170 results about "Cortisol synthesis" patented technology

The invention discloses a cortisol and sodium ion difunctional detection sensor and a preparation method thereof, and belongs to the technical field of sensing detection, the cortisol and sodium ion difunctional detection sensor comprises a substrate layer, a sodium ion detection module and a cortisol detection module, the bionic micro-fluidic unit is provided with a plurality of runner holes, and an embedded capillary pump structure is arranged in each runner hole so as to guide liquid to be detected to the sodium ion detection module and the cortisol detection module; and the shielding layer covers the surface of the bionic micro-fluidic unit. The sodium ion detection module and the cortisol detection module are specifically designed, so that wide-range detection of sodium ion concentration and low-concentration detection of cortisol are realized, a high-altitude low-pressure environment of a pilot can be matched, the sodium ion concentration and the cortisol concentration of the pilot can be accurately detected, and the detection accuracy is improved. Accurate detection of the signs of the pilot in the high-altitude flight process is achieved.

The invention discloses a pyranopyridine compound as well as a preparation method, a pharmaceutical composition and application thereof. The invention provides a compound as shown in a formula (I), a pharmaceutically acceptable salt or a stereoisomer thereof, and the compound has a relatively strong inhibition effect on aldosterone synthetase, almost has no influence on cortisol synthetase, has high selectivity and relatively high safety, and can be used for preventing and / or treating various diseases related to aldosterone. # imgabs0 #

The invention belongs to the technical field of gestational diabetes diagnosis marker screening, and particularly relates to a biomarker for predicting or diagnosing gestational diabetes and application of the biomarker. The invention discloses a biomarker for predicting or diagnosing gestational diabetes mellitus, the biomarker is selected from one or more of hypoxanthine, cortisol, dihydrotachysterol and cycloleucine, and more preferably, the biomarker comprises hypoxanthine, cortisol, dihydrotachysterol, (3R, 4R)-D-ketolactone, creatine, cycloleucine and L-hydroorotic acid. The seven biomarkers disclosed by the invention can predict or diagnose gestational diabetes mellitus independently or jointly through plasma detection at the early pregnancy stage, effectively improve the early diagnosis rate of gestational diabetes mellitus and improve disease prognosis, and have clinical use and popularization values.

The invention relates to the technical field of medical detection, in particular to a method for detecting aldosterone, cortisol, deoxycorticosterone and cortisone in plasma through LC-MS / MS. The method comprises the following steps: (a) mixing a plasma sample with an internal standard solution to obtain a mixed solution; (b) adding the mixed solution into the magnetic bead suspension, sequentially carrying out adsorption, weak washing, strong washing and elution, and collecting an eluent; (c) carrying out liquid nitrogen blowing and redissolving on the eluent, and then carrying out LC-MS / MS detection; wherein the surfaces of the magnetic beads are modified with steroid adsorption groups, and the target detection objects are aldosterone, cortisol, deoxycorticosterone and cortisone. The detection method is convenient and simple, the detection efficiency is higher than that of a traditional method, and a feasible solution is provided for promoting popularization and application of the LC-MS / MS technology in clinical practice.

The invention relates to an anti-aging composition for promoting skin intercellular communication and application thereof, and the composition comprises the following components: a camellia nitidissima flower extract, a bacillus fermentation product, an European basswood extract and safflower glucoside, the weight ratio of the golden camellia flower extract to the bacillus fermentation product to the European basswood extract to the safflower glucoside is (1-10): (1-8): (1-6): (0.1-1). The anti-aging composition provided by the invention can promote the activity of skin communication cells, enhance intercellular communication and interaction, give full play to the biological function of cells, and achieve the anti-aging effect; the composition has the effects of removing wrinkles, tightening, smoothing and repairing, can inhibit damage of cortisol to skin barriers, achieves the effects of repairing and relieving mood pressure, and can achieve the optimal effects of removing wrinkles, tightening, smoothing and repairing due to mutual synergism of the components.

The invention belongs to the technical field of food or medicine, and particularly relates to a preparation method of nattokinase sustained-release microspheres, which comprises the following steps: mixing an emulsifier span-80 with liquid paraffin to obtain an oil phase; adding chitosan into an acetic acid solution to prepare a chitosan water solution as a water phase; dropwise adding the water phase into the oil phase to prepare W / O type emulsion, dropwise adding a cross-linking agent A into the emulsion to obtain chitosan microspheres, and taking a chitosan microsphere nattokinase solution and a cross-linking agent B to obtain nattokinase-chitosan core microspheres; mixing the nattokinase-chitosan core microsphere with a cortisol aptamer solution, and incubating to enable the aptamer to be combined on the surface of the nattokinase-chitosan core microsphere; and adding a casein-PEG copolymer solution and a cross-linking agent C into the microspheres to obtain the cortisol response type intelligent switch microspheres. The nattokinase release amount can be dynamically adjusted day and night, the circadian thrombus risk rhythm is matched, the anti-thrombus effect is remarkably improved, side effects are reduced, and the nattokinase can be widely applied to the fields of functional food and drugs.

The invention relates to the technical field of medical detection, in particular to an endocrine hypertension screening method based on immunological analysis, which comprises the following steps: collecting a random urine sample, removing interferents by using a composite nano adsorbent, and synchronously detecting hormones such as aldosterone, methoxyadrenaline and cortisol and metabolites thereof by using a high-throughput immunity analyzer; integrating detection data based on a dynamic threshold algorithm to generate a screening report, carrying out weighted scoring on positive samples in combination with clinical features, screening high-risk candidate samples, carrying out 24-hour urine retest on the high-risk samples, and if a retest result is still positive, carrying out referral treatment to a specialized department for disease cause definite diagnosis; according to the method, the technical problems of complex sample treatment, low multi-step efficiency and high false positive rate in the prior art are solved, efficient and accurate screening of endocrine hypertension is realized, and reliable support is provided for early diagnosis and targeted treatment.

Relacorilant is useful in the treatment of hypercortisolism and cancer. Many drugs useful in treating hypercortisolism or cancer are metabolized by CYP2C9 enzymes. The effects of concomitant administration of relacorilant and a CYP2C9 substrate are disclosed herein.Relacorilant potently inhibited CYP2C9 in an in vitro test, indicating that co-administration of relacorilant and a CYP2C9 substrate would be expected to increase the CYP2C9 substrate plasma exposure more than five-fold in vivo. Significant reductions in CYP2C9 substrate doses would be expected to be required when administered with relacorilant.Surprisingly, no such increase in plasma exposure was seen in human studies. Applicant discloses that relacorilant may be safely co-administered with unmodified doses of a CYP2C9 substrate such as, e.g., tolbutamide, glimepiride, and glipizide. Relacorilant and unmodified doses of CYP2C9 substrate such as tolbutamide, glimepiride, and glipizide may be co-administered to treat hypercortisolism, or may be co-administered to a cancer patient.

The application discloses a pyrano pyridine compound, a preparation method, a pharmaceutical composition and application thereof. The application provides a compound as shown in formula (I), a pharmaceutically acceptable salt or a stereoisomer thereof, the compounds have a strong inhibitory effect on aldosterone synthase, have little influence on cortisol synthase, have high selectivity and high safety, and can be used for preventing and / or treating various diseases related to aldosterone.

The invention discloses an emotion beautifying composition containing a camellia nitidissima flower extract and application of the emotion beautifying composition, and relates to the technical field of cosmetics. The invention provides an emotional beautifying composition. The emotional beautifying composition is prepared from the following components in parts by weight: 0.1 to 5 parts of purple coneflower extract, 0.01 to 2 parts of tephros glauca seed extract, 0.01 to 3 parts of golden camellia flower extract and 0.1 to 3 parts of lactobacillus fermentation lysate. Aiming at the core problem of skin barrier gene (FLG / LOR) expression inhibition caused by cortisol rhythm disorder caused by emotional pressure, the invention provides a solution scheme of three-stage linkage of cortisol rhythm synchronization, barrier gene repair and nerve sensitivity regulation.

The invention discloses a stem cell composition and application thereof in regulating sub-health. The stem cell composition comprises mesenchymal stem cells (MSCs) modified by fusion polypeptide and an anti-IL-6R single domain antibody. The amino acid sequence of the fusion polypeptide is as shown in SEQ ID NO.1, and the fusion polypeptide is modified on the surface of stem cells through click chemistry, so that the chemotactic migration ability, collagen matrix affinity and inflammation tolerance of the stem cells are remarkably improved. The anti-IL-6R single-domain antibody is obtained through phage display screening, the VHH gene of the anti-IL-6R single-domain antibody is shown as SEQ ID NO.2, and the anti-IL-6R single-domain antibody has high affinity and activity of inhibiting an IL-6 / STAT3 signal channel. Animal experiments show that the composition can significantly reduce inflammatory factor (IL-6) and cortisol levels of chronic stress model mice, improve oxidation resistance (SOD) and recover immune homeostasis, and provides an innovative treatment strategy for adjustment of sub-health status.

The present Invention relates to a newly designed product, i.e. substance (B+C) that combines two different mechanisms of action that allow the control of aldosterone synthesis and secretion without compromising the metabolism of cortisol (corticosterone In rats) in the.tone glomerulosa of the adrenal gland. The innovative design of the described molecule enables ths individual use of substance (B+C) in the treatment of diseases such as hyper and hypo aldosteronism, Conn's syndrome and cytokine storm caused by Covid infection or as co-therapy with corticosteroids in the treatment of a whole range of immunological diseases. The innovative design of Substance (8+C) has an additional practical and economic advantage because the patient receives both components of substance (B+C) simultaneously with just one application, regardless of the medicine formulation. This not only simplifies the medicine synthesis, dosing and clinical research, but also the application of the medicine in clinical practice. For successful and selective control of aldosterone synthesis, which in nature also means selective control of blood pressure, it was necessary to combine the two substances described in order to ensure action in patients with elevated and / or towered blood pressure with one application of Substance (B+C). This feature of Substance (B+C) is important, especially in the treatment of patients with permanently impaired aldosterone synthesis, such as Conn's disease and similar autoimmune diseases, or in combmatton with corticosteroids where prolonged or repeated corticosteroid therapy is indicated. Finally, this invention is extremely important because It enables the safe treatment of a large group of patients with hypertension who also have secondary diseases of the immune system, various inflammations or viral Infections such as Covid where corticosteroid therapy is indicated, but difficult to implement in practice due to the negative effect of corticosteroids on blood pressure.

The present invention belongs to the field of sensor preparation technology, and specifically relates to a cortisol sensor and a preparation method thereof, a wearable device and a preparation method and application thereof. The wearable device provided by the present invention is based on multiple sensors, such as a cortisol sensor and an HRV sensor; the present invention utilizes a self-made cortisol sensor to obtain cortisol data, and at the same time, the present invention combines the analysis of the HRV sensor, which is based on a mature photoplethysmography method, with sensor signal amplification and transmission to achieve another way to obtain heartbeat data. The present invention obtains heartbeat and cortisol concentration data through two pathways, sweat and skin, which can help users more accurately discover and record changes in psychological stress and assess mental stress in a non-invasive manner.

The present invention relates to an oral extract composition comprising Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) extract composition, and its process of preparation. Also relates to the oral extract composition formulation comprising with synergistic combinations of the Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) in suitable doses and at least one nutraceutically and / or pharmaceutically accepted excipient for, improvement, and maintenance of women health more preferably in improving of hot flashes, night sweats, poor sleep, anxiety, sexual dysfunction, mood changes, Genitourinary symptoms in overall improving the quality of life, serum estradiol, progesterone, follicle stimulating hormone (FSH), and cortisol during the period of menopause (perimenopause and menopause). The oral extract composition formulation comprising with synergistic combinations of the Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) in suitable doses and at least one nutraceutically and / or pharmaceutically accepted excipient is used for improving Brain-derived neurotrophic factor (BDNF) levels in women during the period of menopause. The present invention, further related to the extraction process of Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.), also provides the process for the preparation of formulation composition. This invention further relates to the extraction process from Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) either as individual herbs or co-extraction of the two herbs or mixing the extracts at various stages of the individual extracts.

The invention relates to a formula of a tea drink, and relates to the technical field of homology of medicine and food, and the formula is prepared by compounding nine components of double-petal red rose, Hetian jade jujube slices, dried mulberry, longan pulp, Ningxia wolfberry, Xinhui dried orange peel, raspberry, Hunan lotus seed pulp and licorice slices according to a specific proportion. Synergistic interaction is realized through triple action mechanisms, the liver soothing and depression relieving of the double-petal red rose is taken as a regulation and control core, the cortisol level is reduced, and endocrine imbalance is improved; on the basis of benefiting qi and nourishing blood by jujube slices and Chinese wolfberry fruits, dried mulberries and raspberries are enriched with natural folic acid and antioxidant substances, so that the egg cell quality and reproductive microcirculation are improved in a targeted manner; longan-Hunan lotus seed meat is used for warming uterus and tonifying spleen, and dried orange peel-licorice tablets are combined to enhance component absorption and compatibility stability. The preparation method comprises the steps of gradient drying, precise mixing and cobalt-60 irradiation sterilization. According to the scheme, the absorption bottleneck of traditional substitutional tea is broken through, the bioavailability is improved, and the compound sub-health problems of female endocrine disorder, follicular development disorder and cold uterus and weak body are quantitatively solved.

The present invention belongs to the technical field of medicine and food homologous compositions, and discloses a composition for enhancing exercise endurance and its applications. It is found that the composition has the following effects: (1) After 30 minutes of intervention with a high dose of the composition, exercise endurance can be significantly enhanced, and it can affect exercise endurance by regulating the levels of hormones such as testosterone and cortisol. (2) Intervention with the composition for 14 days can significantly prolong the swimming time to exhaustion under negative gravity in mice, reduce the accumulation of harmful metabolites, thereby increasing the body's energy metabolism and reducing the degree of fatigue of the body. (3) Intervention with a high dose of the composition for 28 days can significantly increase exercise endurance, and by increasing the muscle glycogen reserve and reducing the accumulation of harmful metabolites such as urea nitrogen and lactic acid, the effect of improving exercise endurance is achieved.

Compositions and methods for converting at least one polyphenol to protocatechuic acid (PCA) using Bacillus subtilis 1579, or active variants thereof, are provided. Conversion of polyphenols, such as quercetin, to PCA can decrease inflammation, decrease cortisol levels, and increase milk quality and milk production quantity in milk-producing agricultural animals. Accordingly, provided herein are compositions comprising B. subtilis 1579, or an active variant thereof, for administration to humans for decreasing inflammation or for administration to milk-producing agricultural animals for decreasing inflammation, decreasing cortisol levels, and increasing milk quality and milk production.

The invention discloses application of a circium japonicum extract. The circium japonicum extract is applied to preparation of products for preventing skin problems caused by cortisol unbalance. The invention also relates to application in preparation of products for relieving skin problems caused by cortisol imbalance. The circium japonicum extract can improve skin problems caused by excessive transformation of cortisol and increase the expression quantity of dermal collagen, skin barrier related protein and skin basement membrane related protein, so that the problems of skin barrier damage, skin sensitivity, wrinkle generation, skin elasticity reduction and the like caused by emotional pressure are effectively relieved.

The invention discloses an after-sun repair composition for improving emotion, application, cosmetics and a preparation method of the cosmetics. The after-sun repair composition is prepared from alfalfa extract and zanthoxylum armatum fruit extract, the mass ratio of the alfalfa extract to the zanthoxylum armatum fruit extract is (0.1-2%): (0.1-2%). According to the after-sun repair composition for improving the emotion and the application thereof, the cosmetic and the preparation method of the after-sun repair composition, through the synergistic effect of the alfalfa extract and the zanthoxylum armatum fruit extract, the after-sun repair composition can meet the requirements for water replenishing, moisturizing, relieving and anti-inflammation, and meanwhile the effect of improving the emotion is achieved by reducing the cortisol level in skin cells and increasing release of dopamine. The effects of improving the mood and reducing the damage to the skin caused by the negative mood generated by the appearance change of the sun-dried skin are achieved.

The present disclosure relates to the use of cortisol, cortisone, cortisone acetate, hydrocortisone, fludrocortisone, prednisolone, prednisone, methylprednisolone, Dehydroepiandrosterone (DHEA), dexamethasone, betamethasone, adrenocorticotropic hormone (ACTH), ganaxolone, tetracosactide or combinations thereof for preventing and / or reducing the risk of Sudden Unexpected Death in Epilepsy (SUDEP). The disclosure further provides pharmaceutical compositions and methods for preventing and / or reducing the risk of SUDEP, particularly in Dravet Syndrome patients.

The invention discloses a wearable molecular imprinting sensor and application thereof in cortisol detection, and belongs to the technical field of electrochemical analysis and detection. The molecular imprinting sensor sequentially comprises a sweat absorbing pad, a three-electrode system, an annular polydimethylsiloxane mold and a medical bonding patch from the skin contact surface to the outside, the front surface of the sweat-absorbent pad is in contact with the skin, and KCl and K3 [Fe (CN) 6] are adsorbed on the back surface of the sweat-absorbent pad; the three-electrode system takes an MIP / AuNWs / PET sensor as a working electrode, a platinum wire as a counter electrode and a silver / silver chloride electrode as a reference electrode; the annular polydimethylsiloxane mold and the medical adhesive patch form a sweat collection chamber. The molecular imprinting sensor based on in-situ growth of gold nanowires (AuNWs) on the porous PET film is used for rapidly detecting the cortisol level in human sweat in real time, natural receptor ligand interaction is simulated through the molecular imprinting technology, and high-specificity recognition of cortisol is achieved.

This invention relates to the field of in vitro diagnostic technology, and more particularly to a method for determining cortisol levels and its application. This invention provides a pretreatment reagent combination for liquid chromatography, a method for pretreating urine samples in liquid chromatography, and a method for detecting cortisol levels in urine. The method for detecting cortisol levels in urine samples provided by this invention offers convenient and rapid pretreatment, and through method validation, demonstrates excellent performance indicators.

The present application relates to a kind of pituitary adenoma simulation teaching method, one aspect, in the case of unified time axis, with the volume of pituitary adenoma as the basis, the hormone level change of corresponding hormone species and visual change condition are associated, can clearly know the curve and numerical value of GH, PRL, ACTH, cortisol and other key hormones, the condition of real-time change with tumor state change, and the real-time synchronization of visual field change and the increase and decrease of tumor size, strengthen the understanding of "space-occupied effect-dysfunction" correlation of the trainee, and the degree of oppression of pituitary adenoma to surrounding tissue is quantified by pressure sensor, the compression phenomenon of pituitary adenoma volume change is directly reflected;Another aspect, for a large number of hormone species data, through the preset data ROI search algorithm, the position of data weak fluctuation can be accurately found, and the corresponding volume change moment of pituitary adenoma helps trainee to locate quickly key time point, improves learning efficiency.

The invention provides a compound feed additive for relieving stress response of marine fishes, which is prepared by the following steps: mixing raw materials such as hermetia illucens protein and the like to prepare a premix, and embedding the premix by using a lac-glycyrrhizic acid system to prepare lac microcapsules; then modifying the fucoidin by adopting glycyrrhizin and gamma-linolenic acid, embedding rhodosporidium palustris and bacillus pumilus by using the fucoidin to prepare compound bacterium nano-particles, and finally mixing the lac microcapsules and the compound bacterium nano-particles in proportion. According to the invention, excessive secretion of cortisol of marine fish can be effectively inhibited, hermetia illucens protein provides rich nutrition and reduces muscle decomposition, and compound microorganisms are beneficial to maintaining intestinal microecological balance and improving the utilization rate of nutrition. The prepared compound feed additive can be directly splashed into a culture or transportation water body or added into a feed, so that the stress reaction of the marine fish is effectively relieved, the survival rate of the marine fish is increased, and powerful support is provided for culture of the marine fish.

The application relates to the technical field of electrochemical detection, in particular to a micro-needle electrochemical biosensor based on an aptamer and a preparation method thereof, which is a three-electrode system, the three-electrode system comprises a working electrode, a reference electrode and a counter electrode, and the base materials of the working electrode, the reference electrode and the counter electrode are all polylactic acid micro-needles with a sputtered gold layer; the modification material of the working electrode comprises 6-mercapto-1-hexanol, 3-aminopropyltriethoxysilane, glutaraldehyde, an amino-modified cortisol aptamer and bovine serum albumin. The micro-needle electrochemical biosensor based on the aptamer and the preparation method thereof are adopted to construct a three-electrode micro-needle system for detecting cortisol in tissue fluid, continuous and painless detection can be realized, the compliance of patients is improved, the detection limit is low, the detection range is wide, the sensitivity is high, and the needs of human body for daily detection of cortisol can be met.

The present application relates to a kind of anti-aging composition for promoting intercellular communication of skin cell and its application, the composition includes the following components: camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside, the weight ratio of the camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside is (1-10):(1-8):(1-6):(0.1-1).The anti-aging composition of the present application can promote the activity of skin communication cell, strengthen intercellular communication and interaction, fully exert the function of cell biology, achieve the effect of anti-aging;And inhibit the damage of cortisol to skin barrier, achieve the effect of repair and emotional stress relief, synergistic effect between components, can achieve the best wrinkle, firming, smoothing and repair effect.

The invention discloses a system and a method for detecting the level of cortisol in urine and application, and relates to the technical field of detection of the level of cortisol in urine. The detection system comprises a 24h urocortisol related data and urocortisol correlation analysis module. Wherein the 24-hour urocortisol related data and urocortisol correlation analysis module is based on a monitored 24-hour urocortisol level value after a patient takes hydrocortisone acetate, a 24-hour urocortisol related data level value before and 1 hour after the patient takes hydrocortisone acetate at 8 o'clock in the morning, and a 24-hour urocortisol related data level value before and 1 hour after the patient takes hydrocortisone acetate at 4 o'clock in the afternoon; and performing multivariate linear correlation analysis to obtain a linear relation. The urine cortisol level is calculated according to the linear relational expression, the treatment effect of hormone drugs, especially hydrocortisone acetate, on hypopituitary function can be accurately monitored, and the phenomenon that in the prior art, all urine within 24 h is collected and then cortisol detection is conducted, so that the result is not accurate is avoided.

A method for measuring glucose and cortisol levels in earwax, wherein the measured cortisol and glucose levels are interpreted as average cortisol and glucose levels, and a medical device that provides effective, reliable, safe 5 and hygienic self-extraction of earwax.

Disclosed in the present invention are a pyrano-pyridine compound, a preparation method therefor, a pharmaceutical composition thereof and the use thereof. Provided in the present invention are a compound as shown in formula (I), a pharmaceutically acceptable salt thereof or a stereoisomer thereof. These compounds have a stronger inhibitory effect on aldosterone synthetase, but have almost no influence on cortisol synthetase, have high selectivity and higher safety, and can be used for preventing and / or treating various diseases related to aldosterone.

The invention provides a polianthes tuberosa corm extract and a preparation method and application thereof.The preparation method includes the steps that fresh polianthes tuberosa corm and water are mixed and then smashed, then stirring extraction is conducted at the temperature of 50-70 DEG C, supernate is collected through centrifugation and filtered, and polyhydric alcohol with the volume concentration of 30-50% is added; and the polyol is 1, 3-butanediol or glycerol. According to the tuberose corm extract disclosed by the invention, by inhibiting TNF-alpha induced cortisol excessive secretion and promoting beta-ennorphin release, the dual effects of inhibiting stress and promoting soothing are realized, and the problems of skin inflammation, sensitivity, barrier damage and the like caused by psychological emotion stress are solved.