Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rhodopseudomonas palustris LY-6 microbial agent and preparation method thereof and application thereof in prevention and treatment of southern blight of tomatoes

The technology of Rhodopseudomonas swamp and Rhodopseudomonas is applied in the directions of application, fungicides, chemicals for biological control, etc. The production process is simple, the cost is low, and the effect of drug resistance is not easy

Inactive Publication Date: 2018-11-27
HUNAN PLANT PROTECTION INST
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned method for preventing and treating tomato white silk disease has a long process, slow effect, inconvenient implementation, and is easy to cause pollution to the environment. Long-term use of pesticides will reduce the control effect and produce drug resistance.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A Rhodopseudomonas palustris LY-6 inoculum, which is prepared from Rhodopseudomonas palustris strains through activation, seed culture, and production culture.

[0021] A preparation method of Rhodopseudomonas palustris LY-6 inoculum includes the following steps:

[0022] Step 1: Activation: The preserved strain of Rhodopseudomonas palustris is cultivated according to the double-layer plate culture method until a single red colony appears. The medium used is a photosynthetic bacteria solid medium, and the formula is: (NH4) 2 SO4 1.6g / L, NaCO 3 0.8g / L, yeast extract 1.2g / L, K 2 HPO4 0.2g / L, MgSO 4 0.3g / L, agar powder 18g / L, pH=7.0;

[0023] Step 2: Seed culture: Put the red single colony cultured in step 1 into the serum bottle, and use the Rhodopseudomonas palustris liquid medium for seed culture to the logarithmic growth phase to obtain the bacterial liquid. The temperature of seed culture is 30℃, light conditions are 2500Lx, pH is 7.0;

[0024] Step 3: Production and cultiv...

Embodiment 2

[0035] A Rhodopseudomonas palustris inocula, which is prepared by activation, seed culture and production culture of Rhodopseudomonas palustris strain.

[0036] A preparation method of Rhodopseudomonas palustris LY-6 inoculum includes the following steps:

[0037] Step 1: Activation: The preserved strain of Rhodopseudomonas palustris is cultivated according to the double-layer plate culture method until a single red colony appears. The medium used is a photosynthetic bacteria solid medium, and the formula is: (NH4) 2 SO4 1.7g / L, NaCO 3 0.9g / L, yeast extract 1.3g / L, K 2 HPO4 0.3g / L, MgSO 4 0.5g / L, agar powder 19g / L, pH=7.0;

[0038] Step 2: Seed culture: Put the red single colony cultured in step 1 into the serum bottle, and use the Rhodopseudomonas palustris liquid medium for seed culture to the logarithmic growth phase to obtain the bacterial liquid. The temperature of seed culture is 31℃, light condition is 3200Lx, pH is 7.0;

[0039] Step 3: Production and cultivation: Inoculate ...

Embodiment 3

[0050] A Rhodopseudomonas palustris LY-6 inoculum, which is prepared from Rhodopseudomonas palustris strains through activation, seed culture, and production culture.

[0051] A preparation method of Rhodopseudomonas palustris LY-6 inoculum includes the following steps:

[0052] Step 1: Activation: The preserved strain of Rhodopseudomonas palustris is cultivated according to the double-layer plate culture method until a single red colony appears. The medium used is a photosynthetic bacteria solid medium, and the formula is: (NH4) 2 SO4 1.8g / L, NaCO 3 1.0g / L, yeast extract 1.5g / L, K 2 HPO4 0.4g / L, MgSO 4 0.6g / L, agar powder 20g / L, pH=7.0;

[0053] Step 2: Seed culture: Put the red single colony cultured in step 1 into the serum bottle, and use the Rhodopseudomonas palustris liquid medium for seed culture to the logarithmic growth phase to obtain the bacterial liquid. The temperature of seed culture is 32℃, the light condition is 4000Lx, the pH is 7.0;

[0054] Step 3: Production and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a phodopseudomonas palustris LY-6 microbial inoculum, and a preparation method thereof and application thereof in prevention and treatment of southern blight of tomatoes. A phodopseudomonas palustris bacterial strain is phodopseudomonas palustris; and the phodopseudomonas palustris microbial inoculum is prepared through activation of the phodopseudomonas palustris bacterialstrain, seed culture and production culture. The phodopseudomonas palustris is simple in production process, short in culture time and low in cost; and a biocontrol microbial inoculum and biocontrol fermentation liquor which are used for the prevention and control of the southern blight of tomatoes can be prepared from the strain by a simple, rapid and low-cost operation method. The biocontrol microbial inoculum and the biocontrol fermentation liquor prepared from the phodopseudomonas palustris are environmentally-friendly, non-toxic to humans and animals, harmless to crops, simple and convenient to apply, and not liable to cause drug resistance to plant diseases and insect pests.

Description

Technical field [0001] The invention relates to the technical field of biological pesticides, in particular to the preparation of a Rhodopseudomonas palustris LY-6 inoculum and its application to the prevention and treatment of tomato white sclerosis. Background technique [0002] Microorganisms are widely present in nature. They have become a research hotspot because of their non-toxic, harmless, non-polluting, high-efficiency and resistance to pesticides in the process of preventing and controlling plant diseases and insect pests. Photosynthetic bacteria (Photosynthetic Bacteria, PBS) is a general term for a type of bacteria that can perform photosynthesis without releasing oxygen under anaerobic conditions, and is the first prokaryotic organism with a primitive light energy synthesis system to appear on the earth. The known photosynthetic bacteria share three families: Chlorobiaceae, Chromatiaceae, and Rhodospirillaceae. Rhodopseudanonas palustris (Rhodopseudanonas palustris)...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/38
CPCA01N63/00C12N1/20
Inventor 唐雯苏品程菊娥刘勇张德咏谭新球翟忠英陈丽洁孔小婷杜晓华
Owner HUNAN PLANT PROTECTION INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com