133 results about "Cortisol synthesis" patented technology

The invention belongs to the technical field of gestational diabetes diagnosis marker screening, and particularly relates to a biomarker for predicting or diagnosing gestational diabetes and application of the biomarker. The invention discloses a biomarker for predicting or diagnosing gestational diabetes mellitus, the biomarker is selected from one or more of hypoxanthine, cortisol, dihydrotachysterol and cycloleucine, and more preferably, the biomarker comprises hypoxanthine, cortisol, dihydrotachysterol, (3R, 4R)-D-ketolactone, creatine, cycloleucine and L-hydroorotic acid. The seven biomarkers disclosed by the invention can predict or diagnose gestational diabetes mellitus independently or jointly through plasma detection at the early pregnancy stage, effectively improve the early diagnosis rate of gestational diabetes mellitus and improve disease prognosis, and have clinical use and popularization values.

The invention relates to the technical field of medical detection, in particular to a method for detecting aldosterone, cortisol, deoxycorticosterone and cortisone in plasma through LC-MS / MS. The method comprises the following steps: (a) mixing a plasma sample with an internal standard solution to obtain a mixed solution; (b) adding the mixed solution into the magnetic bead suspension, sequentially carrying out adsorption, weak washing, strong washing and elution, and collecting an eluent; (c) carrying out liquid nitrogen blowing and redissolving on the eluent, and then carrying out LC-MS / MS detection; wherein the surfaces of the magnetic beads are modified with steroid adsorption groups, and the target detection objects are aldosterone, cortisol, deoxycorticosterone and cortisone. The detection method is convenient and simple, the detection efficiency is higher than that of a traditional method, and a feasible solution is provided for promoting popularization and application of the LC-MS / MS technology in clinical practice.

The invention belongs to the technical field of food or medicine, and particularly relates to a preparation method of nattokinase sustained-release microspheres, which comprises the following steps: mixing an emulsifier span-80 with liquid paraffin to obtain an oil phase; adding chitosan into an acetic acid solution to prepare a chitosan water solution as a water phase; dropwise adding the water phase into the oil phase to prepare W / O type emulsion, dropwise adding a cross-linking agent A into the emulsion to obtain chitosan microspheres, and taking a chitosan microsphere nattokinase solution and a cross-linking agent B to obtain nattokinase-chitosan core microspheres; mixing the nattokinase-chitosan core microsphere with a cortisol aptamer solution, and incubating to enable the aptamer to be combined on the surface of the nattokinase-chitosan core microsphere; and adding a casein-PEG copolymer solution and a cross-linking agent C into the microspheres to obtain the cortisol response type intelligent switch microspheres. The nattokinase release amount can be dynamically adjusted day and night, the circadian thrombus risk rhythm is matched, the anti-thrombus effect is remarkably improved, side effects are reduced, and the nattokinase can be widely applied to the fields of functional food and drugs.

Relacorilant is useful in the treatment of hypercortisolism and cancer. Many drugs useful in treating hypercortisolism or cancer are metabolized by CYP2C9 enzymes. The effects of concomitant administration of relacorilant and a CYP2C9 substrate are disclosed herein.Relacorilant potently inhibited CYP2C9 in an in vitro test, indicating that co-administration of relacorilant and a CYP2C9 substrate would be expected to increase the CYP2C9 substrate plasma exposure more than five-fold in vivo. Significant reductions in CYP2C9 substrate doses would be expected to be required when administered with relacorilant.Surprisingly, no such increase in plasma exposure was seen in human studies. Applicant discloses that relacorilant may be safely co-administered with unmodified doses of a CYP2C9 substrate such as, e.g., tolbutamide, glimepiride, and glipizide. Relacorilant and unmodified doses of CYP2C9 substrate such as tolbutamide, glimepiride, and glipizide may be co-administered to treat hypercortisolism, or may be co-administered to a cancer patient.

The application discloses a pyrano pyridine compound, a preparation method, a pharmaceutical composition and application thereof. The application provides a compound as shown in formula (I), a pharmaceutically acceptable salt or a stereoisomer thereof, the compounds have a strong inhibitory effect on aldosterone synthase, have little influence on cortisol synthase, have high selectivity and high safety, and can be used for preventing and / or treating various diseases related to aldosterone.

The present invention belongs to the field of sensor preparation technology, and specifically relates to a cortisol sensor and a preparation method thereof, a wearable device and a preparation method and application thereof. The wearable device provided by the present invention is based on multiple sensors, such as a cortisol sensor and an HRV sensor; the present invention utilizes a self-made cortisol sensor to obtain cortisol data, and at the same time, the present invention combines the analysis of the HRV sensor, which is based on a mature photoplethysmography method, with sensor signal amplification and transmission to achieve another way to obtain heartbeat data. The present invention obtains heartbeat and cortisol concentration data through two pathways, sweat and skin, which can help users more accurately discover and record changes in psychological stress and assess mental stress in a non-invasive manner.

The present invention relates to an oral extract composition comprising Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) extract composition, and its process of preparation. Also relates to the oral extract composition formulation comprising with synergistic combinations of the Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) in suitable doses and at least one nutraceutically and / or pharmaceutically accepted excipient for, improvement, and maintenance of women health more preferably in improving of hot flashes, night sweats, poor sleep, anxiety, sexual dysfunction, mood changes, Genitourinary symptoms in overall improving the quality of life, serum estradiol, progesterone, follicle stimulating hormone (FSH), and cortisol during the period of menopause (perimenopause and menopause). The oral extract composition formulation comprising with synergistic combinations of the Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) in suitable doses and at least one nutraceutically and / or pharmaceutically accepted excipient is used for improving Brain-derived neurotrophic factor (BDNF) levels in women during the period of menopause. The present invention, further related to the extraction process of Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.), also provides the process for the preparation of formulation composition. This invention further relates to the extraction process from Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) either as individual herbs or co-extraction of the two herbs or mixing the extracts at various stages of the individual extracts.

The invention relates to a formula of a tea drink, and relates to the technical field of homology of medicine and food, and the formula is prepared by compounding nine components of double-petal red rose, Hetian jade jujube slices, dried mulberry, longan pulp, Ningxia wolfberry, Xinhui dried orange peel, raspberry, Hunan lotus seed pulp and licorice slices according to a specific proportion. Synergistic interaction is realized through triple action mechanisms, the liver soothing and depression relieving of the double-petal red rose is taken as a regulation and control core, the cortisol level is reduced, and endocrine imbalance is improved; on the basis of benefiting qi and nourishing blood by jujube slices and Chinese wolfberry fruits, dried mulberries and raspberries are enriched with natural folic acid and antioxidant substances, so that the egg cell quality and reproductive microcirculation are improved in a targeted manner; longan-Hunan lotus seed meat is used for warming uterus and tonifying spleen, and dried orange peel-licorice tablets are combined to enhance component absorption and compatibility stability. The preparation method comprises the steps of gradient drying, precise mixing and cobalt-60 irradiation sterilization. According to the scheme, the absorption bottleneck of traditional substitutional tea is broken through, the bioavailability is improved, and the compound sub-health problems of female endocrine disorder, follicular development disorder and cold uterus and weak body are quantitatively solved.

The invention discloses application of a circium japonicum extract. The circium japonicum extract is applied to preparation of products for preventing skin problems caused by cortisol unbalance. The invention also relates to application in preparation of products for relieving skin problems caused by cortisol imbalance. The circium japonicum extract can improve skin problems caused by excessive transformation of cortisol and increase the expression quantity of dermal collagen, skin barrier related protein and skin basement membrane related protein, so that the problems of skin barrier damage, skin sensitivity, wrinkle generation, skin elasticity reduction and the like caused by emotional pressure are effectively relieved.

The present disclosure relates to the use of cortisol, cortisone, cortisone acetate, hydrocortisone, fludrocortisone, prednisolone, prednisone, methylprednisolone, Dehydroepiandrosterone (DHEA), dexamethasone, betamethasone, adrenocorticotropic hormone (ACTH), ganaxolone, tetracosactide or combinations thereof for preventing and / or reducing the risk of Sudden Unexpected Death in Epilepsy (SUDEP). The disclosure further provides pharmaceutical compositions and methods for preventing and / or reducing the risk of SUDEP, particularly in Dravet Syndrome patients.

The invention discloses a wearable molecular imprinting sensor and application thereof in cortisol detection, and belongs to the technical field of electrochemical analysis and detection. The molecular imprinting sensor sequentially comprises a sweat absorbing pad, a three-electrode system, an annular polydimethylsiloxane mold and a medical bonding patch from the skin contact surface to the outside, the front surface of the sweat-absorbent pad is in contact with the skin, and KCl and K3 [Fe (CN) 6] are adsorbed on the back surface of the sweat-absorbent pad; the three-electrode system takes an MIP / AuNWs / PET sensor as a working electrode, a platinum wire as a counter electrode and a silver / silver chloride electrode as a reference electrode; the annular polydimethylsiloxane mold and the medical adhesive patch form a sweat collection chamber. The molecular imprinting sensor based on in-situ growth of gold nanowires (AuNWs) on the porous PET film is used for rapidly detecting the cortisol level in human sweat in real time, natural receptor ligand interaction is simulated through the molecular imprinting technology, and high-specificity recognition of cortisol is achieved.

This invention relates to the field of in vitro diagnostic technology, and more particularly to a method for determining cortisol levels and its application. This invention provides a pretreatment reagent combination for liquid chromatography, a method for pretreating urine samples in liquid chromatography, and a method for detecting cortisol levels in urine. The method for detecting cortisol levels in urine samples provided by this invention offers convenient and rapid pretreatment, and through method validation, demonstrates excellent performance indicators.

The present application relates to a kind of pituitary adenoma simulation teaching method, one aspect, in the case of unified time axis, with the volume of pituitary adenoma as the basis, the hormone level change of corresponding hormone species and visual change condition are associated, can clearly know the curve and numerical value of GH, PRL, ACTH, cortisol and other key hormones, the condition of real-time change with tumor state change, and the real-time synchronization of visual field change and the increase and decrease of tumor size, strengthen the understanding of "space-occupied effect-dysfunction" correlation of the trainee, and the degree of oppression of pituitary adenoma to surrounding tissue is quantified by pressure sensor, the compression phenomenon of pituitary adenoma volume change is directly reflected;Another aspect, for a large number of hormone species data, through the preset data ROI search algorithm, the position of data weak fluctuation can be accurately found, and the corresponding volume change moment of pituitary adenoma helps trainee to locate quickly key time point, improves learning efficiency.

The invention provides a compound feed additive for relieving stress response of marine fishes, which is prepared by the following steps: mixing raw materials such as hermetia illucens protein and the like to prepare a premix, and embedding the premix by using a lac-glycyrrhizic acid system to prepare lac microcapsules; then modifying the fucoidin by adopting glycyrrhizin and gamma-linolenic acid, embedding rhodosporidium palustris and bacillus pumilus by using the fucoidin to prepare compound bacterium nano-particles, and finally mixing the lac microcapsules and the compound bacterium nano-particles in proportion. According to the invention, excessive secretion of cortisol of marine fish can be effectively inhibited, hermetia illucens protein provides rich nutrition and reduces muscle decomposition, and compound microorganisms are beneficial to maintaining intestinal microecological balance and improving the utilization rate of nutrition. The prepared compound feed additive can be directly splashed into a culture or transportation water body or added into a feed, so that the stress reaction of the marine fish is effectively relieved, the survival rate of the marine fish is increased, and powerful support is provided for culture of the marine fish.

The application relates to the technical field of electrochemical detection, in particular to a micro-needle electrochemical biosensor based on an aptamer and a preparation method thereof, which is a three-electrode system, the three-electrode system comprises a working electrode, a reference electrode and a counter electrode, and the base materials of the working electrode, the reference electrode and the counter electrode are all polylactic acid micro-needles with a sputtered gold layer; the modification material of the working electrode comprises 6-mercapto-1-hexanol, 3-aminopropyltriethoxysilane, glutaraldehyde, an amino-modified cortisol aptamer and bovine serum albumin. The micro-needle electrochemical biosensor based on the aptamer and the preparation method thereof are adopted to construct a three-electrode micro-needle system for detecting cortisol in tissue fluid, continuous and painless detection can be realized, the compliance of patients is improved, the detection limit is low, the detection range is wide, the sensitivity is high, and the needs of human body for daily detection of cortisol can be met.

The present application relates to a kind of anti-aging composition for promoting intercellular communication of skin cell and its application, the composition includes the following components: camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside, the weight ratio of the camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside is (1-10):(1-8):(1-6):(0.1-1).The anti-aging composition of the present application can promote the activity of skin communication cell, strengthen intercellular communication and interaction, fully exert the function of cell biology, achieve the effect of anti-aging;And inhibit the damage of cortisol to skin barrier, achieve the effect of repair and emotional stress relief, synergistic effect between components, can achieve the best wrinkle, firming, smoothing and repair effect.

A method for measuring glucose and cortisol levels in earwax, wherein the measured cortisol and glucose levels are interpreted as average cortisol and glucose levels, and a medical device that provides effective, reliable, safe 5 and hygienic self-extraction of earwax.

The invention provides a polianthes tuberosa corm extract and a preparation method and application thereof.The preparation method includes the steps that fresh polianthes tuberosa corm and water are mixed and then smashed, then stirring extraction is conducted at the temperature of 50-70 DEG C, supernate is collected through centrifugation and filtered, and polyhydric alcohol with the volume concentration of 30-50% is added; and the polyol is 1, 3-butanediol or glycerol. According to the tuberose corm extract disclosed by the invention, by inhibiting TNF-alpha induced cortisol excessive secretion and promoting beta-ennorphin release, the dual effects of inhibiting stress and promoting soothing are realized, and the problems of skin inflammation, sensitivity, barrier damage and the like caused by psychological emotion stress are solved.

The invention discloses a composite electrode for rapidly detecting cortisol and application of the composite electrode in rapid detection of cortisol. The composite electrode comprises a laser-induced graphene substrate, a gold nano material layer modified on the laser-induced graphene substrate and a perfluorosulfonic acid polymer modified on the gold nano material layer. The composite electrode does not need an incubation binding step, is high in timeliness, improves the detection rate, can realize real-time analysis, and is stable in film-forming interface; the method is not influenced by the dispersity of a catalytic material, so that the difficulty of the product performance consistency requirement in a quality control link in industrial production is greatly reduced; the electrocatalytic analysis of cortisol can be realized in the PBS buffer solution, and the application range is wider.

The invention discloses a method for detecting cortisol, testosterone and dehydroepiandrosterone in saliva, and belongs to the field of biological medicine. The technical problem to be solved is to provide a method for accurately detecting cortisol, testosterone and dehydroepiandrosterone in saliva. According to the technical scheme, the method for detecting cortisol, testosterone and dehydroepiandrosterone in saliva is characterized in that a magnetic bead method is adopted for sample pretreatment, a to-be-detected sample is obtained through magnetic bead activation, balancing, sample loading, leaching, elution and nitrogen blowing redissolution, and the to-be-detected sample is detected through a liquid chromatography-tandem mass spectrometry method. The contents of cortisol, testosterone and dehydroepiandrosterone in the sample to be detected are obtained. The detection method is accurate and reliable in detection result, simple, convenient and efficient to operate, and meets industrial practical requirements.

The application provides a wearable sweat micro-fluidic analysis chip and a preparation method and application thereof. The wearable sweat micro-fluidic analysis chip is sequentially provided with a sweat inlet layer 1, a branch channel layer 3 and a cover layer 5 from top to bottom. The sweat inlet layer is provided with a sweat inlet 6. The branch channel layer 3 is provided with a detection chamber 10, a channel 17 and a valve chamber 9. The sweat inlet 6, the valve chamber 9, the channel 17 and the detection chamber 10 are sequentially communicated. The cover layer 5 is provided with a sweat outlet 14. A water droplet type polymer valve is placed in the valve chamber 9. The opening time of the water droplet type polymer valve can be flexibly controlled by simply adjusting the polymer concentration. The sweat sample can be sequentially collected, and the contact time of the sweat sample and the detection reagent can be accurately controlled. A reliable premise is provided for subsequent accurate analysis. The application can be applied to the detection of cortisol and the detection of uric acid.

A Lacticaseibacillus rhamnosus MBP01 and a combination thereof for preventing and treating irritable bowel syndrome are provided, which relates to the technical field of microorganism. A deposit number of the Lacticaseibacillus rhamnosus MBP01 is GDMCC NO: 64725. The MBP01 can improve a weight loss trend of IBS model mice, increase food intake and feces water content, alleviate colon tissue damage, downregulate expression levels of IL-1β, TNF-α, PAR-2, and serum CORT and increase an expression level of IL-10. MBP01 with a high concentration can improve an expression level of OCC. Therefore, MBP01 has a certain positive regulatory effect on the intestinal function of IBS model mice and can effectively prevent and treat irritable bowel syndrome.

Carotenoid compositions are described for management, treatment, and / or prevention of stress by reducing cortisol levels and / or improving distribution and / or density of macular pigment and thus improving overall health status. More particularly, a subject in need thereof for the method has disturbed macular pigment optical density or increased levels of cortisol as markers of psychological and physiological stress. Methods are also described of administering macular carotenoid compositions in daily doses of at least or about 0.005 mg / kg body weight of lutein, and at least or about 0.001 mg / kg body weight of meso-zeaxanthin and zeaxanthin isomer along with at least one food grade excipient and evaluating overall health status. The composition may be derived from plant extract containing xanthophylls and / or xanthophylls esters. The composition also reduces cortisol levels in the blood and relieves physiological stress, thus improving overall health status of a subject in need thereof.

The invention relates to the technical field of computers, and discloses an endocrine metabolism index real-time processing method based on edge calculation. The method comprises the following steps: acquiring time sequence data of blood glucose, insulin, cortisol and thyroid stimulating hormone collected by wearable equipment; constructing a lightweight linear state space model of the diagonal dominant sparse state transition matrix; initializing a noise covariance matrix; iterative Kalman filtering based on a sparse matrix inversion algorithm is executed; the observation noise covariance is dynamically adjusted according to the residual error; and when the filtering result exceeds a clinical critical value, triggering local early warning and uploading to the cloud. According to the scheme, the single filtering delay is controlled within 80 milliseconds while the numerical stability is guaranteed, and the early warning timeliness and the system energy efficiency are improved.

This invention discloses a naturally formed nutritional licking brick specifically for musk deer and its preparation method. Using calcium silicate as the skeleton material, it is compounded with yeast culture powder, bentonite, compound probiotics, sweeteners, and flavoring agents. The resulting licking brick continuously releases soluble nutrients and active bacteria during the musk deer's licking process. The specific combination of probiotics and yeast culture powder components effectively improves the intestinal immunity of captive musk deer, significantly reduces intestinal inflammation, and simultaneously lowers cortisol levels in feces, alleviating stress responses. Using yeast culture powder, calcium silicate, and bentonite as a composite coagulant, the licking brick prepared by mold casting exhibits optimal moisture resistance during storage. Furthermore, the licking brick has a smooth appearance, a compact and sturdy structure, excellent molding performance, and retains its structural integrity even after immersion in water, making it suitable for preparing products related to improving the intestinal health and immunity of musk deer.

The invention is suitable for the technical field of biological medicine, and provides an active composition for relieving fatigue and application of the active composition. The composition is prepared from a silkworm chrysalis-derived mitochondrial repair peptide, a chickpea-derived neuromodulation peptide, a haematococcus pluvialis-derived phagocytosis peptide, a phosphatidylcholine-lipoic acid conjugate, a whey protein-chitosan copolymer and an ergothioneine-selenium compound in a synergistic manner. Through the synergistic effect of four mechanisms of energy metabolism activation, endocrine balance, mitochondrial autophagy repair and oxidative stress removal, physiological fatigue and stress fatigue are effectively and synchronously relieved. After targeted modification, the utilization rate of macamides is remarkably increased, and the composition can remarkably prolong exercise tolerance, reduce cortisol level, promote GABA synthesis, recover mitochondrial function and greatly shorten fatigue recovery time.

The invention provides a composition for relieving beef cattle transportation stress syndrome and application thereof, the composition is prepared by premixing dexmedetomidine hydrochloride, gastrodin and normal saline for injection according to the mass concentration ratio, the concentration of dexmedetomidine hydrochloride is 60-65 g / mL, and the concentration of gastrodin is 20-30 mg / mL. The composition is applied to beef cattle before transportation, the composition is intramuscularly injected, and the total injection volume is 10-20 mL. After the composition is injected before beef cattle transportation, immune dysfunction can be effectively improved, excessive HPA axis activation is inhibited, cortisol secretion is reduced, oxidative damage is relieved, and the stability of intestinal flora is maintained, so that inflammation is relieved, nervous excitability is reduced, calming and anxiety resisting effects are achieved, beef cattle transportation stress response is reduced, and the beef cattle transportation effect is improved. And the method is simple to operate and low in cost, and provides theoretical and practical support for preventing the beef cattle transportation stress syndrome.

The invention provides a cortisol compound antibody. Amino acid sequences of a heavy chain CDR1 sequence, a heavy chain CDR2 sequence and a heavy chain CDR3 sequence of the cortisol compound antibody are shown as SEQ ID NO: 1-3; amino acid sequences of a light chain CDR1 sequence, a light chain CDR2 sequence and a light chain CDR3 sequence are shown as SEQ ID NO: 4-6. The antibody can be used for preparing a cortisol sandwich method detection reagent and detecting a clinical sample, the sensitivity of the detected sample is high, and the detection linear range is wide.

The invention discloses prevention and treatment of diseases by stimulating TRPV1 + sensory nerves. Specifically, the invention discloses application of a substance for stimulating TRPV1 + sensory nerves in preparation of products for prevention, treatment or adjuvant treatment of inflammatory diseases, autonomic nerve dysfunction diseases or adrenal insufficiency diseases, and application of the substance for stimulating TRPV1 + sensory nerves in preparation of products for inhibiting proinflammatory cytokines and products for promoting anti-inflammatory cytokines. The TRPV1 ion channel agonist disclosed by the invention can obviously and effectively inhibit the expression of numerous proinflammatory cytokines and obviously improve the expression of anti-inflammatory cytokines IL10, so that not only is the physiological status of an inflammatory mouse obviously improved, but also the survival rate of the inflammatory mouse is obviously improved; meanwhile, the spleen function can be adjusted, expression of spleen pro-inflammatory genes is remarkably inhibited, generation of catecholamine is increased, and secretion of cortisol hormones is promoted. The invention has wide clinical application value in prevention and treatment of related diseases.

The invention discloses application of a mulberry root bark extract, and particularly relates to application of the mulberry root bark extract in preparation of a product for improving skin cortisol unbalance caused by pressure stress. The mulberry root bark extract provided by the invention can improve skin problems caused by excessive transformation of cortisol due to pressure stress, and improve the expression quantity of skin collagen, skin barrier related protein and skin basement membrane related protein, so that skin barrier damage, skin sensitivity, skin aging, skin aging and the like caused by emotional pressure are effectively relieved. Wrinkles are generated; the skin elasticity is reduced and the like.