Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Low-temperature lipase mycopremna, low-temperature lipase and method of preparing the same

A production method, lipase technology, applied in the direction of fungi, hydrolytic enzymes, etc.

Inactive Publication Date: 2008-02-27
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the low-temperature lipase studied is mainly produced by cold-tolerant microbial strains, and there are few strains available for research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Low-temperature lipase mycopremna, low-temperature lipase and method of preparing the same
  • Low-temperature lipase mycopremna, low-temperature lipase and method of preparing the same
  • Low-temperature lipase mycopremna, low-temperature lipase and method of preparing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1: the cultivation-I of low-temperature lipase producing bacteria (GL-1)

[0074] The low-temperature lipase producing bacteria GL-1 of the present invention is inoculated in the fat assimilation plate medium, wherein (NH 4 ) 2 SO 4 0.1%;K 2 HPO 4 0.1%; NaCl0.05%; MgSO 4 ·7H 2 O 0.05%; FeSO 4 ·7H 2 O 0.001%; ​​yeast extract 0.5%; agar 2.5%; natural pH; take olive oil and 2% polyvinyl alcohol (PVA) to mix in a ratio of 1:3, 10000r / min stirring and emulsifying for 5min, 0.8kg / cm 2 After sterilizing for 30 minutes, take 12ml and add it to 100ml of the above medium. After culturing at about 20°C for 5-7 days, a transparent circle can be formed around the colony of the low-temperature lipase-producing bacteria of the present invention.

Embodiment 2

[0075] Embodiment 2: Cultivation-II of low-temperature lipase-producing bacteria (GL-1)

[0076] Rhodamine B was prepared as a 0.05% mother solution, and added to the oil assimilation medium of Example 1 in an amount of 1.0%, to prepare a Rhodamine B chromogenic medium. When the low-temperature lipase-producing bacteria GL-1 of the present invention is inoculated in the above-mentioned culture medium, an orange-red halo will appear around the colonies of the low-temperature lipase-producing bacteria of the present invention, and red fluorescent circles will appear under ultraviolet light.

Embodiment 3

[0077] Embodiment 3: the fermentation culture of low-temperature lipase producing bacteria (GL-1 bacterial strain)

[0078] Insert the low-temperature lipase-producing bacteria of the present invention into the wort liquid seed medium, wherein malt extract powder 0.3; yeast extract 0.3; peptone 0.5; glucose 1.0; 1.0kg / cm 2 Steam sterilize for 15 minutes. After culturing at 20°C for 1-2 days, inoculate in the following fermentation medium with a 5% inoculation amount. Bean cake powder 2.0, corn syrup 2.0, glucose 1.0, K 2 HPO 4 0.5, NaNO 3 0.5, pH7.0, in 250ml Erlenmeyer flask, 50ml per bottle, 0.8kg / cm 2 Steam sterilize for 3O minutes. Shake culture at 20°C and 200rpm for 48h. After culturing, the bacterium is removed by centrifugation to obtain a low-temperature lipase crude enzyme liquid, and the fermented enzyme yield can reach 20 U / mL. Add ammonium sulfate to the lipase solution obtained in this way to make its saturation reach 60%, let it stand for precipitation to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a manufacturing method of low-temperature lipase through Trichosporon pullulans, which is characterized by the following: utilizing Trichosporon pullulans (CGMCC. No. 1674) to build bacterial preservation, rejuvenated and breeding system; optimizing the sieving through culture medium formula; controlling the fermenting technique; manufacturing the product with good low-temperatur activity and high-enzyme activity; fitting for washing, edible manufacturing, leather manufacturing, help digest agent, organic synthetic chemical product, perfume, drug preparation, fat manufacturing domain widely.

Description

technical field [0001] The invention relates to a low-temperature lipase, a bacteria producing the enzyme and a production method thereof. Specifically, the present invention relates to a lipase produced by low-temperature-resistant microbial strains and having higher activity at lower temperatures, and a production method for producing low-temperature lipase using the microbial strains. technical background [0002] Lipase is an enzyme ubiquitous in nature. According to its different sources, it can be divided into three categories: animal lipase, plant lipase and microbial lipase. Among them, microbial lipase is widely used in washing, food processing, leather processing, digestive aids, organic synthetic chemical products, spices, and pharmaceutical preparations because of its easy production, easy expansion, and its specific decomposition characteristics for fatty substances. , oil processing and other aspects have been widely used, becoming one of the world's main ind...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N9/20
Inventor 茆军冯怀蓉张志东顾美英唐琦勇
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com