Low-temperature alkaline proteinase marine bacteria strain, low-temperature alkaline proteinase and production method thereof

A low-temperature protease and production method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as large differences and differences in enzyme characteristics, and achieve high enzyme activity, high and low temperature stability, and convenience Collection of refined effects
CN102120974AInactive Publication Date: 2011-07-13ZHEJIANG WANLI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG WANLI UNIV
Publication Date
2011-07-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a marine Pseudoalteromonas sp. B8453013 (strain collection number CGMCC No.3281) of low-temperature proteinase, a production method for producing low-temperature proteinase from the strain and a low-temperature proteinase. A system method for strain collection, rejuvenation and seed selection is built by alternating Pseudoalteromonas sp. B8453013; the method for producing the low-temperature proteinase is built by culture medium optimization screening and the optimized control of fermentation technology conditions; and the produced low-temperature proteinase has the advantages of good low-temperature activity and high enzyme activity. The low-temperature proteinase can be widely applied to industries, such as detergents, feed, fur, food processing and the like.
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Description

technical field

[0001] The invention relates to the field of microorganisms and microbial fermentation, in particular to a protease produced by bacteria and having a low optimum enzyme reaction temperature, a microbial strain producing the enzyme and a method for producing the enzyme. Background technique

[0002] Protease is currently the most widely used enzyme, accounting for 60-65% of the global enzyme industry market share. The proteases currently used are basically mesophilic enzymes. Compared with mesophilic protease, low temperature protease can maintain activity at low temperature (<10°C), K cat value and physiological efficiency (K cat / K m ) is high and has higher catalytic activity and other characteristics, so low-temperature protease has more application value than medium-temperature protease. Low-temperature protease is used in food, detergent, cosmetics, aquatic feed and other industries, and has the advantages that medium-temperature protease cannot r...

Claims

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