Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Photosynthetic bacterium strain, liquid manure containing same, preparation method of liquid manure and application of liquid manure

A photosynthetic bacteria and liquid technology, which is applied in the field of microbial fermentation and agricultural planting, can solve the problems of destroying the natural components of biogas slurry, the destruction of natural components of biogas slurry, and the high cost of treatment and operation, so as to improve the soil microbial flora, solve the difficulty of digestion, The effect of improving merchandise and added value

Active Publication Date: 2016-04-20
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the high additional development and utilization of biogas slurry has also been reported successively. For example, CN105254367A discloses a "method for preparing special fertilizer for drip irrigation from biogas slurry". This method uses clarified biogas slurry as the base material and adds nutrients and microorganisms Special fertilizer for drip irrigation can be prepared by mixing bacterial agents, but this method requires a large investment in equipment and high processing and operating costs
CN102503660A discloses a "high-efficiency microbial liquid bacterial fertilizer made from biogas slurry and its application". The bacterial fertilizer is based on clarified biogas slurry, microbial functional bacteria, plant growth regulator, and diffusing agent. The microbial liquid fertilizer was prepared by mixing, but a large amount of inorganic chemical components were added to the fertilizer, which destroyed the natural components of the biogas slurry
[0003] To sum up, the high value-added development of biogas slurry mostly stays at the technical level of biogas slurry concentration and biogas slurry mixing. These technologies either have huge investment in equipment and high operating costs; or have problems such as destroying the natural components of biogas slurry.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Photosynthetic bacterium strain, liquid manure containing same, preparation method of liquid manure and application of liquid manure
  • Photosynthetic bacterium strain, liquid manure containing same, preparation method of liquid manure and application of liquid manure
  • Photosynthetic bacterium strain, liquid manure containing same, preparation method of liquid manure and application of liquid manure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) The breeding method of Rhodopseudomonas strain HL, the steps are as follows:

[0035]1) Grind and suspend 10g of the sediment from the fish pond in Xinjie Village, Yongan Town, Shuangliu District, Sichuan, and suspend it in 90mL sterile distilled water, shake evenly, boil for 15min, cool down and then dilute in gradient, and spread it on the screening medium, at 30°C Under culture 72h, obtain bacterium colony, the composition of screening medium is photosynthetic bacteria solid medium (g / L) is: yeast extract 1g, MgCl 2 0.2g, NH 4 Cl1g, K 2 HPO 4 0.5g, NaCl1g, NaHCO 3 1g, add water to 1000mL, adjust pH to 6.8.

[0036] 2) Select 6 bacterial colonies with bright red colonies, vigorous growth, and smooth surface. Under the same culture conditions, these 6 bacterial strains are repeatedly streaked and cultured for more than three times to obtain pure bacterial strains, which are inoculated in the photosynthetic bacteria liquid medium. Cultivate at 30°C for 72 hours...

Embodiment 2

[0042] The percentage by weight of each component of the microbial liquid fertilizer based on the raw material of biogas slurry is: 95% of the bacteria solution rich in Rhodopseudomonas HL cultivated with the biogas slurry as the raw material, and 5% of the glycerin protective agent. Various technical indicators and harmless indicators of finished fertilizers are shown in Table 2 and Table 3 below.

[0043] Table 2 Technical indicators of microbial liquid fertilizer

[0044]

[0045] Table 3 The harmless index of microbial liquid fertilizer

[0046]

Embodiment 3

[0048] The percentage by weight of each component of the microbial liquid fertilizer based on the biogas slurry raw material is: 97% of the bacteria liquid rich in Rhodopseudomonas HL cultivated with the biogas slurry as the raw material, and 3% of the liquid paraffin protective agent. The technical indicators and harmless indicators of the finished fertilizer are as follows in Table 4 and Table 5.

[0049] Table 4 Technical indicators of microbial liquid fertilizer

[0050]

[0051] Table 5 Harmless indicators of microbial liquid fertilizer

[0052]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a photosynthetic bacterium strain and liquid manure rich in photosynthetic bacterium strain and belongs to the technical fields of microbial fermentation, agricultural planting and the like. The photosynthetic bacterium strain is identified as Rhodopseudomonas sp. HL with the preservation number CGMCC No.11874 and can reproduce and grow rapidly by a biogas slurry matrix under an illumination or dark condition. The invention further provides application of the photosynthetic bacterium strain as the microorganism liquid manure. According to the application, the liquid manure is obtained by culturing the Rhodopseudomonas sp. HL in fresh biogas slurry serving as a raw material, and Rhodopseudomonas sp. HL concentration in bacterium liquid is not less than 108CFU / mL. The photosynthetic bacterium strain, the liquid manure rich in the photosynthetic bacterium strain and the application of the photosynthetic bacterium strain have the advantages that biogas slurry use and photosynthetic bacterium culture are coupled, so that the microbial liquid manure with multiple special properties is produced, and a cheap green biogas slurry high-added-value development technology and a cheap green biogas slurry high-added-value product are provided.

Description

technical field [0001] The invention relates to a microbial strain and a liquid fertilizer rich in the strain, in particular to a microbial liquid fertilizer based on biogas slurry raw materials, a preparation method and application thereof, and belongs to the technical fields of microbial fermentation and agricultural planting. Background technique [0002] With the development of my country's livestock and poultry breeding industry, the annual production of livestock and poultry manure has reached 2.5 billion tons. At the same time, my country is a large agricultural country, and agricultural production produces more than 700 million tons of crop straw every year. Anaerobic fermentation technology, represented by biogas engineering, is an environmentally friendly technology that can absorb organic waste such as livestock and poultry manure, crop stalks, and household garbage, and alleviate energy shortages, and has been increasingly widely used. According to statistics, as...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C05F15/00C05F11/08C12R1/01
CPCC05F7/00C12N1/205C12R2001/01C05F11/08
Inventor 闫志英袁月祥许力山廖银章李志东李东刘晓风
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products