Photosynthetic bacterium strain, liquid manure containing same, preparation method of liquid manure and application of liquid manure
A photosynthetic bacteria and liquid technology, which is applied in the field of microbial fermentation and agricultural planting, can solve the problems of destroying the natural components of biogas slurry, the destruction of natural components of biogas slurry, and the high cost of treatment and operation, so as to improve the soil microbial flora, solve the difficulty of digestion, The effect of improving merchandise and added value
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Embodiment 1
[0034] (1) The breeding method of Rhodopseudomonas strain HL, the steps are as follows:
[0035]1) Grind and suspend 10g of the sediment from the fish pond in Xinjie Village, Yongan Town, Shuangliu District, Sichuan, and suspend it in 90mL sterile distilled water, shake evenly, boil for 15min, cool down and then dilute in gradient, and spread it on the screening medium, at 30°C Under culture 72h, obtain bacterium colony, the composition of screening medium is photosynthetic bacteria solid medium (g / L) is: yeast extract 1g, MgCl 2 0.2g, NH 4 Cl1g, K 2 HPO 4 0.5g, NaCl1g, NaHCO 3 1g, add water to 1000mL, adjust pH to 6.8.
[0036] 2) Select 6 bacterial colonies with bright red colonies, vigorous growth, and smooth surface. Under the same culture conditions, these 6 bacterial strains are repeatedly streaked and cultured for more than three times to obtain pure bacterial strains, which are inoculated in the photosynthetic bacteria liquid medium. Cultivate at 30°C for 72 hours...
Embodiment 2
[0042] The percentage by weight of each component of the microbial liquid fertilizer based on the raw material of biogas slurry is: 95% of the bacteria solution rich in Rhodopseudomonas HL cultivated with the biogas slurry as the raw material, and 5% of the glycerin protective agent. Various technical indicators and harmless indicators of finished fertilizers are shown in Table 2 and Table 3 below.
[0043] Table 2 Technical indicators of microbial liquid fertilizer
[0044]
[0045] Table 3 The harmless index of microbial liquid fertilizer
[0046]
Embodiment 3
[0048] The percentage by weight of each component of the microbial liquid fertilizer based on the biogas slurry raw material is: 97% of the bacteria liquid rich in Rhodopseudomonas HL cultivated with the biogas slurry as the raw material, and 3% of the liquid paraffin protective agent. The technical indicators and harmless indicators of the finished fertilizer are as follows in Table 4 and Table 5.
[0049] Table 4 Technical indicators of microbial liquid fertilizer
[0050]
[0051] Table 5 Harmless indicators of microbial liquid fertilizer
[0052]
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