Compound microbial preparation and culture method and application thereof
A technology of compound microorganisms and cultivation methods, applied in the field of compound microorganism preparations and their cultivation, can solve the problems that a single microorganism cannot be completed and can only be carried out weakly, so as to improve biomass and physiological metabolism functions, save manpower and material resources, and grow high rate effect
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Embodiment 1
[0065] (1) Strain activation culture: Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium bifidum, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Pseudomonas utilis in the cryopreservation tube Trichotillomyces were activated in 18×180mm test tube slant respectively, activated three times, and then inoculated in 90mm petri dishes for purification and culture, and then cultured in 18×180mm test tube slant respectively to obtain Lactobacillus acidum, Lactobacillus plantarum, Bifidus Bifidobacterium, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Candida utilis activated strains; Among them, the specific culture conditions are: Lactobacillus acidophilus, Lactobacillus plantarum 37 ℃ facultative Anaerobic culture for 2 days; Bifidobacterium bifidum 37 ℃ anaerobic culture for 3 days, Bacillus subtilis and Bacillus licheniformis 30 ℃ aerobic culture for 2 days; Cultiva...
Embodiment 2
[0081] (1) Strain activation culture: Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium bifidum, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Pseudomonas utilis in the cryopreservation tube Trichotillomyces were activated in 18×180mm test tube slant respectively, activated three times, and then inoculated in 90mm petri dishes for purification and culture, and then cultured in 18×180mm test tube slant respectively to obtain Lactobacillus acidum, Lactobacillus plantarum, Bifidus Bifidobacterium, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Candida utilis activated strains; Among them, the specific culture conditions are: Lactobacillus acidophilus, Lactobacillus plantarum 37 ℃ facultative Anaerobic culture for 3 days; Bifidobacterium bifidum 37 ℃ anaerobic culture for 3 days, Bacillus subtilis and Bacillus licheniformis 30 ℃ aerobic culture for 2 days; Culture...
Embodiment 3
[0097] (1) Strain activation culture: Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium bifidum, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Pseudomonas utilis in the cryopreservation tube Trichotillomyces were activated in 18×180mm test tube slant respectively, activated three times, and then inoculated in 90mm petri dishes for purification and culture, and then cultured in 18×180mm test tube slant respectively to obtain Lactobacillus acidum, Lactobacillus plantarum, Bifidus Bifidobacterium, Bacillus subtilis, Bacillus licheniformis, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Candida utilis activated strains; Among them, the specific culture conditions are: Lactobacillus acidophilus, Lactobacillus plantarum 37 ℃ facultative Anaerobic culture for 3 days; Bifidobacterium bifidum 37 ℃ anaerobic culture for 3 days, Bacillus subtilis and Bacillus licheniformis 30 ℃ aerobic culture for 2 days; Culture...
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