Active extract of facultative anaerobic red marine pseudomonas and production method and use thereof
A Rhodopseudomonas, facultative anaerobic technology, applied in the field of facultative anaerobic marine Rhodopseudomonas
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0035] Embodiment 1. liquid culture medium is:
[0036] Peptone 2.5g;
[0037] Yeast paste 2.5g;
[0038] Artificial seawater (or old seawater) 1000ml;
[0039] Adjust the pH to 6.8-7.0.
[0040] Artificial Seawater Formula:
[0041] NaCl 25.0g;
[0042] Na 2 SO 4 4.0g;
[0043] KCl 0.7g;
[0044] NaHCO 3 0.20g;
[0045] KBr 0.10g;
[0046] h 3 BO 3 0.03g;
[0047] NaF 0.003g;
[0048] 1.0mol / L MgCl 2 Solution 53mL;
[0049] 1.0mol / l CaCl 2 Solution 10mL;
[0050] 0.1mol / L SrO 2 Solution 0.90ml;
[0051] Distilled water 1000ml, adjust the pH to 6.8-7.0.
[0052] A. Seed culture: fill the liquid medium into the serum bottle, leave a 2cm high gap in the bottle, tighten the serum bottle ...
Embodiment 2
[0056] Embodiment 2. liquid culture medium is:
[0057] NaCl 30.4g;
[0058] Yeast paste 1.0g;
[0059] Sodium succinate 1.0g;
[0060] K H 2 PO 4 0.5g;
[0061] MgSO 4 ·7H 2 O 3.0g;
[0062] NH 4 Cl 0.4g;
[0063] CaCl 2 2H 2 O 0.05g;
[0064] Ferric citrate (0.1%) 5.0mL;
[0065] SL~6 1.0mL;
[0066] Absolute ethanol 0.5mL;
[0067] Adjust the pH to 6.8±0.2.
[0068] Among them, SL~6 is a trace element solution, and the formula is:
[0069] h 3 BO 3 0.3g;
[0070] CoCl 2 ·6H 2 O 0.2g;
[0071] ZnSO 4 ·7H 2 O 0.1g;
[0072] MnCl 2 4H 2 O 0.03g;
[0073] NaMoO 4 2H 2 O 0.03g;
[0074] NiCl 2 ·6H 2 O 0.02g;
[0075] CuCl 2 2H 2 O 0.01g;
[0076] Distilled wa...
Embodiment 3
[0082] Example 3. Active component activity assay results
[0083] The agar dilution method (National Committee for Clinical Laboratory Standards.2000.Approved standard: M2~A7, 7th ed.NCCLS, Wayne, Pa.) was used to determine the effect on Pseudomonas fluorescens, Pseudomonas aeruginosa, Staphylococcus epidermidis and subtilis The MIC value of Bacillus confirms that the ethyl acetate extract phase and n-butanol extract phase of the culture solution have antibacterial activity: the MIC value of the two active components prepared in the ethyl acetate phase against Pseudomonas fluorescens is 0.80 μg / mL; the MIC value of anti-Pseudomonas aeruginosa is 0.74μg / mL; the MIC value of the active ingredient prepared in n-butanol phase against Pseudomonas fluorescens is 1.25μg / mL; the anti-Pseudomonas aeruginosa The MIC value of bacteria was 0.61 μg / mL; the MIC value of anti-Staphylococcus epidermidis was 0.86 μg / mL; the MIC value of anti-Bacillus subtilis was 0.78 μg / mL.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com