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Complex microbial inoculant suitable for silage corn in Ningxia areas as well as preparation method and application

A technology of compound bacterial agent and silage corn, applied in the field of animal husbandry, can solve the problems of not being able to adapt to the climate environment, high cost of adding bacterial agent, low activity of lactic acid bacteria, etc., achieve high fermentation quality and nutritional value, and improve total energy. , the effect of increasing crude fat content

Inactive Publication Date: 2020-09-18
NINGXIA UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many commercial silage microbial inoculants in the market, but these microbial inoculants are mainly single strains of lactic acid bacteria, and there are few strains for the climate and environment in Ningxia, which cannot be well adapted to Ningxia's winter drought and cold during the silage process. In the climate environment, the activity of lactic acid bacteria is low after adding, and the silage effect is not good; at the same time, there is the problem of high cost of adding bacteria

Method used

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  • Complex microbial inoculant suitable for silage corn in Ningxia areas as well as preparation method and application
  • Complex microbial inoculant suitable for silage corn in Ningxia areas as well as preparation method and application
  • Complex microbial inoculant suitable for silage corn in Ningxia areas as well as preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The isolation and identification of embodiment 1 lactic acid bacteria and yeast

[0027] Take 50 g of silage corn samples in the ultra-clean workbench, put them into a sterilized beaker, add 200 mL of sterile saline, seal it, place it on a shaker, and shake it slowly at 120 r / min for 2 hours. Filter the silage residue with a layer of sterile gauze, centrifuge the filtrate at 12000r / min for 30min, resuspend the sediment with a little sterile saline, select 10 -7 、10 -8 、10 -9 For three dilution gradients, 0.1 mL of liquid was drawn for each dilution, spread on MRS solid medium and YPD medium respectively, and cultured at 37°C for 24-72 hours.

[0028] After colonies are formed on MRS solid medium and YPD medium, observe their morphology in the ultra-clean workbench, colonies in MRS medium, and isolate lactic acid bacteria according to the method introduced in the literature "Lactic Acid Bacteria Classification and Identification and Experimental Methods" Identificatio...

Embodiment 2

[0029] The mensuration of embodiment 2 strain properties

[0030] The lactic acid bacteria and saccharomycetes isolated in Example 1 are subjected to performance tests to finally determine the lactic acid bacteria and saccharomyces used in the present invention.

[0031] The performance test method is:

[0032] 1. Determination of acid production rate of lactic acid bacteria

[0033] The 24h culture solution of lactic acid bacteria was used to inoculate the MRS liquid medium with a 3% inoculation amount, and cultivated in a 37°C incubator. From inoculation 0h, the pH value of the lactic acid bacteria fermentation broth was measured every 2h, with different fermentation times (h) as the abscissa, and the pH value of the fermentation broth as the ordinate, the acid production rate curve was drawn. Determination time 30h. For test results see figure 1 .

[0034] 2. Determination of growth performance of lactic acid bacteria

[0035] Use the culture solution of lactic acid b...

Embodiment 3

[0039] The preparation of embodiment 3 composite bacterial agents

[0040] The lactic acid bacteria and yeast strains screened in Example 2 were fermented in a microbial fermenter to make freeze-dried powder, and the freeze-dried powder was compounded into a composite bacterial agent. The ratio of live bacteria of lactic acid bacteria to yeast was 1:1 , the live bacteria content of the compound bacterial agent is about 10 9 CFU / g.

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Abstract

The invention relates to the technical field of animal husbandry, and particularly discloses a complex microbial inoculant suitable for silage corn in Ningxia areas as well as a preparation method andan application. The complex microbial inoculant suitable for silage corn in the Ningxia areas comprises lactic acid bacteria and saccharomycetes or fermentation products of the lactic acid bacteria and the saccharomycetes. The complex microbial inoculant can significantly reduce the content of neutral detergent fibers and acidic detergent fibers and the content of coarse ash in a silage corn feed, increase the content of coarse fat in the silage and improve the total energy of the silage. Meanwhile, the palatability of the silage corn feed is improved, and it is guaranteed that the silage corn feed has relatively high fermentation quality and nutritional value.

Description

technical field [0001] The invention relates to the technical field of animal husbandry, in particular to a compound bacterial agent suitable for silage corn in Ningxia area, a preparation method and application. Background technique [0002] Corn is one of the three major food crops in Ningxia, and it is also the main feed crop in Ningxia. As a high-quality feed source for ruminants, corn has the advantages of high nutritional value, good palatability, high digestibility, and long storage period after being made into silage. It has become the main feed source for ruminants in Ningxia in winter and the demand is increasing. However, in the production process of corn silage, there are problems to be solved urgently in the production such as nutrient loss and aerobic corruption. [0003] The use of silage additives is one of the important measures to improve the quality of silage. Microbial inoculant is a new type of silage additive that is widely used. Its main function is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K30/18C12N1/20C12N1/16
CPCA23K10/12A23K30/18C12N1/20C12N1/16
Inventor 段子渊王小平王小琪张志超杨果张瑞莹
Owner NINGXIA UNIVERSITY
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