Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compound microbial preparation and culture method and application thereof

A technology of compound microorganisms and cultivation methods, applied in the field of compound microorganism preparations and their cultivation, can solve the problems that a single microorganism cannot be completed and can only be carried out weakly, so as to improve biomass and physiological metabolism functions, save manpower and material resources, and grow high rate effect

Pending Publication Date: 2020-05-22
广东中科无抗生态科技有限公司
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in long-term experiments and production practices, people have found that many important biochemical processes cannot be completed by a single microorganism or can only be carried out weakly, and must be completed by co-cultivation of two or more microorganisms.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound microbial preparation and culture method and application thereof
  • Compound microbial preparation and culture method and application thereof
  • Compound microbial preparation and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] (1) Strain activation culture: Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium bifidum, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Pseudomonas utilis in the cryopreservation tube Trichotillomyces were activated in 18×180mm test tube slant respectively, activated three times, and then inoculated in 90mm petri dishes for purification and culture, and then cultured in 18×180mm test tube slant respectively to obtain Lactobacillus acidum, Lactobacillus plantarum, Bifidus Bifidobacterium, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Candida utilis activated strains; Among them, the specific culture conditions are: Lactobacillus acidophilus, Lactobacillus plantarum 37 ℃ facultative Anaerobic culture for 2 days; Bifidobacterium bifidum 37 ℃ anaerobic culture for 3 days, Bacillus subtilis and Bacillus licheniformis 30 ℃ aerobic culture for 2 days; Cultiva...

Embodiment 2

[0081] (1) Strain activation culture: Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium bifidum, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Pseudomonas utilis in the cryopreservation tube Trichotillomyces were activated in 18×180mm test tube slant respectively, activated three times, and then inoculated in 90mm petri dishes for purification and culture, and then cultured in 18×180mm test tube slant respectively to obtain Lactobacillus acidum, Lactobacillus plantarum, Bifidus Bifidobacterium, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Candida utilis activated strains; Among them, the specific culture conditions are: Lactobacillus acidophilus, Lactobacillus plantarum 37 ℃ facultative Anaerobic culture for 3 days; Bifidobacterium bifidum 37 ℃ anaerobic culture for 3 days, Bacillus subtilis and Bacillus licheniformis 30 ℃ aerobic culture for 2 days; Culture...

Embodiment 3

[0097] (1) Strain activation culture: Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium bifidum, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Pseudomonas utilis in the cryopreservation tube Trichotillomyces were activated in 18×180mm test tube slant respectively, activated three times, and then inoculated in 90mm petri dishes for purification and culture, and then cultured in 18×180mm test tube slant respectively to obtain Lactobacillus acidum, Lactobacillus plantarum, Bifidus Bifidobacterium, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Candida utilis activated strains; Among them, the specific culture conditions are: Lactobacillus acidophilus, Lactobacillus plantarum 37 ℃ facultative Anaerobic culture for 3 days; Bifidobacterium bifidum 37 ℃ anaerobic culture for 3 days, Bacillus subtilis and Bacillus licheniformis 30 ℃ aerobic culture for 2 days; Culture...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of microbial culture, and in particular to a compound microbial preparation and a culture method and application thereof. The culture method includes: adopting a mixed culture technology, respectively subjecting Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium bifidum, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae and Candida utilis strains to activation, liquid strain cultivation, composite strain cultivation and finished product cultivation to obtain the compound microbial preparation. The preparation can be used for pig breeding, and has effects of promoting growth, helping digestion, resisting diseases, increasing animal body weight and reducing feed consumption. The methodimproves biomass and physiological metabolic function of microorganisms, makes up for shortcomings of independent culture, uses symbiosis, partial symbiosis, etc. to promote growth of compound microorganisms, and has many obvious advantages compared with a traditional pure culture technology.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a compound microorganism preparation and its cultivation method and application. Background technique [0002] Mixed culture refers to the culture of two or more microorganisms, which is a new development of pure culture technology and a new culture technology that does not require complicated DNA recombination in vitro but can achieve similar effects. The use of microorganisms by humans has gone through two stages: natural mixed culture and pure culture. Pure culture enables researchers to get rid of the complicated situation of coexistence of multiple microorganisms, and can study single-purpose strains without interference, thus enriching our understanding of microorganisms. Knowledge of morphological structure, physiological and genetic traits. However, in the long-term experiment and production practice, people have found that many important biochemical pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/18A01K67/02A23K50/30A23K10/18C12R1/23C12R1/25C12R1/01C12R1/125C12R1/10C12R1/38C12R1/865C12R1/72
CPCC12N1/20C12N1/16C12N1/18A01K67/02A23K50/30A23K10/18
Inventor 莫艳华姚康马国华黄海红
Owner 广东中科无抗生态科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products