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Microbial strain for water quality purification and fungicide

A technology for water purification and microbial bacteria, applied in the field of microorganisms, can solve the problems of harsh lighting and nutrient substrate requirements, slow growth of photosynthetic bacteria strains, high application cost of photosynthetic bacteria, etc. Loss and water pollution, reducing the effect of the breeding of harmful substances

Active Publication Date: 2016-06-01
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the main obstacle restricting the large-scale application of photosynthetic bacteria is that the existing photosynthetic bacteria strains generally grow slowly, and it usually takes more than 7 days to reach a certain concentration of bacteria, and the requirements for light and nutrient substrates are harsh. Easy to be polluted by miscellaneous bacteria, resulting in relatively high cost of photosynthetic bacteria application

Method used

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  • Microbial strain for water quality purification and fungicide
  • Microbial strain for water quality purification and fungicide
  • Microbial strain for water quality purification and fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Breeding and Physiological and Biochemical Characteristics of Strain HL

[0045] (1) The breeding method of Rhodopseudomonas strain HL, the steps are as follows:

[0046] 1) Grind and suspend 10g of the sediment from the fish pond in Xinjie Village, Yongan Town, Shuangliu District, Sichuan, and suspend it in 90mL sterile distilled water, shake evenly, boil for 15min, cool down and then dilute in gradient, and spread it on the screening medium, at 30°C Under culture 72h, obtain bacterium colony, the composition of screening medium is photosynthetic bacteria solid medium (g / L) is: yeast extract 1g, MgCl 2 0.2g, NH 4 Cl1g, K 2 HPO 4 0.5g, NaCl1g, NaHCO 3 1g, add water to 1000mL, adjust pH to 6.8.

[0047] 2) Select 6 bacterial colonies with bright red colonies, vigorous growth, and smooth surface. Under the same culture conditions, these 6 bacterial strains are repeatedly streaked and cultured for more than three times to obtain pure bacterial strains, which are inocu...

Embodiment 2

[0053] Preparation of strain HL microbial inoculum.

[0054] (1) Prepare the solid slant medium after high-temperature sterilization (121°C, 30 minutes) for later use. The formula of the solid slant medium is: 3g of yeast extract, 3g of peptone, CaCl 2 0.3g, MgSO 4 ·7H 2 O0.5g, agar 15-20g, add water to 1000mL, adjust pH to 6.8.

[0055] (2) The strain HL was inserted into the sterilized (121° C., 30 minutes) solid slant culture medium to be activated for later use.

[0056] (3) Quantify the liquid medium that has been sterilized at high temperature (121°C, 30 minutes) for later use. The formula of the liquid medium is: yeast extract 1g, MgCl 2 0.2g, NH 4 Cl1g, K 2 HPO 4 0.5g, NaCl1g, NaHCO 3 1g, add water to 1000mL, adjust pH to 6.8.

[0057] (4) Insert the activated strain HL into the liquid culture medium for shaking (30°C, 100 rpm) and cultivate for 72 hours, then adjust the count of the cultured bacterial liquid, and adjust the bacterial content of the HL strain t...

Embodiment 3

[0060] Preparation of microbial complex agents.

[0061] A, the preparation method of liquid microbial composite bacterial agent:

[0062] (1) Insert strains HL and LQ into solid slant medium for activation respectively, and the components of the solid slant medium are: yeast extract 3g, peptone 3g, CaCl 2 0.3g, MgSO 4 .7H 2 O0.5g, agar 15-20g, add water to 1000mL, adjust pH to 6.8.

[0063] (2) The sterilized liquid medium is quantitatively used for standby, and the liquid medium components are: yeast extract 1g, MgCl 2 0.2g, NH 4 Cl1g, K 2 HPO 4 0.5g, NaCl1g, NaHCO 3 1g, add water to 1000mL, adjust pH to 6.8.

[0064] (3) Insert the two activated strains into the sterilized liquid culture medium for microbial culture: the medium sterilization treatment method is: high-pressure steam sterilization at 121°C for 30 minutes; the microbial culture method is: 30°C , Anaerobic culture under 2000Lx light for 3-6d.

[0065] (4) adjust the counts of each bacterial solution t...

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Abstract

The invention relates to a microbial strain and a fungicide and belongs to the technical field of microorganisms.The invention provides a photosynthetic bacterial strain HL which is identified as red pseudomonas (Rhodopseudomonas sp.) CGMCC No.11874.The strain is quick to grow and propagate, high in bacterial concentration and good in sundry bacterium contamination resistance capability and can degrade and remove various pollutants under the lighting or dark condition.The invention further provides a microbial fungicide containing the strain and a compound fungicide papered by the strain and another photosynthetic bacterial strain.The microorganisms can well grow in treated wastewater adopting the fungicide, occupy beneficial ecological niches and inhibit the growth and propagation of pathogenic microorganisms.

Description

technical field [0001] The invention relates to a microbial strain and a bacterial agent, in particular to a microbial strain and a bacterial agent for purifying various sewage water, and belongs to the technical field of microorganisms. Background technique [0002] With the rapid development of my country's industry and the improvement of people's living standards, more and more industrial wastewater (such as heavy metal wastewater, petroleum wastewater, papermaking wastewater) and domestic sewage (such as catering industry oily wastewater, aquaculture wastewater) are produced in large quantities. After the above wastewater is discharged into the water body, it will cause great harm to the water body and the ecological system of the environment. In severe cases, it will destroy the ecological balance of the water body. badly damaged. Therefore, the water crisis caused by water environmental pollution has seriously restricted the development of the national economy and aff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/20
CPCC02F3/34C02F2101/20C12N1/20C12N1/205C12R2001/01
Inventor 闫志英袁月祥许力山廖银章李志东李东刘晓风
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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