73 results about "Rhodopseudomonas sp." patented technology

The invention relates to a complex microorganism additive as well as a preparation method and application thereof, belonging to the biological material technical field of application of a microecological modulator in cultivating domestic animals. The preparation method thereof comprises the following steps: taking 30-60% of Saccharomyces cervisiae, 5-30% of Candida utilis, 5-30% of Rhodopseudom onas Palustris, 5-30% of Lactobacillus plantarum, 5-40% of Leatobacillus acidophilus and 5-25% of Bacillus subtilis; and sequentially carrying out primary bacteria strain culture, secondary fermentation and tertiary fermentation on the bacteria strains so that the bacterial number in fermentation liquor reaches 8-10*108/mL, thus obtaining the complex microorganism additive 808. Long-term on-site experiments prove that the product has reasonable formula, stable efficacy, disease prevention and resistance, improved quality and capability of promoting growth of animals and improving yield of animal products, survival rate and feed conversion rate.

The invention relates to the technical field of microbe application, specifically relates to a microbial agent and application thereof to saline-alkali land soil improvement. The microbial agent is prepared by compounding bacillus amyloliquefaciens Bn-21, Bacillus subtilis Bn-3,Bacillus pumilus Bn-19, Rhodopseudomonas palustris Bn-2 and lactobacillus plantarum Bn-23, the compounded multiple microbes has different functions, each microbe carries out different microbe metabolic activity, every two bacterial strains have no antagonism, the function of each microbe is relatively stable, can play a different role in a soil conditioner, increase microbe diversity in soil, enhance activity of the microbe in soil, strengthen the effects of the soil conditioner for improving soil physical and chemical properties and improving the soil fertility.

The invention discloses an environmentally-friendly composite microorganism preparation for eliminating house-fitment pollution. The composite microorganism preparation comprises the following bacterial components in percentage by weight: 15 to 35 percent of lactobacillus acidophilus, 15 to 35 percent of candida utilis, 5 to 25 percent of bacillus licheniformis, 10 to 25 percent of streptomyces albulus and 15 to 30 percent of rhodopseudomonas palustris and is obtained by compositely culturing the bacterial components by a fermentation process which combines anaerobic fermentation with aerobic fermentation.

The invention aims to provide a production method for a compound microbial preparation for aquatic products. The production method comprises the following steps: respectively carrying out high-density liquid fermentation on strains of bacillus cereus, bacillus licheniformis, halophilic pseudomonas, lactobacillus acidophilus, rhodococcus erythropolis, rhodopseudomonas palustris and enterococcus faecium to obtain ultra-concentrated bacterium powder; and then preparing according to a ratio to obtain the compound microbial preparation. The production process is simple in process, is applicable to most existing fermentation processes and equipment and is good for being popularized and applied in a large scale.

The invention relates to a composite microbial preparation for promoting the growth of rape and a preparation method thereof. The composite microbial preparation for promoting the growth of the rape is characterized by comprising 20-40 parts by weight of strain culturing substance and 60-80 parts by weight of microbial carrier, wherein the strain culturing substance comprises the following raw materials in parts by weight: 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium, 5-10 parts of rhodopseudomonas palustris and 1-4 parts of azotobacter chroococcum. The preparation method of the composite microbial preparation for promoting the growth of the rape comprises the following steps: culturing 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium and the rhodopseudomonas palustris together, culturing the azotobacter chroococcum independently, mixing and fermenting. The invention has the advantages of promoting the growth and the propagation of beneficial microorganisms, increasing the absorption of the root system of the rape to nitrogen, phosphorus, potassium and other organic substances and promoting the normal development of the root system, thus promoting the growth of the rape and reducing the applied chemical fertilizer.

The invention provides a compound microorganism preparation. The compound microorganism preparation is composed of Bacillus cereus, bacillus licheniformis, halophilic pseudomonades, Lactobacillus acidophilus, Rhodococcus erythropolis, Rhodopseudomonas palustris and enterococcus faecium. The viable count of the compound microorganism preparation is as high as 8*1011CFU/g, and the compound microorganism preparation can be widely applied to the aquaculture water areas of fresh water and seawater. Ammonia nitrogen and nitrite in the water can be effectively degraded and water blooms can be restrained under the synergistic effect of the bacterial strains, therefore, the aquatic water can be purified, growing environment for breeding animals can be improved, and productivity can be improved.

The invention provides a microbial preparation for purifying air. By utilizing the synergic effects of streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium, the microbial preparation is capable of effectively reducing concentration of stink comprising ammonia gas, hydrogen sulfide and the like in the air at toilets, culturing farms, dumps and the like, and inhibiting stink-producing elements, so that air quality and human living environment can be substantially improved. A production method provided by the invention is simple, is applicable to majority of conventional fermentation technologies and equipment, and is suitable for large-scale popularization and application.

The invention discloses a microbial preparation for resisting a stress reaction of a prawn. The microbial preparation comprises a compound microbial bacterium, wherein the compound microbial bacterium comprises the following bacteria in percentage by weight: 20-60 percent of bacillus subtilis, 10-50 percent of beer yeast, 3-40 percent of lactobacillus casei and 10-50 percent of rhodopseudomonas palustris. The invention further discloses a preparation method of the microbial preparation. The method comprises the following steps: respectively inoculating the four bacterial strains in corresponding culture media for enrichment culture to obtain bacteria; proportionally mixing the bacteria to obtain a compound microbial bacterium stock solution, and inoculating the compound microbial bacterium stock solution for fermentation to obtain the needed microbial preparation. The invention further discloses the application of the microbial preparation to any one prawn feed. Hydroxyl free radicals in the body of the prawn can be quickly removed by virtue of the microbial preparation, and the concentration of cortisol and the concentration of blood sugar in a serum are reduced, so that the influence of the stress reaction of the prawn on an organism is reduced, and the resistance of the prawn is improved.

The invention provides a culture method facilitating the increase of the growth rate of chlorella pyrenoidosa and cell density and the promotion of fat accumulation. Mixed bacterial fermentation liquid with inactivated Rhodobacter sp. and Rhodopseudomonas sp. is added in a culture medium for culturing chlorella pyrenoidosa. Through the adoption of the culture method, chlorella pyrenoidosa cells can grow quickly, and cultured chlorella pyrenoidosa is high in cell intensity; in particular, by adding the mixed bacterial liquid, the quick multiplication of the chlorella pyrenoidosa cells is promoted, the concentration of the chlorella pyrenoidosa cells is increased, the content of fat is obviously increased, and the method is suitable for mass and large-scale culture production.

The invention discloses a composite microbial deodorizer and a preparation method thereof. According to the invention, thiobacillus ferrooxidans, rhizobium sludge, bacillus amyloliquefaciens, rhodopseudomonas palustris, pseudomonas stutzeri, bacillus coagulans and hansenula polymorpha are selected for use; liquid fermentation is performed respectively, and then compounding is performed in proportion; then linalool is added in proportion and the substances are fully mixed to prepare a composite microbial deodorizer capable of effectively expelling flies and insects, inhibiting growth and propagation of harmful flora and reducing and removing foul gas in the environment. The thiobacillus ferrooxidans (CFU/g) in the product is greater than or equal to 2.95*10<8>; the rhizobium sludge (CFU/g)is greater than or equal to 1.05*10<8>; the bacillus amyloliquefaciens (CFU/g) is greater than or equal to 1.73*10<8>; the rhodopseudomonas palustris (CFU/g) is larger than or equal to 2.55*10<8>; thepseudomonas stutzeri (CFU/g) is larger than or equal to 1.15*10<8>; the bacillus coagulans (CFU/g) is larger than or equal to 2.86*10<8>; the hansenula anomala (CFU/g) is larger than or equal to 2.97*10<8>; and the total amount of organic acids is larger than or equal to 25.8 g/L. The product provided by the invention has an obvious deodorization effect, the NH3 removal rate is greater than or equal to 78.14%, the H2S removal rate is greater than or equal to 71.64%, the odor concentration elimination rate is greater than or equal to 84.95%, and the mosquito and fly expelling rate is greater than or equal to 74.16%.

The invention discloses a compound biological synergist for petrochemical wastewater treatment and a preparation method thereof. The compound biological synergist comprises a complex flora and nutrients, wherein the complex flora is Rhodopseudomonas, Rhodospirillum rubrum, Lactobacillus acidophilus, Pediococcus, Bacillus subtilis, yeast, Acinetobacter, Lampropedia sp. and Thiobacillus denitrificans, and the nutrients are sodium humate, glucose, citric acid and glutamic acid coagulate. The invention relates to the technical field of water treatment. By using the compound biological synergist for petrochemical wastewater treatment and the preparation method thereof, the problems that petrochemical wastewater is susceptible to impact and microorganism poisoning is well solved, By adding high-efficiency bacteria and nutrients, the invention enhances the impact resistance and toxicity resistance of the biochemical system, improves the effluent quality, can improve the load for treating organic pollutants and the stability of microbial structure, improves the ability of the biochemical system to resist toxic and harmful substances, and provides reliable guarantee for further improving the effluent quality.

The invention belongs to the technical field of microbial culture, and specifically relates to a rhodopseudomonas palustris culture medium and an application thereof. The rhodopseudomonas palustris culture medium has a formula composed of citric acid, a beef extract, a yeast extract, peptone, sodium acetate, magnesium sulfate, ferrous sulfate, VB6, calcium chloride, monopotassium phosphate and a composite inorganic salt solution, wherein a solvent is water. According to the invention, through optimization of the formula, the quality of a rhodopseudomonas palustris inoculum is greatly improved,so the rhodopseudomonas palustris inoculum is high in effective viable count, pure in color, free of layering, low in infectious microbe rate, stable in quality and good in preservation effect, and the rhodopseudomonas palustris inoculum cultured by using the culture medium provided by the invention has good effect when being applied to aquaculture.

The invention relates to a photosynthetic bacterium strain and liquid manure rich in photosynthetic bacterium strain and belongs to the technical fields of microbial fermentation, agricultural planting and the like. The photosynthetic bacterium strain is identified as Rhodopseudomonas sp. HL with the preservation number CGMCC No.11874 and can reproduce and grow rapidly by a biogas slurry matrix under an illumination or dark condition. The invention further provides application of the photosynthetic bacterium strain as the microorganism liquid manure. According to the application, the liquid manure is obtained by culturing the Rhodopseudomonas sp. HL in fresh biogas slurry serving as a raw material, and Rhodopseudomonas sp. HL concentration in bacterium liquid is not less than 108CFU/mL. The photosynthetic bacterium strain, the liquid manure rich in the photosynthetic bacterium strain and the application of the photosynthetic bacterium strain have the advantages that biogas slurry use and photosynthetic bacterium culture are coupled, so that the microbial liquid manure with multiple special properties is produced, and a cheap green biogas slurry high-added-value development technology and a cheap green biogas slurry high-added-value product are provided.

The invention discloses a compound microorganism water-purification agent and a preparation method thereof. The water-purification agent is composed of bacillus megaterium, bacillus circulans, rhodopseudomonas palustris, nitrobacter and lactobacillus plantarum. In a culture process, after all strains are cultured separately, mixed culture of the strains is carried out according to the ratio of all strains, so that the finished product is obtained finally. The density of each of five strains in the agent can reach 7-10*10<8>/ml/. By adopting the method provided by the invention, all strains can grow effectively, so that effective microorganism concentration of the compound microorganism water-purification agent is ensured; after the compound microorganism water-purification agent is fed into recycled water receiving water, the effective concentration of microorganisms is high, various microorganism strains play corresponding roles to effectively remove COD, ammonia nitrogen, TN, TP and other pollutants in water originally, particularly, the removal rate for TN is high, and the compound microorganism water-purification agent can change chromaticity and odor of water, and has an obvious water purification effect.

The invention provides biological activated lignite synergistic sulfate reducing bacteria sludge immobilized particles and a preparation method and application thereof. The immobilized particles can be used for reducing, adsorbing and removing various metal cations and anions in acid mine wastewater, and the preparation method of the immobilized particles comprises the following steps: 1) polyvinyl alcohol and sodium alginate are added into water, and swelling, heating and stirring are conducted until bubble-free gel is formed; 2) lignite is added into the gel obtained in the step 1), and uniform stirring and cooling are conducted; 3) rhodopseudomonas sphaeroides liquid and sulfate reducing bacteria sludge are added into the gel mixture obtained in the step 2), the mixture is uniformly stirred, and particles are prepared from the uniformly stirred mixture; 4) the particles obtained in the step 3) are dropwise added into a CaCl2-containing saturated boric acid aqueous solution with the pH value of 5.0-7.0, and crosslinking is carried out under stirring; and then normal saline is used for washing, and moisture on the surface is adsorbed to obtain the biological activated lignite synergistic sulfate reducing bacteria sludge immobilized particles.

The invention discloses a culture medium and a culture method of high-concentration photosynthetic bacteria, wherein the culture method comprises the following steps: implementing fermentation culture of rhodopseudomonas capsulate by virtue of the culture medium provided by the invention, and at the 36th hour and 60th hour of culture, supplementing a nutrition enhancer concentrate for the growth of the rhodopseudomonas capsulate so as to prolong the logarithmic phase of the rhodopseudomonas capsulate; during four-level fermentation of the photosynthetic bacteria, improving illumination intensity by 10-20% every 12h; determining an absorption value , namely OD600nm value, of a ten-time diluted fermentation broth under a 600nm of wavelength by virtue of a visible spectrophotometer; and ending the fermentation until the OD600nm value is not increased anymore, wherein the concentration of the rhodopseudomonas capsulate in the fermentation broth can reach 20x10<9>/mL. The culture medium and the culture method disclosed by the invention have an important guiding significance for the large-scale production of high-concentration photosynthetic bacteria.

The invention discloses a microbial agent for improving soil salinization, which comprises the following components in parts by weight: 85-90 parts of rhodopseudomonas palustris or a culture thereof, 0.1-0.3 part of polyacrylamide, 5-10 parts of polyglutamic acid, 1-3 parts of amino acid powder and 1-2 parts of hydrolyzed polymaleic anhydride. The effective live bacteria count in the rhodopseudomonas palustris or the culture thereof is 2*10<8>-1*10<9>/ml. The microbial agent for improving the soil salinization can effectively improve the quality of the salinized soil, reduce the salinization, reduce the EC value of the soil, promote seed germination and promote plant growth. Tests prove that the microbial agent for improving the soil salinization can reduce the conductivity and pH of the salinized soil, improve the granular structure of the soil, increase the germination rate of pakchoi and effectively improve the salinized soil.

The invention relates to the technical field of microbial culture, and in particular to a compound microbial preparation and a culture method and application thereof. The culture method includes: adopting a mixed culture technology, respectively subjecting Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium bifidum, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae and Candida utilis strains to activation, liquid strain cultivation, composite strain cultivation and finished product cultivation to obtain the compound microbial preparation. The preparation can be used for pig breeding, and has effects of promoting growth, helping digestion, resisting diseases, increasing animal body weight and reducing feed consumption. The methodimproves biomass and physiological metabolic function of microorganisms, makes up for shortcomings of independent culture, uses symbiosis, partial symbiosis, etc. to promote growth of compound microorganisms, and has many obvious advantages compared with a traditional pure culture technology.

The invention discloses biochar suitable for loading photosynthetic bacteria system rhodopseudomonas and a preparation method and application thereof. The preparation method includes the steps that biomass raw materials, ammonium bicarbonate, calcium magnesium phosphate fertilizer and phosphoric acid are mixed, swelling permeation is conducted at the temperature of 40-70 DEG C, and then carbonization is conducted at the temperature of 150-350 DEG C so that the biochar is obtained. The honeycomb structure biochar prepared by the invention is rich in charged functional groups such as hydroxyl, carboxyl, quinonyl and the like, rich in N, O and P elements, rich in pore structures and better in mechanical strength, provides an appropriate living environment for bacilus rhodopseudomonas with thewidth of 0.5-0.9 [mu]m and the length of 1.2-2.0 [mu]m, and the bacilus rhodopseudomonas can be used for repairing black and odorous water, removing organic pollutants in the water, removing nitrogenand phosphorus and restoring the self-purification capacity of the water.

The invention belongs to biological field and relates to a facultative anaerobic marine Rhodopseudomonas sp. strain, active extract from a culture of the strain and a preparation method thereof, and application value of the extract in antibacterial drugs. A marine strain PSB-02 separated from Bohai Sea of China is identified to rhodobacter marinum. Oily matters in three effective parts are obtained from ethyl acetate extract phase and isobutanol extract phase through silica gel thin-layer chromatography and high performance liquid separation, have resistance to pseudomonas fluorescens, pseudomonas aeruginosa, staphylococcus epidermidis and bacilus subtilis, and have a potential use as antibacterial drugs.

The invention discloses Rhodopseudomonas palustris P-3. The preservation number of the Rhodopseudomonas palustris P-3 is CCTCC (China Center For Type Culture Collection) NO: M 2020808. According to the strain, photosynthetic bacteria are separated and purified by adopting a double-layer plate coating method and a streaking method, vibrio parahaemolyticus, vibrio vulnificus and vibrio anguillarum are used as indicator bacteria, and the bacteriostatic action of the marine photosynthetic bacteria strain is determined by adopting an oxford cup method. Naphthalene ethylenediamine hydrochloride spectrophotometry and indophenol blue spectrophotometry are adopted to determine the nitrite nitrogen and ammonia nitrogen degradation effects of different strains, and screening is performed to obtain the product. The strain P-3 has the composite functions of resisting vibrio and efficiently degrading nitrite nitrogen and ammonia nitrogen, has a strong inhibition effect on vibrio anguillarum, has thestrongest effect on vibrio anguillarum, and also has a certain nitrite nitrogen and ammonia nitrogen degradation effect. The strain P-3 is cultured in a culture medium containing 50mg/L ammonia nitrogen and nitrite nitrogen for 4 days, the degradation rates are 89.68% and 94.98% respectively, and the strain P-3 has a wide application prospect.

The invention discloses a vector recombination and construction method, a photosynthetic bacteria rhodopseudomonas palustris fluorescent GFP marker vector construction and electrotransformation method. The recombinant vector includes the photosynthetic bacteria rhodopseudomonas palustris fluorescent GFP marker vector including a PBBR1MCS-2 shuttle vector and a full-length fragment of a green fluorescent protein marker gene GFP, and is fused with a photosynthetic bacteria genome expression promoter PpckA gene and terminator TpckA gene, as well as a widely applied GFP gene, and a full-length fragment sequence of the GFP gene; the full-length fragment of the GFP gene is located between two ECORI and SACI restricted restriction enzyme sites of the PBBR1MCS-2 shuttle vector. The construction method includes the steps: genome DNA extraction, primer designing, PCR amplification, enzyme digestion, connection and other steps. The invention also includes obtained engineering bacteria containingthe recombinant vector, wherein the engineering bacteria are obtained by electrotransformation of the recombinant vector into competent cells. The engineering bacteria have the advantages of high stability, simple technology and environmental friendliness, and can be applied for study of a colonization behavior of photosynthetic bacteria in plants.

The invention discloses an anti-stress microbiological preparation for prawns. The microbiological preparation comprises composite microorganism bacteria, wherein the composite microorganism bacteriacomprise the following strains in percentage by weight of 20-60% of bacillus subtilis, 10-50% of beer microzyme, 3-40% of lactobacillus casei and 10-50% of rhodopseudomonas palustris. The invention further discloses a preparation method of the microbiological preparation. The preparation method comprises the steps of respectively inoculating a corresponding culture medium with four strains, and performing propagation culture to obtain strains; and mixing the strains in a proportion to obtain a composite microorganism bacterium stock solution, then performing inoculating in a total culture medium, and then performing fermentation to prepare the required microbiological preparation. The microbiological preparation disclosed by the invention can be added to any prawn feeds, wherein the microbiological preparation can rapidly remove.OH free radicals in prawns, and can reduce the cortisol and blood sugar concentration in serum, so as to reduce the influence of the stress response of the prawns on organisms, and improve the resistance of the prawns.

The invention provides a microbial soil conditioner for lithified soil, which is prepared from the following strains in percentage by mass: 5-45% of Bacillus megaterium ACCC No.11011, 15-40% of Bacillus subtilis ATCC No.6633, 5-30% of Rhodopseudom onas Palustris ATCCNo.33872, 10-30% of Candida utilis ATCC No.22023 and 20-35% of Azotobacter chroococcum ACCC No.10096.

The invention discloses a water pollution ecological restoration additive and a preparation method thereof. The water pollution ecological restoration additive is prepared from a microbial agent, a microbial carrier and an adsorbent in a mass ratio of 1: (1-5): (3-10), wherein the microbial agent is prepared from bacillus subtilis, rhodopseudomonas palustris, aspergillus niger, EM bacteria, bacillus amyloliquefaciens, frankia and geobacillus stearothermophilus, the microbial carrier is prepared from sodium alginate, chitosan and diatomite and the adsorbent is composed of Fe3O4 nano microspheres, a zeolite molecular sieve, polyacrylate resin and polyacrylamide. The restoration additive prepared by taking various strains and the magnetic adsorbent as main raw materials can be used for rapidly removing excessive pollutants such as ammonia nitrogen, phosphorus, COD (Chemical Oxygen Demand) and heavy metal ions in water, provides an effective way for remediating water body pollution, and has a good market prospect.