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Composite microbial preparation and its application in prevention and treatment of phytophthora

A technology of compound microorganisms and preparations, applied in the field of microorganisms, can solve the problems of slow drug effect and poor control effect, and achieve the effects of reducing fermentation costs, convenient use and reasonable compatibility

Active Publication Date: 2021-04-02
山东土木启生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the defects of poor control effect and slow drug effect of single biocontrol agent in the prior art, the present invention provides a compound microbial preparation and its use in preventing and treating Phytophthora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A compound microbial preparation, which includes Trichosporium yeast, Trichoderma konshii, Bacillus subtilis and Rhodopseudomonas palustris.

[0034] Specifically, the composite microbial preparation is prepared according to the following process:

[0035] Streak inoculation of Trichosporium yeast on YPDA medium and culture to obtain a single colony; pick a single colony and inoculate it on YPD liquid medium for culture, culture at 30°C for 24 hours, and obtain seed liquid for use;

[0036] Formula of YPD medium: glucose 20g, peptone 20g, yeast extract powder 10g, add distilled water to 1000mL;

[0037] Formula of YPDA medium: glucose 20g, peptone 20g, yeast extract powder 10g, agar 20g, add distilled water to 1000mL;

[0038] Streak inoculation of Trichoderma konshii on the PDA medium and culture to obtain a single colony; pick a single colony and inoculate it on the PDA liquid medium for culture, culture at 30°C, 200rpm shaker for 36 hours, and obtain the seed liquid...

Embodiment 2

[0045] A compound microbial preparation, which includes Trichosporium yeast, Trichoderma konshii, Bacillus subtilis and Rhodopseudomonas palustris.

[0046] Specifically, the composite microbial preparation is prepared according to the following process:

[0047] Streak inoculation of Trichosporium yeast on YPDA medium and culture to obtain a single colony; pick a single colony and inoculate it on YPD liquid medium for culture, culture at 30°C for 24 hours, and obtain seed liquid for use;

[0048] Formula of YPD medium: glucose 20g, peptone 20g, yeast extract powder 10g, add distilled water to 1000mL;

[0049] Formula of YPDA medium: glucose 20g, peptone 20g, yeast extract powder 10g, agar 20g, add distilled water to 1000mL;

[0050] Streak inoculation of Trichoderma konshii on the PDA medium and culture to obtain a single colony; pick a single colony and inoculate it on the PDA liquid medium for culture, culture at 30°C, 200rpm shaker for 36 hours, and obtain the seed liquid...

Embodiment 3

[0057] Control effect of compound microbial preparation of the present invention on capsicum phytophthora

[0058] Groups: Experimental group: Example 1; Control group 1: only use the Trichoderma konshii-Tychosporium mixed fermentation liquid, the rest are the same as in Example 1; Control group 2: Only use Rhodopseudomonas palustris fermentation liquid and subtilis Bacillus fermentation broth, the rest are the same as in Example 1; control group 3: Trichoderma konshii and Trichosporium are fermented separately, and then the four fermentation broths are mixed, and the rest are the same as in Example 1; Blank control: the same amount of clear water.

[0059] Preparation pretreatment: add the preparation to 100 times the weight of water, stir evenly, and set aside.

[0060] Soil treatment: after Phytophthora capsici was cultured in solid for 4 days, it was inserted into V8 liquid medium, cultured at 25°C for 4 days, the liquid medium was poured out, the hyphae were weighed, and ...

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Abstract

The invention belongs to the technical field of microorganisms, and discloses a composite microbial preparation which is prepared by the following process: mixing trichoderma koningii-Trichosporon mixed fermentation liquor, rhodopseudomonas palustris fermentation liquor and bacillus subtilis fermentation liquor uniformly according to the volume ratio of 5-8:2-3:2-3, and then performing vacuum freeze drying to obtain bacterial powder, thereby the composite microbial preparation is obtained. According to the invention, four biocontrol strains are adopted in the composite microbial preparation, which can achieve synergistic symbiosis, have reasonable compatibility, and are not antagonistic to each another; the composite microbial preparation can greatly improve the effect of preventing and treating phytophthora, is harmless to people, livestock and crops, is environmentally friendly, has relatively simple preparation process, is convenient and fast to use and has wide application prospect.

Description

[0001] cross reference [0002] This application claims the priority of the Chinese patent application No. 2018102802880 filed on April 2, 2018 with the title of "a composite microbial preparation and its use in the prevention and treatment of Phytophthora", the entire disclosure content of which is cited herein. technical field [0003] The invention belongs to the technical field of microorganisms, and in particular relates to a composite microbial preparation and its application in preventing and treating Phytophthora. Background technique [0004] Phytophthora (Phytophthora), Peronosporales (Peronosporales) pathogenic bacteria, the lower sporangium and mycelium have no obvious difference, the higher ones are different, the sporangia is lemon-shaped or ovoid, with papillae at the top, causing potato Late blight, taro blight, etc. Hyphae without septa and multinucleate at first, branches are mostly acute-angled, often constricted at the branch, 2-10 μm thick, a few specie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/16C12N1/20A01N63/32A01N63/27A01N63/22A01P3/00C12R1/01C12R1/125C12R1/885C12R1/645
CPCC12N1/14C12N1/16C12N1/20A01N63/32A01N63/27A01N63/22Y02A50/30
Inventor 叶志坚周竞夫齐晔朱锡杭朱增湖
Owner 山东土木启生物科技有限公司
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