Composite microbial deodorizer and preparation method thereof

A technology of microorganisms and deodorants, which is applied in the field of compound microbial deodorants and its preparation, and can solve problems such as single processing function, inconspicuous deodorizing effect, and incomplete strains.

Active Publication Date: 2020-08-07
巴彦淖尔市云天下电子商务股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in the existing biological deodorization method, such as incomplete strains, low number of viable bacteria, inconspicuous d

Method used

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  • Composite microbial deodorizer and preparation method thereof
  • Composite microbial deodorizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] 1. Perform liquid fermentation on Thiobacillus ferrooxidans, Rhizobium sludge, Bacillus amyloliquefaciens, Rhodopseudomonas palustris, Pseudomonas stutzeri, Bacillus coagulans, and Hansenula anomalies respectively according to the following method :

[0076] (1) Thiobacillus ferrooxidans bacterial liquid is prepared as follows:

[0077] A. Seed medium: ammonium sulfate 6g, yeast extract 5g, glucose 5g, K 2 HPO 4 2g, KH 2 PO 4 3.0g, MgSO 4 ·7H 2 O 0.6g, MnSO 4 ·H 2 O 0.002g, distilled water 1000mL, stirred until completely dissolved, pH 6.8, sterilized at 121°C for 30min.

[0078] B. Liquid fermentation medium: ammonium sulfate 6kg, yeast extract powder 5kg, glucose 5kg, K 2 HPO 4 2kg, KH 2 PO 4 2.5kg, MgSO 4 ·7H 2 O 0.6kg, MnSO 4 ·H 2 O 0.002kg, 1000L of distilled water, stirred until completely dissolved, pH 6.8, sterilized at 121°C for 30min.

[0079] C. Culture conditions:

[0080] Seed culture conditions: 3000ml of seed culture medium in a 5000m...

Embodiment 2

[0120] 1. Perform liquid fermentation on Thiobacillus ferrooxidans, Rhizobium sludge, Bacillus amyloliquefaciens, Rhodopseudomonas palustris, Pseudomonas stutzeri, Bacillus coagulans, and Hansenula anomalies respectively according to the following method :

[0121] (1) Thiobacillus ferrooxidans bacterial liquid is prepared as follows:

[0122] A. Seed medium: ammonium sulfate 3g, yeast extract 2g, glucose 2g, K 2 HPO 4 1g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.3g, MnSO 4 ·H 2 O 0.001g, distilled water 1000mL, stirred until completely dissolved, pH 6.8, sterilized at 121°C for 30min.

[0123] B. Liquid fermentation medium: ammonium sulfate 3kg, yeast extract powder 2kg, glucose 2kg, K 2 HPO 4 1kg, KH 2 PO 4 1kg, MgSO 4 ·7H 2 O 0.3kg, MnSO 4 ·H 2 O 0.001kg, distilled water 1000L, stirred until completely dissolved, pH 6.8, sterilized at 121°C for 30min.

[0124] C. Culture conditions:

[0125] Seed culture conditions: 2500ml of seed culture medium in a 5000ml Erlen...

Embodiment 3

[0165] 1. Perform liquid fermentation on Thiobacillus ferrooxidans, Rhizobium sludge, Bacillus amyloliquefaciens, Rhodopseudomonas palustris, Pseudomonas stutzeri, Bacillus coagulans, and Hansenula anomalies respectively according to the following method :

[0166] (1) Thiobacillus ferrooxidans bacterial liquid is prepared as follows:

[0167] A. Seed medium: ammonium sulfate 5g, yeast extract 3g, glucose 3g, K 2 HPO 4 1.5g, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 0.5g, MnSO 4 ·H 2 O 0.001g, distilled water 1000mL, stirred until completely dissolved, pH 6.8, sterilized at 121°C for 30min.

[0168] B. Liquid fermentation medium: ammonium sulfate 5kg, yeast extract powder 3kg, glucose 3kg, K 2 HPO 4 1.5kg, KH 2 PO 4 2kg, MgSO 4 ·7H 2 O 0.5kg, MnSO 4 ·H 2 O 0.001kg, distilled water 1000L, stirred until completely dissolved, pH 6.8, sterilized at 121°C for 30min.

[0169] C. Culture conditions:

[0170] Seed culture conditions: 2500ml of seed culture medium in a 5000ml E...

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Abstract

The invention discloses a composite microbial deodorizer and a preparation method thereof. According to the invention, thiobacillus ferrooxidans, rhizobium sludge, bacillus amyloliquefaciens, rhodopseudomonas palustris, pseudomonas stutzeri, bacillus coagulans and hansenula polymorpha are selected for use; liquid fermentation is performed respectively, and then compounding is performed in proportion; then linalool is added in proportion and the substances are fully mixed to prepare a composite microbial deodorizer capable of effectively expelling flies and insects, inhibiting growth and propagation of harmful flora and reducing and removing foul gas in the environment. The thiobacillus ferrooxidans (CFU/g) in the product is greater than or equal to 2.95*10<8>; the rhizobium sludge (CFU/g)is greater than or equal to 1.05*10<8>; the bacillus amyloliquefaciens (CFU/g) is greater than or equal to 1.73*10<8>; the rhodopseudomonas palustris (CFU/g) is larger than or equal to 2.55*10<8>; thepseudomonas stutzeri (CFU/g) is larger than or equal to 1.15*10<8>; the bacillus coagulans (CFU/g) is larger than or equal to 2.86*10<8>; the hansenula anomala (CFU/g) is larger than or equal to 2.97*10<8>; and the total amount of organic acids is larger than or equal to 25.8 g/L. The product provided by the invention has an obvious deodorization effect, the NH3 removal rate is greater than or equal to 78.14%, the H2S removal rate is greater than or equal to 71.64%, the odor concentration elimination rate is greater than or equal to 84.95%, and the mosquito and fly expelling rate is greater than or equal to 74.16%.

Description

Technical field: [0001] The invention relates to the technical field of environmental bioengineering, in particular to a compound microbial deodorant and a preparation method thereof. Background technique: [0002] With the rapid development of social economy and the continuous acceleration of urbanization, the problem of indoor and outdoor environmental pollution has become increasingly prominent. As one of the environmental hazards, stench and bacteria breeding in the environment directly affect people's quality of life and even endanger people's health, which has attracted more and more people's attention. [0003] At present, there are more than 4,000 kinds of odorous substances perceived by human sense of smell, which can be divided into domestic sources and industrial sources according to the source of production. The source of life refers to the stench produced in daily life, such as household toilets, public toilets, sewage treatment plants, garbage transfer station...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16B01D53/84B01D53/52B01D53/58B01D53/54B01D53/48B01D53/46C12R1/41C12R1/07C12R1/38C12R1/78C12R1/01
CPCC12N1/20C12N1/16B01D53/84B01D53/58B01D53/52B01D53/485B01D53/54B01D53/46B01D2251/95B01D2257/306B01D2257/55B01D2257/90C12R2001/07C12N1/205C12R2001/38C12R2001/41C12R2001/78C12N1/165C12R2001/01Y02A50/20
Inventor 岳彦王普申
Owner 巴彦淖尔市云天下电子商务股份有限公司
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