Anti-stress microbiological preparation for prawns and preparation method of anti-stress microbiological preparation for prawns

A microbial preparation and anti-stress technology, which is applied in the field of prawn feed additives, can solve the problems of being easily startled, jumping into the pond, broken whiskers, and single stress response of prawns.

Inactive Publication Date: 2020-02-04
仝丹丹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 3) Extremely frightened and jumped into the pond;
[0008] 4) prone to yellow gills, black gills, broken whiskers
But because in the process of prawn breeding, transportation or sales, the prawn stress response caused is usually not a single existence, and the existing technology for coping with prawn farming cannot meet the needs of the existing prawn industry development. Stress for further research

Method used

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  • Anti-stress microbiological preparation for prawns and preparation method of anti-stress microbiological preparation for prawns
  • Anti-stress microbiological preparation for prawns and preparation method of anti-stress microbiological preparation for prawns
  • Anti-stress microbiological preparation for prawns and preparation method of anti-stress microbiological preparation for prawns

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Preparation of microbial preparations for the anti-stress response of prawns:

[0099] The microbial preparation includes complex microbial bacteria and total culture medium.

[0100] The composite microbial bacteria include the following strains in weight ratio: 50% of Bacillus subtilis, 20% of Saccharomyces cerevisiae, 10% of Lactobacillus casei and 20% of Rhodopseudomonas palustris.

[0101] Wherein, the brewer's yeast (Sac-charomyces cerevisiae), Bacillus subtilis (Bacillus subtilis), Lactobacillus casei (Lactobacillus casei) and Rhodopseudomonas palustris (Rhodop seudanonas palustris) used in this example are all provided by China Agricultural Introduced by the Microbial Culture Collection Center (ACCC).

[0102]The medium is composed of bean cake powder, corn flour, corn starch, urea, ammonium sulfate, potassium dihydrogen sulfate, disodium hydrogen phosphate, magnesium sulfate, and rice bran powder, and its weight ratio is 8% of bean cake powder and 15% of corn ...

Embodiment 2

[0113] Preparation of Microbial Preparations for Shrimp Anti-Stress Response:

[0114] The microbial preparation includes complex microbial bacteria and total culture medium.

[0115] The composite microbial bacteria include the following strains by weight: 30% of Bacillus subtilis, 20% of Saccharomyces cerevisiae, 20% of Lactobacillus casei and 20% of Rhodopseudomonas palustris.

[0116] Wherein, the brewer's yeast (Sac-charomyces cerevisiae), Bacillus subtilis (Bacillus subtilis), Lactobacillus casei (Lactobacillus casei) and Rhodopseudomonas palustris (Rhodop seudanonas palustris) used in this example are all provided by China Agricultural Introduced by the Microbial Culture Collection Management Center (ACCC)

[0117] The medium is composed of bean cake powder, corn flour, corn starch, urea, ammonium sulfate, potassium dihydrogen sulfate, disodium hydrogen phosphate, magnesium sulfate, rice bran powder, and its weight ratio is 20% of bean cake powder, 25% of corn flour %...

Embodiment 3

[0128] Preparation of Microbial Preparations for Shrimp Anti-Stress Response:

[0129] The microbial preparation includes complex microbial bacteria and total culture medium.

[0130] The composite microbial bacteria include the following strains by weight: 40% of Bacillus subtilis, 30% of Saccharomyces cerevisiae, 10% of Lactobacillus casei and 20% of Rhodopseudomonas palustris.

[0131] Wherein, the brewer's yeast (Sac-charomyces cerevisiae), Bacillus subtilis (Bacillus subtilis), Lactobacillus casei (Lactobacillus casei) and Rhodopseudomonas palustris (Rhodop seudanonas palustris) used in this example are all provided by China Agricultural Introduced by the Microbial Culture Collection Management Center (ACCC)

[0132] The medium is composed of bean cake powder, corn flour, corn starch, urea, ammonium sulfate, potassium dihydrogen sulfate, disodium hydrogen phosphate, magnesium sulfate, and rice bran powder, and its weight ratio is 14% of bean cake powder and 20% of corn f...

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Abstract

The invention discloses an anti-stress microbiological preparation for prawns. The microbiological preparation comprises composite microorganism bacteria, wherein the composite microorganism bacteriacomprise the following strains in percentage by weight of 20-60% of bacillus subtilis, 10-50% of beer microzyme, 3-40% of lactobacillus casei and 10-50% of rhodopseudomonas palustris. The invention further discloses a preparation method of the microbiological preparation. The preparation method comprises the steps of respectively inoculating a corresponding culture medium with four strains, and performing propagation culture to obtain strains; and mixing the strains in a proportion to obtain a composite microorganism bacterium stock solution, then performing inoculating in a total culture medium, and then performing fermentation to prepare the required microbiological preparation. The microbiological preparation disclosed by the invention can be added to any prawn feeds, wherein the microbiological preparation can rapidly remove.OH free radicals in prawns, and can reduce the cortisol and blood sugar concentration in serum, so as to reduce the influence of the stress response of the prawns on organisms, and improve the resistance of the prawns.

Description

technical field [0001] The invention relates to a prawn feed additive, in particular to a microbial preparation for anti-stress response of prawns which can be added to prawn feed and a preparation method thereof. Background technique [0002] As people's consumption level is getting higher and higher, the requirements for the diversity and nutritional value of food are also increasing. Prawns are deeply loved by consumers because of their rich nutrition, soft meat, easy digestion, rich in magnesium, and beneficial to the cardiovascular system. Among them, Penaeus vannamei is one of the three largest prawn species with the highest aquaculture output in the world. First, due to its delicious meat quality, it is suitable for desalination farming, and has gradually become one of the main shrimp farming species in my country's aquatic products. [0003] In order to improve the productivity of prawn farming, intensive farming, transportation and sales are all adopted in the exist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18A23K10/18A23K50/80C12R1/125C12R1/865C12R1/245C12R1/01
CPCA23K10/18A23K50/80C12N1/18C12N1/20Y02A40/818
Inventor 仝丹丹
Owner 仝丹丹
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