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Culture medium and culture method of high-concentration photosynthetic bacteria

A technology of photosynthetic bacteria and culture methods, applied in the direction of bacteria, etc., can solve the problems of lack of nutrients, difficulty in maintaining the growth of bacterial cells, and weakening the ability of light penetration

Inactive Publication Date: 2015-11-25
厦门市科环海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the middle and late stages of the natural fermentation process, due to the lack of nutrients, it is difficult to maintain the further growth of bacterial cells; in addition, as the concentration of bacterial cells increases, the light penetration ability is weakened, which affects the growth of photosynthetic bacteria

Method used

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  • Culture medium and culture method of high-concentration photosynthetic bacteria

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Effect test

Embodiment 1

[0020] A culture medium for high-concentration photosynthetic bacteria, comprising the following components:

[0021] Sodium acetate 5.0g / L, sodium propionate 1.0g / L, yeast extract 4.0g / L, sodium chloride 2.0g / L, dipotassium hydrogen phosphate 0.5g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, sodium bicarbonate 2.0g / L, ammonium sulfate 1.0g / L, auxiliary nutrient solution 2mL, water 998mL, pH7.0~7.5; among them, the auxiliary nutrient solution is: niacinamide 0.005g / L, biological Vitamin 0.001g / L, calcium pantothenate 0.003g / L, vitamin B10.007g / L, vitamin B60.007g / L.

Embodiment 2

[0023] A method for cultivating high-concentration photosynthetic bacteria, comprising the following steps:

[0024] Step 1, strain preparation: use Rhodopseudomonas capsulata as the culture strain;

[0025] Step 2, preparation of fermentation medium: preparing medium according to the above-mentioned components;

[0026] Step 3, primary seed culture: insert a single colony of Rhodopseudomonas capsulata into a sterilized 30mL test tube culture medium, and seal it with a rubber stopper; cultivate it in a light incubator at a constant temperature at a temperature of 30-35°C and light intensity 3000~5000Lx; cultured to dark brown red, the bacterial concentration is 10 9 ~10 10 individual / mL, the culture was terminated, and the first-grade seed solution was obtained;

[0027] Step 4, secondary seed culture: use the primary seed liquid as the seed liquid, insert the inoculation amount of 15%~20% by volume into the sterilized 1000mL triangular flask culture solution, and seal it w...

Embodiment 3

[0032] Sampling was carried out every six hours, and OD was performed on 10-fold dilutions of the fermentation broth by a spectrophotometer 600nm value determination, the result is as figure 1 shown. After the experiment, the OD of the fermentation broth diluted 10 times in the control group 600nm The value is 1.28. while the OD of the experimental group 600nm The value was 2.57, twice that of the control group. Ferment according to the cultivation method of the present invention, the photosynthetic bacteria concentration in the fermented liquid will reach 20 * 10 9 individual / mL.

[0033] In the present invention, at the 36th hour and the 60th hour during the culture process, the nutrient enhancer concentrate for the growth of Rhodopseudomonas capsulata is supplemented to prolong the logarithmic phase of the growth of Rhodopseudomonas capsulatum. During the four-stage fermentation of photosynthetic bacteria, increase the light intensity by 10% to 20% every 12 hours. Ad...

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Abstract

The invention discloses a culture medium and a culture method of high-concentration photosynthetic bacteria, wherein the culture method comprises the following steps: implementing fermentation culture of rhodopseudomonas capsulate by virtue of the culture medium provided by the invention, and at the 36th hour and 60th hour of culture, supplementing a nutrition enhancer concentrate for the growth of the rhodopseudomonas capsulate so as to prolong the logarithmic phase of the rhodopseudomonas capsulate; during four-level fermentation of the photosynthetic bacteria, improving illumination intensity by 10-20% every 12h; determining an absorption value , namely OD600nm value, of a ten-time diluted fermentation broth under a 600nm of wavelength by virtue of a visible spectrophotometer; and ending the fermentation until the OD600nm value is not increased anymore, wherein the concentration of the rhodopseudomonas capsulate in the fermentation broth can reach 20x10<9> / mL. The culture medium and the culture method disclosed by the invention have an important guiding significance for the large-scale production of high-concentration photosynthetic bacteria.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a culture medium of high-concentration photosynthetic bacteria and a culture method thereof. Background technique [0002] Photosynthetic bacteria (PSB), referred to as photosynthetic bacteria, is a class of Gram-negative bacteria that perform anaerobic photosynthesis under anaerobic light conditions or use organic matter as hydrogen donors and carbon sources under aerobic dark conditions. In terms of classification, it belongs to the order Rhodospiraceae, including four families including Rhodospiraceae, Rhodosulfuraceae, Chlorosulfuraceae and Green Filamentaceae. There are a total of 18 genera and about 40 species. Photosynthetic bacteria themselves are rich in nutrients, and the protein content in cell dry matter is as high as 60%. At the same time, they are also rich in physiologically active substances such as natural antioxidant coenzyme Q and carotenoids. Photosynthetic bacte...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 蔡荣淮周勇戴志清
Owner 厦门市科环海洋生物科技有限公司
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