Method for converting rice straw crude fibers into crude fat through grease microbial fermentation

Abstract

The invention discloses a method for converting crude fibers of rice straws into crude fat through grease microbial fermentation. In order to reduce the crude fiber of the rice straw and increase the crude fat content of the rice straw, the feed is prepared by taking the rice straw as a substrate and assisting agricultural by-products such as wheat bran, rapeseed meal and peeled sweet potato powder through grease microorganism semi-solid state fermentation. According to a single-factor test scheme, fermentation microorganisms including fermentative trichosporon CICC1368, mortierella chrysosphaerella As3.3410, saccharomyces cerevisiae YJZP01 and rhodotorula graminis H124 are subjected to single-factor test optimization, a fermentation substrate is rice straw as a main material, wheat bran, rapeseed meal or peeled sweet potatoes as an auxiliary material, and the proportion of the auxiliary material is (0 to 10 percent, 20 percent, 30 percent, 40 percent and 50 percent). The method comprises the following steps: carrying out an orthogonal test on fermentation condition loading capacity (20, 50, 80g / 250mL triangular flask), material-to-water ratio (0.50, 1.00, 1.50), fermentation days (5, 10, 20%), inoculation proportion (5, 10, 20%) and addition of citric acid (0.2, 0.5, 0.8%), KH2PO4 (0.1, 0.2, 0.3%), urea (0.2, 0.5, 0.8%) and calcium chloride (0.01, 0.02, 0.03%) to fermentation substrates, and obtaining the optimal fermentation condition by taking the crude fat increasing range as a target. Under the optimal condition, the crude fat content of the rice straw is increased by 0.603% from 0.111%, and the increase rate reaches 5 times.

Description

technical field [0001] The invention relates to a method for converting rice straw crude fiber into crude fat through oil microbial fermentation, which belongs to the field of microbial fermentation production, in particular to the production of biological feed that improves the nutritional value of rice straw through microbial fermentation, and belongs to the technical field of biological feed. Background technique [0002] With the change of people's dietary structure, the demand for meat, eggs and dairy products has increased sharply, and the breeding industry has developed rapidly. The rapid development of the breeding industry has intensified the demand for energy feed such as corn. The energy feeds used for livestock and poultry production, such as corn, soybean oil, and rapeseed oil, are also food for humans. In order to avoid conflicts between humans and livestock for food, it is necessary to vigorously develop unconventional feeds for livestock production. In 2018,...

AI Technical Summary

Problems solved by technology

Straw returning to the field is likely to produce harmful substances that poison the rice root system and cause agricultural losses; incineration and random discarding cause environmental pollution

Rice straw Crude fiber content is as high as 60% or more, crude protein only accounts for 3-6%, poor palatability, low animal digestibility, and low overall nutritional value, which limits the way of feed

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