooperation between the annular enclosure 704 and the liquid spraying square hole 705 to automatically disinfect the outer surface of the cryotube body 4 after heating in a water bath, thereby preventing the cryotube from contaminating the operating table after being transferred to the sterile operating table, and also increasing the success rate of cell resuscitation.

[0065] The control method of the present invention is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by technicians in this field. The provision of power is also common knowledge in this field. The present invention is mainly used to protect mechanical devices, so the present invention will no longer explain the control method and circuit connection in detail.

[0066] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0067] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A batch accelerated cell recovery device, comprising a mounting base (1), a recovery box (2) being arranged on the mounting base (1), a cryotube body (4) being placed inside the recovery box (2), characterized in that: The resuscitation box (2) is provided with a batch resuscitation mechanism (3) for simultaneously heating a plurality of cryotube bodies (4) in a water bath, the inner side wall of the resuscitation box (2) is provided with a water bath heating mechanism (5), the upper end of the batch resuscitation mechanism (3) is provided with an automatic feeding mechanism (6), and the upper end of the resuscitation box (2) is provided with a resuscitation auxiliary mechanism (7) for disinfecting the outer surface of the cryotube; The batch recovery mechanism (3) comprises an electric push rod (301) fixedly mounted inside the recovery box (2), a mounting disc (302) being movably mounted on the upper end of the electric push rod (301), an automatic feeding mechanism (6) comprising a rectangular convex block (601) movably mounted on the mounting disc (302), a driving assembly (603) for rotating the rectangular convex block (601) being provided on the mounting disc (302), a material pushing chute (604) being provided inside the rectangular convex block (601), a material feeding funnel (602) being fixedly mounted on the rectangular convex block (601), and a material pushing chute (604) being provided inside the rectangular convex block (601). 04) is movably installed with an L-shaped push plate (605), a driving gear (606) is movably installed on the rectangular protrusion (601), an electromagnet assembly (607) is fixedly installed on the rectangular protrusion (601), a tube body limiting rod (608) is movably installed on the side of the feeding funnel (602), one end of the tube body limiting rod (608) is located inside the feeding funnel (602), the other end of the tube body limiting rod (608) is located outside the feeding funnel (602) and is fixedly installed with a permanent magnet block (609), and a reset spring is sleeved on the outside of the tube body limiting rod (608).

2. The device for accelerating cell recovery in batches according to claim 1, characterized in that: A plurality of rotating protrusions (303) are arranged at equal intervals along the circumferential direction on the mounting disc (302); a storage bracket (304) for placing the cryotube body (4) is fixedly mounted on the rotating protrusion (303); a limiting rubber ring (305) is fixedly mounted on the inner side of the storage bracket (304); and a fixed baffle (306) is arranged on the mounting disc (302).

3. The device for accelerating cell recovery in batches according to claim 2, characterized in that: A driving motor for driving the mounting disc (302) to reciprocate is disposed inside the upper end of the electric push rod (301).

4. The device for accelerating cell recovery in batches according to claim 1, characterized in that: The water bath heating mechanism (5) comprises a water bath box (501) which is detachably mounted inside the resuscitation box (2); a plurality of partition baffles (502) are arranged at equal intervals inside the water bath box (501); mutually independent water bath cavities (503) are formed between adjacent partition baffles (502); circular through holes (504) are formed through the partition baffles (502); and a splash shield (505) is fixedly mounted on the outer side of the storage bracket (304).

5. The device for accelerating cell recovery in batches according to claim 4, characterized in that: The cross-sectional diameter of the splash shield (505) decreases from top to bottom, and the lower end of the splash shield (505) is sleeved on the outside of the storage bracket (304).

6. The device for accelerating cell resuscitation in batches according to claim 1, characterized in that: The upper end of the L-shaped push plate (605) is provided with a fixed convex strip (610), the side of the rectangular convex block (601) is provided with an avoidance square groove (611) matching the fixed convex strip (610), and a touch switch (612) is arranged inside the avoidance square groove (611).

7. The device for accelerating cell recovery in batches according to claim 1, characterized in that: The L-shaped push plate (605) is meshed with the driving gear (606).

8. The device for accelerating cell recovery in batches according to claim 6, characterized in that: The touch switch (612) is electrically connected to the electromagnet assembly (607) via a wire.

9. The device for accelerating cell resuscitation in batches according to claim 1, characterized in that: The resuscitation auxiliary mechanism (7) comprises a fixed bracket (701) fixedly mounted on the resuscitation box (2), the fixed bracket (701) being internally rotatably connected to a movable disc (702), the movable disc (702) being provided with a rectangular through slot (703) for evading a feeding funnel (602), an annular enclosure (704) being fixedly mounted on the fixed bracket (701), a liquid spraying square hole (705) being provided on the outer side of the annular enclosure (704), and a liquid adding pipeline (706) being fixedly connected to the annular enclosure (704).

10. The device for accelerating cell recovery in batches according to claim 9, characterized in that: A plurality of liquid spraying square holes (705) are provided at equal intervals, and a liquid adding pipeline (706) is connected to an ethanol storage tank.

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