74 results about "Cortisol synthesis" patented technology

The invention relates to the technical field of medical detection, in particular to a method for detecting aldosterone, cortisol, deoxycorticosterone and cortisone in plasma through LC-MS / MS. The method comprises the following steps: (a) mixing a plasma sample with an internal standard solution to obtain a mixed solution; (b) adding the mixed solution into the magnetic bead suspension, sequentially carrying out adsorption, weak washing, strong washing and elution, and collecting an eluent; (c) carrying out liquid nitrogen blowing and redissolving on the eluent, and then carrying out LC-MS / MS detection; wherein the surfaces of the magnetic beads are modified with steroid adsorption groups, and the target detection objects are aldosterone, cortisol, deoxycorticosterone and cortisone. The detection method is convenient and simple, the detection efficiency is higher than that of a traditional method, and a feasible solution is provided for promoting popularization and application of the LC-MS / MS technology in clinical practice.

Relacorilant is useful in the treatment of hypercortisolism and cancer. Many drugs useful in treating hypercortisolism or cancer are metabolized by CYP2C9 enzymes. The effects of concomitant administration of relacorilant and a CYP2C9 substrate are disclosed herein.Relacorilant potently inhibited CYP2C9 in an in vitro test, indicating that co-administration of relacorilant and a CYP2C9 substrate would be expected to increase the CYP2C9 substrate plasma exposure more than five-fold in vivo. Significant reductions in CYP2C9 substrate doses would be expected to be required when administered with relacorilant.Surprisingly, no such increase in plasma exposure was seen in human studies. Applicant discloses that relacorilant may be safely co-administered with unmodified doses of a CYP2C9 substrate such as, e.g., tolbutamide, glimepiride, and glipizide. Relacorilant and unmodified doses of CYP2C9 substrate such as tolbutamide, glimepiride, and glipizide may be co-administered to treat hypercortisolism, or may be co-administered to a cancer patient.

The present invention relates to an oral extract composition comprising Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) extract composition, and its process of preparation. Also relates to the oral extract composition formulation comprising with synergistic combinations of the Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) in suitable doses and at least one nutraceutically and / or pharmaceutically accepted excipient for, improvement, and maintenance of women health more preferably in improving of hot flashes, night sweats, poor sleep, anxiety, sexual dysfunction, mood changes, Genitourinary symptoms in overall improving the quality of life, serum estradiol, progesterone, follicle stimulating hormone (FSH), and cortisol during the period of menopause (perimenopause and menopause). The oral extract composition formulation comprising with synergistic combinations of the Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) in suitable doses and at least one nutraceutically and / or pharmaceutically accepted excipient is used for improving Brain-derived neurotrophic factor (BDNF) levels in women during the period of menopause. The present invention, further related to the extraction process of Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.), also provides the process for the preparation of formulation composition. This invention further relates to the extraction process from Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) either as individual herbs or co-extraction of the two herbs or mixing the extracts at various stages of the individual extracts.

The invention discloses a wearable molecular imprinting sensor and application thereof in cortisol detection, and belongs to the technical field of electrochemical analysis and detection. The molecular imprinting sensor sequentially comprises a sweat absorbing pad, a three-electrode system, an annular polydimethylsiloxane mold and a medical bonding patch from the skin contact surface to the outside, the front surface of the sweat-absorbent pad is in contact with the skin, and KCl and K3 [Fe (CN) 6] are adsorbed on the back surface of the sweat-absorbent pad; the three-electrode system takes an MIP / AuNWs / PET sensor as a working electrode, a platinum wire as a counter electrode and a silver / silver chloride electrode as a reference electrode; the annular polydimethylsiloxane mold and the medical adhesive patch form a sweat collection chamber. The molecular imprinting sensor based on in-situ growth of gold nanowires (AuNWs) on the porous PET film is used for rapidly detecting the cortisol level in human sweat in real time, natural receptor ligand interaction is simulated through the molecular imprinting technology, and high-specificity recognition of cortisol is achieved.

This invention relates to the field of in vitro diagnostic technology, and more particularly to a method for determining cortisol levels and its application. This invention provides a pretreatment reagent combination for liquid chromatography, a method for pretreating urine samples in liquid chromatography, and a method for detecting cortisol levels in urine. The method for detecting cortisol levels in urine samples provided by this invention offers convenient and rapid pretreatment, and through method validation, demonstrates excellent performance indicators.

The present application relates to a kind of pituitary adenoma simulation teaching method, one aspect, in the case of unified time axis, with the volume of pituitary adenoma as the basis, the hormone level change of corresponding hormone species and visual change condition are associated, can clearly know the curve and numerical value of GH, PRL, ACTH, cortisol and other key hormones, the condition of real-time change with tumor state change, and the real-time synchronization of visual field change and the increase and decrease of tumor size, strengthen the understanding of "space-occupied effect-dysfunction" correlation of the trainee, and the degree of oppression of pituitary adenoma to surrounding tissue is quantified by pressure sensor, the compression phenomenon of pituitary adenoma volume change is directly reflected;Another aspect, for a large number of hormone species data, through the preset data ROI search algorithm, the position of data weak fluctuation can be accurately found, and the corresponding volume change moment of pituitary adenoma helps trainee to locate quickly key time point, improves learning efficiency.

The invention provides a compound feed additive for relieving stress response of marine fishes, which is prepared by the following steps: mixing raw materials such as hermetia illucens protein and the like to prepare a premix, and embedding the premix by using a lac-glycyrrhizic acid system to prepare lac microcapsules; then modifying the fucoidin by adopting glycyrrhizin and gamma-linolenic acid, embedding rhodosporidium palustris and bacillus pumilus by using the fucoidin to prepare compound bacterium nano-particles, and finally mixing the lac microcapsules and the compound bacterium nano-particles in proportion. According to the invention, excessive secretion of cortisol of marine fish can be effectively inhibited, hermetia illucens protein provides rich nutrition and reduces muscle decomposition, and compound microorganisms are beneficial to maintaining intestinal microecological balance and improving the utilization rate of nutrition. The prepared compound feed additive can be directly splashed into a culture or transportation water body or added into a feed, so that the stress reaction of the marine fish is effectively relieved, the survival rate of the marine fish is increased, and powerful support is provided for culture of the marine fish.

The present application relates to a kind of anti-aging composition for promoting intercellular communication of skin cell and its application, the composition includes the following components: camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside, the weight ratio of the camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside is (1-10):(1-8):(1-6):(0.1-1).The anti-aging composition of the present application can promote the activity of skin communication cell, strengthen intercellular communication and interaction, fully exert the function of cell biology, achieve the effect of anti-aging;And inhibit the damage of cortisol to skin barrier, achieve the effect of repair and emotional stress relief, synergistic effect between components, can achieve the best wrinkle, firming, smoothing and repair effect.

The invention provides a polianthes tuberosa corm extract and a preparation method and application thereof.The preparation method includes the steps that fresh polianthes tuberosa corm and water are mixed and then smashed, then stirring extraction is conducted at the temperature of 50-70 DEG C, supernate is collected through centrifugation and filtered, and polyhydric alcohol with the volume concentration of 30-50% is added; and the polyol is 1, 3-butanediol or glycerol. According to the tuberose corm extract disclosed by the invention, by inhibiting TNF-alpha induced cortisol excessive secretion and promoting beta-ennorphin release, the dual effects of inhibiting stress and promoting soothing are realized, and the problems of skin inflammation, sensitivity, barrier damage and the like caused by psychological emotion stress are solved.

The invention discloses a composite electrode for rapidly detecting cortisol and application of the composite electrode in rapid detection of cortisol. The composite electrode comprises a laser-induced graphene substrate, a gold nano material layer modified on the laser-induced graphene substrate and a perfluorosulfonic acid polymer modified on the gold nano material layer. The composite electrode does not need an incubation binding step, is high in timeliness, improves the detection rate, can realize real-time analysis, and is stable in film-forming interface; the method is not influenced by the dispersity of a catalytic material, so that the difficulty of the product performance consistency requirement in a quality control link in industrial production is greatly reduced; the electrocatalytic analysis of cortisol can be realized in the PBS buffer solution, and the application range is wider.

The invention discloses a method for detecting cortisol, testosterone and dehydroepiandrosterone in saliva, and belongs to the field of biological medicine. The technical problem to be solved is to provide a method for accurately detecting cortisol, testosterone and dehydroepiandrosterone in saliva. According to the technical scheme, the method for detecting cortisol, testosterone and dehydroepiandrosterone in saliva is characterized in that a magnetic bead method is adopted for sample pretreatment, a to-be-detected sample is obtained through magnetic bead activation, balancing, sample loading, leaching, elution and nitrogen blowing redissolution, and the to-be-detected sample is detected through a liquid chromatography-tandem mass spectrometry method. The contents of cortisol, testosterone and dehydroepiandrosterone in the sample to be detected are obtained. The detection method is accurate and reliable in detection result, simple, convenient and efficient to operate, and meets industrial practical requirements.

The application provides a wearable sweat micro-fluidic analysis chip and a preparation method and application thereof. The wearable sweat micro-fluidic analysis chip is sequentially provided with a sweat inlet layer 1, a branch channel layer 3 and a cover layer 5 from top to bottom. The sweat inlet layer is provided with a sweat inlet 6. The branch channel layer 3 is provided with a detection chamber 10, a channel 17 and a valve chamber 9. The sweat inlet 6, the valve chamber 9, the channel 17 and the detection chamber 10 are sequentially communicated. The cover layer 5 is provided with a sweat outlet 14. A water droplet type polymer valve is placed in the valve chamber 9. The opening time of the water droplet type polymer valve can be flexibly controlled by simply adjusting the polymer concentration. The sweat sample can be sequentially collected, and the contact time of the sweat sample and the detection reagent can be accurately controlled. A reliable premise is provided for subsequent accurate analysis. The application can be applied to the detection of cortisol and the detection of uric acid.

A Lacticaseibacillus rhamnosus MBP01 and a combination thereof for preventing and treating irritable bowel syndrome are provided, which relates to the technical field of microorganism. A deposit number of the Lacticaseibacillus rhamnosus MBP01 is GDMCC NO: 64725. The MBP01 can improve a weight loss trend of IBS model mice, increase food intake and feces water content, alleviate colon tissue damage, downregulate expression levels of IL-1β, TNF-α, PAR-2, and serum CORT and increase an expression level of IL-10. MBP01 with a high concentration can improve an expression level of OCC. Therefore, MBP01 has a certain positive regulatory effect on the intestinal function of IBS model mice and can effectively prevent and treat irritable bowel syndrome.

The invention relates to the technical field of computers, and discloses an endocrine metabolism index real-time processing method based on edge calculation. The method comprises the following steps: acquiring time sequence data of blood glucose, insulin, cortisol and thyroid stimulating hormone collected by wearable equipment; constructing a lightweight linear state space model of the diagonal dominant sparse state transition matrix; initializing a noise covariance matrix; iterative Kalman filtering based on a sparse matrix inversion algorithm is executed; the observation noise covariance is dynamically adjusted according to the residual error; and when the filtering result exceeds a clinical critical value, triggering local early warning and uploading to the cloud. According to the scheme, the single filtering delay is controlled within 80 milliseconds while the numerical stability is guaranteed, and the early warning timeliness and the system energy efficiency are improved.

This invention discloses a naturally formed nutritional licking brick specifically for musk deer and its preparation method. Using calcium silicate as the skeleton material, it is compounded with yeast culture powder, bentonite, compound probiotics, sweeteners, and flavoring agents. The resulting licking brick continuously releases soluble nutrients and active bacteria during the musk deer's licking process. The specific combination of probiotics and yeast culture powder components effectively improves the intestinal immunity of captive musk deer, significantly reduces intestinal inflammation, and simultaneously lowers cortisol levels in feces, alleviating stress responses. Using yeast culture powder, calcium silicate, and bentonite as a composite coagulant, the licking brick prepared by mold casting exhibits optimal moisture resistance during storage. Furthermore, the licking brick has a smooth appearance, a compact and sturdy structure, excellent molding performance, and retains its structural integrity even after immersion in water, making it suitable for preparing products related to improving the intestinal health and immunity of musk deer.

The invention is suitable for the technical field of biological medicine, and provides an active composition for relieving fatigue and application of the active composition. The composition is prepared from a silkworm chrysalis-derived mitochondrial repair peptide, a chickpea-derived neuromodulation peptide, a haematococcus pluvialis-derived phagocytosis peptide, a phosphatidylcholine-lipoic acid conjugate, a whey protein-chitosan copolymer and an ergothioneine-selenium compound in a synergistic manner. Through the synergistic effect of four mechanisms of energy metabolism activation, endocrine balance, mitochondrial autophagy repair and oxidative stress removal, physiological fatigue and stress fatigue are effectively and synchronously relieved. After targeted modification, the utilization rate of macamides is remarkably increased, and the composition can remarkably prolong exercise tolerance, reduce cortisol level, promote GABA synthesis, recover mitochondrial function and greatly shorten fatigue recovery time.

The invention discloses application of a mulberry root bark extract, and particularly relates to application of the mulberry root bark extract in preparation of a product for improving skin cortisol unbalance caused by pressure stress. The mulberry root bark extract provided by the invention can improve skin problems caused by excessive transformation of cortisol due to pressure stress, and improve the expression quantity of skin collagen, skin barrier related protein and skin basement membrane related protein, so that skin barrier damage, skin sensitivity, skin aging, skin aging and the like caused by emotional pressure are effectively relieved. Wrinkles are generated; the skin elasticity is reduced and the like.

Devices and methods for measurement of a hydroxysteroid analyte concentration in a host is provided, including a sensor housing, the sensor housing comprising an implantable analyte sensing portion configured to generate a signal associated with a concentration of a hydroxysteroid analyte, the analyte sensing portion comprising at least one (NAD(P)+)- dependent hydroxysteroid dehydrogenase enzyme; and at least one NAD(P)H oxidase. Detecting a signal corresponding to the hydroxysteroid analyte of the implantable portion after implantation of the implantable portion is also provided.

The invention relates to the technical field of specific therapeutic activity of pharmaceutical preparations, in particular to a PRP administration concentration evaluation method for joint movement injury repair, which comprises the following steps: obtaining an inflammation comprehensive repair expression according to the change condition of multiple C-reactive protein levels before the last PRP administration; according to the change condition of the joint swelling degree in a time period between two adjacent C-reactive protein levels, joint swelling repair characteristics are obtained; determining the change correlation between the arthrocele repair characteristics and the C-reactive protein level, and combining the arthrocele repair characteristics and the inflammation comprehensive repair expression to obtain comprehensive repair effect characteristics; obtaining hormone level deviation characteristics according to multiple abnormal conditions of cortisol hormone levels; according to the comprehensive repair effect characteristics and the hormone level deviation characteristics, the next PRP administration concentration is adjusted, the accuracy and reliability of determining the PRP administration concentration are effectively improved, and therefore the overall effect of joint injury repair is improved.

This invention discloses a sensor for detecting glucose, cortisol, sodium ions, and pH, and its preparation method, belonging to the field of sensor technology. It includes a substrate layer, a detection module disposed above the substrate layer, and the detection module comprising a glucose detection module, a cortisol detection module, a sodium ion detection module, and a pH detection module; as well as a biomimetic microfluidic unit and a biomimetic sweat-guiding fabric layer. This invention achieves simultaneous real-time detection of multiple parameters in sweat, including glucose, cortisol, sodium ion concentrations, and pH, through the glucose, cortisol, sodium ion, and pH detection modules. Simultaneously, the biomimetic sweat-guiding fabric layer constructs a wettability gradient between the hydrophilic and hydrophobic sides, enabling unidirectional moisture-guiding transport of sweat from the skin side to the detection side. This effectively improves sweat collection efficiency, inhibits sweat backflow, and enhances the accuracy of glucose, cortisol, sodium ion concentration, and pH detection in sweat.

This invention provides a method for preparing and applying a LIG molecularly imprinted electrochemical sensor based on the synergistic enhancement of hydrophobic comonomers N-MCz-PABA and AuNPs. Using LIG as the substrate electrode, the invention copolymerizes the hydrophobic monomer N-MCz, the electropolymerizable monomer PABA, and AuNPs precursors onto the electrode surface via a one-step electropolymerization method, forming an N-MCz-PABA@Au molecularly imprinted composite film with both high-affinity recognition sites and high conductivity. This sensor utilizes the enhanced hydrophobic interaction provided by N-MCz to dominate the specific recognition of cortisol, and leverages the in-situ embedded AuNPs to improve interfacial electronic conductivity, combined with the exogenous redox probe K3[Fe(CN)6] to achieve signal transduction. Its detection linear range for cortisol is 0.01–100 μM, with a detection limit of 4.6 nM. This invention features a simple preparation process, low cost, high sensitivity, and good selectivity, providing an effective platform for the immediate and rapid detection of hydrophobic small molecules in complex biological matrices such as sweat.

The application discloses a tuberose extract and a preparation method and application thereof, and comprises pretreatment, extraction of oil-soluble extract, addition of the pretreated dry flower powder into a solvent, heating extraction, centrifugation, collection of supernatant, filtration, collection of liquid phase, and obtaining of the tuberose extract. The tuberose extract has the functions of antioxidation, inhibition of cortisol and lipid antioxidation, can efficiently remove free radicals at a lower concentration, significantly inhibit lipid peroxidation, help skin resist oxidative damage, effectively regulate cortisol level, and relieve endogenous and exogenous stress. The application has a mild process, extracts fat-soluble active ingredients of tuberose, avoids residue problems of volatile organic solvents, guarantees product safety, and provides a new multifunctional, high-activity and high-safety raw material for the cosmetic industry.

The invention belongs to the technical field of immunochromatography detection, and particularly relates to a time-resolved fluorescence immunochromatography test strip for acute stress syndrome detection as well as a preparation method and application of the time-resolved fluorescence immunochromatography test strip. An IgA antibody is introduced into a detection system to serve as a quality control or recognition element, and the detection specificity and stability are further enhanced. By utilizing the IgA antibody, not only can the compatibility of the reagent and the sample be improved, but also the signal strength can be enhanced through the multivalent binding characteristic of the IgA antibody, so that the detection sensitivity is remarkably improved. Therefore, the subtle fluctuation of cortisol and other hormones in the acute stress state can be more clearly captured, the physiological change of the stress reaction is more obviously reflected, and a more accurate, convenient and reliable tool is provided for early screening, auxiliary diagnosis and curative effect evaluation of mental and psychological diseases.

This invention discloses antibodies against cortisol or reagents and kits for detecting cortisol, relating to the field of antibodies. The anti-cortisol antibody disclosed in this invention includes a heavy chain complementarity-determining region and a light chain complementarity-determining region. This antibody provides an important source of raw materials for the detection of cortisol and exhibits good affinity or activity.

The invention discloses a homogeneous polysaccharide from Xiaoyao powder as well as a preparation method and application of the homogeneous polysaccharide. The homogeneous polysaccharide is composed of arabinose, galactose, mannose, glucose and fructose, and the weight-average molecular weight Mw of the homogeneous polysaccharide is 293.223 kDa. The polysaccharide is separated and purified from Xiaoyao powder through water extraction, alcohol precipitation, ion exchange column chromatography, gel column chromatography and other preparation methods. Experimental results show that the homogeneous polysaccharide can improve cortisol-induced zebra fish depression model behavioral performance and improve exercise activity, has anti-depression activity, and can be used for preparing drugs for treating or preventing depression.

Congenital adrenal hyperplasia (CAH) is a group of autosomal recessive disorders that result in enzyme deficiencies that alter the production of adrenal steroids, due to 21-hydroxylase deficiency (a condition in which little or no cortisol biosynthesis occurs). A method is provided for administering 4-(2-chloro-4-methoxy-5-methylphenyl)-N-[(1S)-2-cyclopropyl-1-(3-fluoro-4-methylphenyl)ethyl]-5-methyl-N-propa-2-inyl-1,3-thiazole-2-amine, or a pharmaceutically acceptable salt thereof, to adult subjects with congenital adrenal hyperplasia.

The invention discloses a sensing device for synchronously monitoring lactic acid and cortisol in sweat and a preparation method of the sensing device. The sensing device is composed of a flexible patch substrate, a bionic sweat management unit, a multi-mode electrochemical sensing unit and an intelligent analysis early warning unit, wherein the bionic sweat management unit, the multi-mode electrochemical sensing unit and the intelligent analysis early warning unit are integrated with the flexible patch substrate. The bionic unit adopts a flower-shaped micro-channel and hydrophilic and hydrophobic wettability gradient design, and passive self-driven capture, directional enrichment and discharge of sweat are realized based on Laplace pressure difference. The sensing unit adopts a four-electrode system, and the specific synchronous detection of lactic acid and cortisol is realized through a first working electrode and a second working electrode. The intelligent unit is internally provided with an algorithm, synchronously processes double-path signals, fuses and analyzes exercise metabolism and pressure level, and generates comprehensive health assessment and early warning. The preparation method comprises the steps of constructing the flower-shaped micro-channel and the wettability gradient, processing and modifying the four electrodes and performing system integrated packaging. According to the invention, passive, stable and synchronous monitoring and intelligent early warning of two indexes are realized.

The invention discloses application of a ganoderma lucidum extract in preparation of a product for improving skin problems, bidirectional regulation of skin homeostasis is realized through a dual-action mechanism, on one hand, conversion of corticosterone to cortisol is inhibited, and on the other hand, the skin homeostasis is inhibited; the problems of skin barrier injury, skin basement membrane injury, excessive sebum secretion, accelerated aging and the like caused by excessive cortisol are relieved; on the other hand, by inhibiting differentiation of macrophages induced by noradrenaline to inflammation phenotypes, the cortisol level is reduced, meanwhile, the skin inflammation response excited by early pressure is effectively controlled, and bidirectional regulation of skin homeostasis is achieved.

The application discloses a P450 enzyme mutant, a coding gene and application thereof in preparation of cortisone, and belongs to the technical field of bioengineering. The P450 enzyme mutant is obtained by amino acid mutation of CYP11B1 from Homo sapiens with an amino acid sequence shown as SEQ ID NO. 1, and the amino acid mutation site is at least one of S146V, H331D and L440F. The P450 enzyme mutant provided by the application has a higher soluble expression level in Escherichia coli than wild-type CYP11B1, the catalytic level of the constructed recombinant expression strain is higher, 11-deoxycortisol can be used as a substrate to prepare cortisone, the yield of the product is high, no by-product is generated, and the P450 enzyme mutant has a good industrial application prospect.

The application discloses a kind of Ni / Co / Fe ternary MOF electrochemical aptamer sensor preparation method and sweat cortisol detection application, belong to electrochemical sensor technical field.The PB nanoparticle is deposited as embedded electron medium, and the three-metal MOF with high conductivity and porous structure is modified as catalytic substrate, and then the cortisol specific aptamer is covalently fixed, and the signal transmission, electrocatalysis and molecular recognition are realized on single working electrode.The application does not need additional electron medium or enzyme label, realizes the real "reagent-free" detection;At the same time, due to the synergistic catalytic effect of multi-metal MOF and PB and the high selectivity of aptamer, the sensor shows excellent sensitivity, stability and anti-interference ability in complex sweat matrix.The application provides a feasible technical path for developing low-cost, wearable sweat cortisol continuous monitoring equipment, and has significant industrialization application prospect.