Composite microbial preparation for promoting growth of rape and preparation method thereof

A technology of composite microorganisms and microbial carriers, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as single function, nitrogen fixation is easily restricted by many conditions, and no symbiotic tissue structure is formed. The effect of improving soil structure, improving crop stress resistance, inhibiting growth and reproduction

Inactive Publication Date: 2010-03-17
SHANGHAI CHUANGBO SOIL REMEDIATION ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing photosynthetic microbial fertilizers are generally a single strain, and the activity of the bacteria is generally 10 6 -10 8 Cfu / gram, the function is relatively single, crops and microorganisms are only loosely combined, and there is no symbiotic organizational structure between them, so the nitrogen fixation activity is easily restricted by many conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A composite microbial preparation for promoting the growth of rapeseed, consisting of 20 parts by weight of bacterial culture and 80 parts by weight of microbial carrier; wherein, the bacterial culture is composed of the following raw materials in parts by weight: 2 parts of Bacillus subtilis , 2 parts of Bacillus megaterium, 5 parts of Rhodopseudomonas palustris and 1 part of Rhizobium, all of the above strains can be purchased at the China Agricultural Microorganism Culture Collection and Management Center; the microbial carrier is plant ash.

[0027] The specific steps of the preparation method of the above-mentioned composite microbial preparation for promoting the growth of rapeseed are:

[0028] Step 1: Mix the first liquid medium with Tween-80 at a weight ratio of 100:0.3 after being sterilized at 115°C for 15 minutes, and mix the mixture of Bacillus subtilis, Bacillus megaterium and Rhodopseudomonas palustris by weight Inoculate in the above-mentioned first liqu...

Embodiment 2

[0034] A composite microbial preparation for promoting the growth of rapeseed, consisting of 40 parts by weight of bacterial culture and 60 parts by weight of microbial carrier; wherein, the bacterial culture is composed of the following raw materials in parts by weight: 8 parts of Bacillus subtilis , 8 parts of Bacillus megaterium, 10 parts of Rhodopseudomonas palustris and 4 parts of Rhizobium, all of the above strains can be purchased at the China Agricultural Microorganism Culture Collection and Management Center; the microbial carrier is bentonite.

[0035] The specific steps of the preparation method of the above-mentioned composite microbial preparation for promoting the growth of rapeseed are:

[0036] Step 1: Mix the first liquid culture medium with Tween-80 at a weight ratio of 100:0.5 after being sterilized at 130°C for 30 minutes, and mix the mixture of Bacillus subtilis, Bacillus megaterium and Rhodopseudomonas palustris by weight Inoculate in the above-mentioned ...

Embodiment 3

[0042] A composite microbial preparation for promoting the growth of rapeseed, consisting of 40 parts by weight of bacterial culture and 60 parts by weight of microbial carrier; wherein, the bacterial culture is composed of the following raw materials in parts by weight: 6 parts of Bacillus subtilis , 6 parts of Bacillus megaterium, 6 parts of Rhodopseudomonas palustris and 3 parts of Rhizobium, all of the above strains can be purchased at the China Agricultural Microorganism Culture Collection and Management Center; the microbial carrier is 2:8 by weight A mixture of plant ash and bentonite.

[0043] The specific steps of the preparation method of the above-mentioned composite microbial preparation for promoting the growth of rapeseed are:

[0044] The first step: mix the first liquid medium with Tween-80 at a weight ratio of 100:0.3 after being sterilized at 125°C for 20 minutes, and mix the mixture of Bacillus subtilis, Bacillus megaterium and Rhodopseudomonas palustris by ...

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PUM

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Abstract

The invention relates to a composite microbial preparation for promoting the growth of rape and a preparation method thereof. The composite microbial preparation for promoting the growth of the rape is characterized by comprising 20-40 parts by weight of strain culturing substance and 60-80 parts by weight of microbial carrier, wherein the strain culturing substance comprises the following raw materials in parts by weight: 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium, 5-10 parts of rhodopseudomonas palustris and 1-4 parts of azotobacter chroococcum. The preparation method of the composite microbial preparation for promoting the growth of the rape comprises the following steps: culturing 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium and the rhodopseudomonas palustris together, culturing the azotobacter chroococcum independently, mixing and fermenting. The invention has the advantages of promoting the growth and the propagation of beneficial microorganisms, increasing the absorption of the root system of the rape to nitrogen, phosphorus, potassium and other organic substances and promoting the normal development of the root system, thus promoting the growth of the rape and reducing the applied chemical fertilizer.

Description

technical field [0001] The invention relates to a compound microbial preparation for promoting the growth of rapeseed and a preparation method thereof, which is applied to plant roots in soil to fix nitrogen in the air as organic nitrogen or supplement nitrogen in soil by salt, and belongs to the field of organic fertilizers. Background technique [0002] Rapeseed has an annual planting area of ​​about 8 million hectares in my country, and its seeds have an oil content of 30%-50%. It is an important oil crop in my country. For a long time, my country's rapeseed planting area and total output have ranked first in the world, accounting for about 30% of the world's rapeseed planting area and total output. After my country's accession to the WTO, various agricultural product markets in our country are faced with a long-term protected position and lack of strong market competitiveness. In addition, with the further development of the international market, whether my country's ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C05G3/00C05G3/02C05G3/04C12N1/20C12R1/125C12R1/11C12R1/01C12R1/41C05G3/60C05G3/80
Inventor 江瀚张玉辉
Owner SHANGHAI CHUANGBO SOIL REMEDIATION ENG
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