Compound microorganism water-purification agent, preparation method and application
A technology for compound microorganisms and water purification, applied in the field of compound microorganism water purification preparations, can solve the problems of poor impact resistance, unsatisfactory treatment effect, poor removal effect of total nitrogen and ammonia nitrogen, etc., and achieves high removal rate and significant water purification effect. , to ensure the effect of effective microbial concentration
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[0028] The invention also discloses a preparation method of a composite microbial water purification preparation, comprising the following steps:
[0029] Step 1, inoculate Bacillus megaterium, Bacillus circulans, Rhodopseudomonas palustris, Nitrobacter and Lactobacillus plantarum into the culture medium and culture them separately, so that the density of each strain reaches 7-10×10 8 / ml;
[0030] In step 2, the strains cultivated in step 1 are mixed and inoculated in the fermentation broth in proportion to prepare a composite microbial water purification preparation; wherein, the strains are preferably (1) 1.5: 2.5: 2: 2.5 in weight ratio: 1.5; (2) 1.5: 2.2: 1.9: 2.3: 1.4; (3) 1.5: 2.6: 2.1: 2.6: 1.6 are mixed.
[0031] As a preferred embodiment, the preparation method of each strain culture solution in step 1 comprises the following steps respectively:
[0032] (a) After the Bacillus megaterium culture solution is fully mixed, the pH value of the culture solution is adjus...
preparation example
[0054] Step 1, inoculate Bacillus megaterium, Bacillus circulans, Rhodopseudomonas palustris, Nitrobacter and Lactobacillus plantarum into the culture medium and culture them separately, so that the density of each strain reaches 7-10×10 8 / ml;
[0055] In step 2, the strains cultivated in step 1 are mixed and inoculated in the fermentation broth in proportion to prepare a composite microbial water purification preparation, wherein the weight ratio of the strains is 1.5:2.5:2:2.5:1.5.
[0056] Wherein, in the step 1, the preparation method of each bacterial strain culture solution is as follows respectively:
[0057] (a) After the Bacillus megaterium culture solution is fully mixed, the pH value of the culture solution is adjusted to 7.2-7.4, and it is sterilized at 121°C for 20 minutes; the Bacillus megaterium is inoculated in the culture solution, the shaker is 150rpm, 25-30 Cultivation at ℃; wherein, the composition of the Bacillus megaterium culture solution is, for examp...
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