Composite microbial preparation for promoting growth of rape and preparation method thereof
A technology of composite microorganisms and microbial carriers, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as single function, nitrogen fixation is easily restricted by many conditions, and no symbiotic tissue structure is formed. The effect of improving soil structure, improving crop stress resistance, inhibiting growth and reproduction
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Embodiment 1
[0026] A composite microbial preparation for promoting the growth of rapeseed, consisting of 20 parts by weight of bacterial culture and 80 parts by weight of microbial carrier; wherein, the bacterial culture is composed of the following raw materials in parts by weight: 2 parts of Bacillus subtilis , 2 parts of Bacillus megaterium, 5 parts of Rhodopseudomonas palustris and 1 part of Rhizobium, all of the above strains can be purchased at the China Agricultural Microorganism Culture Collection and Management Center; the microbial carrier is plant ash.
[0027] The specific steps of the preparation method of the above-mentioned composite microbial preparation for promoting the growth of rapeseed are:
[0028] Step 1: Mix the first liquid medium with Tween-80 at a weight ratio of 100:0.3 after being sterilized at 115°C for 15 minutes, and mix the mixture of Bacillus subtilis, Bacillus megaterium and Rhodopseudomonas palustris by weight Inoculate in the above-mentioned first liqu...
Embodiment 2
[0034] A composite microbial preparation for promoting the growth of rapeseed, consisting of 40 parts by weight of bacterial culture and 60 parts by weight of microbial carrier; wherein, the bacterial culture is composed of the following raw materials in parts by weight: 8 parts of Bacillus subtilis , 8 parts of Bacillus megaterium, 10 parts of Rhodopseudomonas palustris and 4 parts of Rhizobium, all of the above strains can be purchased at the China Agricultural Microorganism Culture Collection and Management Center; the microbial carrier is bentonite.
[0035] The specific steps of the preparation method of the above-mentioned composite microbial preparation for promoting the growth of rapeseed are:
[0036] Step 1: Mix the first liquid culture medium with Tween-80 at a weight ratio of 100:0.5 after being sterilized at 130°C for 30 minutes, and mix the mixture of Bacillus subtilis, Bacillus megaterium and Rhodopseudomonas palustris by weight Inoculate in the above-mentioned ...
Embodiment 3
[0042] A composite microbial preparation for promoting the growth of rapeseed, consisting of 40 parts by weight of bacterial culture and 60 parts by weight of microbial carrier; wherein, the bacterial culture is composed of the following raw materials in parts by weight: 6 parts of Bacillus subtilis , 6 parts of Bacillus megaterium, 6 parts of Rhodopseudomonas palustris and 3 parts of Rhizobium, all of the above strains can be purchased at the China Agricultural Microorganism Culture Collection and Management Center; the microbial carrier is 2:8 by weight A mixture of plant ash and bentonite.
[0043] The specific steps of the preparation method of the above-mentioned composite microbial preparation for promoting the growth of rapeseed are:
[0044] The first step: mix the first liquid medium with Tween-80 at a weight ratio of 100:0.3 after being sterilized at 125°C for 20 minutes, and mix the mixture of Bacillus subtilis, Bacillus megaterium and Rhodopseudomonas palustris by ...
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