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Method for preparing freeze-dried microbial agent of kluyveromyces marxianus and application of freeze-dried microbial agent

A technology for yeast and dry inoculum is applied in the field of preparation of Kluyveromyces marxianus freeze-dried inoculum, which can solve problems such as tolerance differences, and achieve the effects of long storage time, simple ingredients and convenient use.

Active Publication Date: 2017-08-11
XINJIANG TIANRUN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Differences in yeast strains lead to differences in strain tolerance to desiccation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] High Density Culture:

[0025] a, preparation of seed liquid: streak Kluyveromyces marxe on the YEPD medium plate, place it in a 28° C. incubator and cultivate it for 1 day, pick the plump strains and streak again on the YEPD medium plate, Place the culture in a 28°C incubator for 1 day, then insert the strain into the YEPD liquid medium, and culture in a shaker flask at a temperature of 28°C and 160r / min overnight to obtain a seed liquid;

[0026] b. Preparation of fermentation medium: Weigh 67.37g of sucrose, 29.7g of yeast extract powder, 15.61g of corn steep liquor, 4.13g of potassium dihydrogen phosphate, and 0.3g of magnesium sulfate, dissolve them in 1L of distilled water, mix well, and the temperature is 121°C Sterilize for 20 minutes;

[0027] c. High-density fermentation culture: the seed liquid obtained in step a is inserted into the fermentation medium after step b is sterilized and cooled, and the inoculum size is 3×10 6 cfu / mL, the culture temperature is...

Embodiment 2

[0033] High Density Culture:

[0034] a, preparation of seed liquid: mark Kluyveromyces marxe on the YEPD medium plate, place it in a 30° C. incubator and cultivate it for 2 days, pick the full bacterial strain and mark it on the YEPD medium plate again, Place the culture in an incubator at a temperature of 30°C for 2 days, then insert the strain into the YEPD liquid medium, and culture in a shaker flask at a temperature of 30°C and 180r / min overnight to obtain a seed liquid;

[0035] b. Preparation of fermentation medium: Weigh 67.37g of sucrose, 29.7g of yeast extract powder, 15.61g of corn steep liquor, 4.13g of potassium dihydrogen phosphate, and 0.3g of magnesium sulfate, dissolve them in 1L of distilled water, mix well, and the temperature is 121°C Sterilize for 20 minutes;

[0036] c. High-density fermentation culture: the seed liquid obtained in step a is inserted into the fermentation medium after step b is sterilized and cooled, and the inoculum size is 3×10 6 cfu / ...

Embodiment 3

[0042] High Density Culture:

[0043] a, preparation of seed liquid: mark Kluyveromyces marxe on the YEPD medium plate, place it in a 29° C. incubator and cultivate it for 2 days, pick the full bacterial classification and mark it on the YEPD medium plate again, Place the culture in an incubator at a temperature of 29°C for 2 days, then insert the strain into the YEPD liquid medium, and culture in a shaker flask at a temperature of 29°C and 170r / min overnight to obtain a seed liquid;

[0044] b. Preparation of fermentation medium: Weigh 67.37g of sucrose, 29.7g of yeast extract powder, 15.61g of corn steep liquor, 4.13g of potassium dihydrogen phosphate, and 0.3g of magnesium sulfate, dissolve them in 1L of distilled water, mix well, and the temperature is 121°C Sterilize for 20 minutes;

[0045] c. High-density fermentation culture: the seed liquid obtained in step a is inserted into the fermentation medium after step b is sterilized and cooled, and the inoculum size is 3×10...

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Abstract

The invention relates to a method for preparing a freeze-dried microbial agent of kluyveromyces marxianus and application of the freeze-dried microbial agent. The freeze-dried microbial agent is prepared by the aid of high-density fermentation culture and freeze-drying technologies. The kluyveromyces marxianus is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms, called as CGMCC for short, on April 27th, 2010, the preservation number of the kluyveromyces marxianus is CGMCC NO.3781, and the strain number is A3. The method and the application have the advantages that the viable cell number of high-density culture solution of the kluyveromyces marxianus can reach 3.2*10<8> cfu / mL at least and is higher than culture results of YEPD (yeast peptone dextrose) culture media by 1.1 order of magnitude at least; zymocyte solution is centrifuged, then bacterial sludge is collected and is added into cryoprotectant solution, the freeze-dried microbial agent can be prepared by the aid of the vacuum freeze-drying technologies, protectants for the freeze-dried microbial agent comprise maltose and skimmed milk powder, and the freeze-dried viable bacterium rate can reach 75% at least; the freeze-dried microbial agent is high in bacterium content, long in storage time, convenient to use and free of pollution, is environmentally friendly and can be used for fermenting milk beer and cheese and making whey wine, fruit vinegar, fruit juice and the like, processes for preparing the freeze-dried microbial agent are simple, and the like.

Description

technical field [0001] The invention relates to a preparation method and application of a freeze-dried bacterial agent of Kluyveromyces marxe, belonging to the field of production of yeast products. Background technique [0002] As a yeast that widely exists in fermented milk, Kluyveromyces marx can not only ferment lactose, but also utilize galactose. During the fermentation process, it can be used alone or together with lactic acid bacteria to ferment milk, giving the product a unique flavor and nutritional properties. At present, the application of Kluyveromyces marxis at home and abroad is mainly focused on the research and development of new dairy products and the preparation of flavor substances such as esters, carboxylic acids, alcohols, and isoamyl acetate. The invention patent applied by Xinjiang Tianrun Biotechnology Co., Ltd. <Preparation of a high-quality yeast and its milk beer beverage> (Patent No. ZL201010256427.X) announced the use of Kluyveromyces mar...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/04C12C12/00C12C5/00C12R1/645
CPCC12C5/00C12C12/006C12C2200/01C12N1/04C12N1/16
Inventor 刘朋龙王亮胡曼刘让胡刚尤宏罗晓红詹涛李阳
Owner XINJIANG TIANRUN BIOTECH
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