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Active extract of facultative anaerobic red marine pseudomonas and production method and use thereof

A Rhodopseudomonas, facultative anaerobic technology, applied in the field of facultative anaerobic marine Rhodopseudomonas

Inactive Publication Date: 2009-06-17
DALIAN JIAOTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the isolation and extraction of pharmacologically active secondary metabolites from facultative marine photosynthetic bacteria

Method used

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  • Active extract of facultative anaerobic red marine pseudomonas and production method and use thereof
  • Active extract of facultative anaerobic red marine pseudomonas and production method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1. liquid culture medium is:

[0036] Peptone 2.5g;

[0037] Yeast paste 2.5g;

[0038] Artificial seawater (or old seawater) 1000ml;

[0039] Adjust the pH to 6.8-7.0.

[0040] Artificial Seawater Formula:

[0041] NaCl 25.0g;

[0042] Na 2 SO 4 4.0g;

[0043] KCl 0.7g;

[0044] NaHCO 3 0.20g;

[0045] KBr 0.10g;

[0046] h 3 BO 3 0.03g;

[0047] NaF 0.003g;

[0048] 1.0mol / L MgCl 2 Solution 53mL;

[0049] 1.0mol / l CaCl 2 Solution 10mL;

[0050] 0.1mol / L SrO 2 Solution 0.90ml;

[0051] Distilled water 1000ml, adjust the pH to 6.8-7.0.

[0052] A. Seed culture: fill the liquid medium into the serum bottle, leave a 2cm high gap in the bottle, tighten the serum bottle ...

Embodiment 2

[0056] Embodiment 2. liquid culture medium is:

[0057] NaCl 30.4g;

[0058] Yeast paste 1.0g;

[0059] Sodium succinate 1.0g;

[0060] K H 2 PO 4 0.5g;

[0061] MgSO 4 ·7H 2 O 3.0g;

[0062] NH 4 Cl 0.4g;

[0063] CaCl 2 2H 2 O 0.05g;

[0064] Ferric citrate (0.1%) 5.0mL;

[0065] SL~6 1.0mL;

[0066] Absolute ethanol 0.5mL;

[0067] Adjust the pH to 6.8±0.2.

[0068] Among them, SL~6 is a trace element solution, and the formula is:

[0069] h 3 BO 3 0.3g;

[0070] CoCl 2 ·6H 2 O 0.2g;

[0071] ZnSO 4 ·7H 2 O 0.1g;

[0072] MnCl 2 4H 2 O 0.03g;

[0073] NaMoO 4 2H 2 O 0.03g;

[0074] NiCl 2 ·6H 2 O 0.02g;

[0075] CuCl 2 2H 2 O 0.01g;

[0076] Distilled wa...

Embodiment 3

[0082] Example 3. Active component activity assay results

[0083] The agar dilution method (National Committee for Clinical Laboratory Standards.2000.Approved standard: M2~A7, 7th ed.NCCLS, Wayne, Pa.) was used to determine the effect on Pseudomonas fluorescens, Pseudomonas aeruginosa, Staphylococcus epidermidis and subtilis The MIC value of Bacillus confirms that the ethyl acetate extract phase and n-butanol extract phase of the culture solution have antibacterial activity: the MIC value of the two active components prepared in the ethyl acetate phase against Pseudomonas fluorescens is 0.80 μg / mL; the MIC value of anti-Pseudomonas aeruginosa is 0.74μg / mL; the MIC value of the active ingredient prepared in n-butanol phase against Pseudomonas fluorescens is 1.25μg / mL; the anti-Pseudomonas aeruginosa The MIC value of bacteria was 0.61 μg / mL; the MIC value of anti-Staphylococcus epidermidis was 0.86 μg / mL; the MIC value of anti-Bacillus subtilis was 0.78 μg / mL.

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Abstract

The invention belongs to biological field and relates to a facultative anaerobic marine Rhodopseudomonas sp. strain, active extract from a culture of the strain and a preparation method thereof, and application value of the extract in antibacterial drugs. A marine strain PSB-02 separated from Bohai Sea of China is identified to rhodobacter marinum. Oily matters in three effective parts are obtained from ethyl acetate extract phase and isobutanol extract phase through silica gel thin-layer chromatography and high performance liquid separation, have resistance to pseudomonas fluorescens, pseudomonas aeruginosa, staphylococcus epidermidis and bacilus subtilis, and have a potential use as antibacterial drugs.

Description

technical field [0001] The invention relates to a strain of facultative anaerobic marine rhodopseudomonas, in particular to the active extract of the marine strain culture, its preparation method and the use of the extract in antibacterial drugs. Background technique [0002] The development of new drugs derived from marine microorganisms is a worldwide research hotspot in the 21st century. It has attracted widespread attention due to its characteristics of new strain sources, production of new structural active substances, overcoming traditional antibiotic resistance, and realizing resource sustainability. . At present, many novel antibacterial antibiotics, antifungal antibiotics, antiviral antibiotics, antitumor antibiotics, and some anti-inflammatory and analgesic active substances, enzyme inhibitors, etc. have been isolated from various marine microorganisms. There is a class of facultative anaerobic microorganisms-photosynthetic bacteria in the ocean. These microorgani...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P31/04C12N1/20
Inventor 穆军顾晓洁张翼赵友宝朱秀华董学伟
Owner DALIAN JIAOTONG UNIVERSITY
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